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Culture of Spirulina Platensis in Various Concentrations of Papaya Skin Powder Medium

M.G.U. Sharker
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M.I. Miah
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M.M. Rahman
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M.A.B. Habib
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The growth performance of Spirulina platensis was studied in various concentrations viz., 0.3, 0.4 and 0.5 g/L of papaya skin powder medium (PSPM) and Kosaric Medium (KM) in the laboratory for three months. Each trial was carried out for a period of 12 days with three replications. The growth rate of S. platensis was found to vary in different media. The initial cell weight of S. platensis was 4.0 mg/L which attained a maximum weight of 0.720 g/L in KM followed by 0.693, 0.578 and 0.430 g/L in 0.3, 0.4 and 0.5 g/L of PSPM, respectively on 8th day of culture. Similar trend was observed in the case of chlorophyll a content of S. platensis. The proximate composition of papaya skin powder was analyzed and found that the amount of protein was 20.25%, lipid 3.85%, ash 9.31%, NFE 50.49% and moisture 16.10%. The result indicated that the growth rate of S. platensis was significantly (p<0.01) higher in 0.3 g/L concentration of PSPM than other concentrations of PSPM. The physico-chemical parameters viz., temperature (30.06 °C), light intensity (2110 lux/m2/s), dissolved oxygen (4.8 mg/L), pH (12.08), nitratenitrogen (3.29 mg/L), phosphate-phosphorus (1.97 mg/L), and nitrite-nitrogen (0.063 mg/L) were observed in optimum levels.

Keywords: Culture, Spirulina, Papaya skin powder

Location: Department of Fisheries Management, Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh

Start Date: 00-06-2002

End Date: 00-08-2002

Program Area: Resource Development and Management

Commodity: Papaya

Objectives:

The present work was undertaken to study the growth performance of S. platensis in various concentrations of papaya skin powder.

Methodology:

The experiment was conducted for three months from June to August 2002 in the wet laboratory of the Department of Fisheries Management, Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. Papaya skins were collected from the Kamal-Ranjit market of BAU, Mymensingh and were dried in an oven at 50°C for over night. For complete drying, these papaya skins were dried under sun for another seven days. Then Hamam Dista was used to make powder of the dried papaya skins. For getting very fine particle of papaya skin powder, it was sieved through a siever of 500µm mesh size. Collection of Spirulina platensis Spirulina platensis under Order-Volvocales, Class-Chlorophyceae was collected from the laboratory of the Department of Aquaculture, BAU, Mymensingh. Pure stock culture maintenance • For purity, the stock culture of S. platensis was maintained in the laboratory in Kosaric medium (KM). Growth of S. platensis was monitored and it was then checked under microscope to confirm its purity. Preparation of papaya skin powder medium (PSPM) and Kosaric medium (KM) for S. platensis culture For preparation of papaya skin powder medium, 0.3, 0.4 and 0.5g of papaya skin powder were taken into a 1.0 litre conical flask with three replications. Distilled water was added into the conical flask to dilute it and made 1.0 L volume. About 400 ml was taken in each 1.0 L flask. Then the medium was mixed well and sterilized at 120°C for 15 minutes with moist heat autoclave (Express Equipment, Dixon's Surgical Instrument LTD.). Stock solutions of different chemical ingredients of KM (Phang and Chu, 1999) were prepared with distilled water and kept in freezer. The KM was prepared by taking required amount of solution from each stock in 1.0 L conical flask and volume was made up to one litre marked with distilled water. Then mixing, sterilization and cooling of the prepared media were done as followed the same procedure during the PSPM preparation. Culture of S. platensis in papaya skin powder medium At first, S. platensis were inoculated into three one litre culture bottle containing 0.3, 0.4 and 0.5 g/L of papaya skin powder medium respectively to produce a culture containing 10% S. platensis suspension (Optical density at 620 nm . 0.41) (Habib, 1998). 37.5 ml/L of S. platensis suspension was required for getting the density. Similarly, same inocula were taken into four culture bottles containing various concentrations (0.3, 0.4 and 0.5 g/L) of PSPM and KM. All the bottles were kept under fluorescent light (TFC, FL-40SD/38 Day light, Taiwan) in light: dark (12:12) conditions in the laboratory. These cultured bottles were continuously aerated using electric aerator (Davio Pump, Aquarium Pump NS-8200). Seven sub samplings were taken at every alternative day from each bottle to observe S. platensis cell density, chlorophyll a, water quality parameters (physical and chemical parameters) of culture media. All the glassware's used in the experiment were sterilized by dry heat in an oven at 70°C. Experimental design of S. platensis culture The two types of media viz. PSPM and KM were used for the culture of S. platensis. For PSPM 3 treatments each of 3 replications and for KM 1 treatment of 3 replications were used. For both the culture media 12 days culture period was maintained. Inoculum of S. platensis was collected from the pure stock culture. Estimation of S. platensis cell weight (g/L) For cell weight 10 ml of S. platensis sample from each treatment was taken and filtered with an electric filtration unit using filter papers (Whatman. GF/C) and shifted to the oven at 105°C for 24 hours. The samples were then transferred to the dessicator for coiling and weight was measured using an electric balance. Before filtering the weight of the filter paper was taken.

Source of Information: J. Bangladesh Agril. Univ. 5(1): 117-128, 2007 ISSN 1810-3030

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Funding Source:

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Achievement/Findings:

The result indicated that the growth rate of S. platensis was significantly (p<0.01) higher in 0.3 g/L concentration of PSPM than other concentrations of PSPM. The physico-chemical parameters viz., temperature (30.06 °C), light intensity (2110 lux/m2/s), dissolved oxygen (4.8 mg/L), pH (12.08), nitratenitrogen (3.29 mg/L), phosphate-phosphorus (1.97 mg/L), and nitrite-nitrogen (0.063 mg/L) were observed in optimum levels.

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