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In Vitro Regeneration of Bitter Gourd (momordica Charantia) (2016-2017)

M. K. Hasan
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. K. Jamil
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

S. C. Halder
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

D. Khnam
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

For the development of a suitable regeneration protocol of bitter gourd, leaf segments of BARI Korala-1 and Tia were cultured on MS medium supplemented with different concentrations of plant growth regulators. The types of responses were different in various plant growth regulators. Among the treatments, 1.5 and 2.0 ml/l 2, 4-D treatments found better for the callus formation.

Keywords: Bitter gourd, Tia, BARI Corala-1, Distilled water, Air flow cabinet, BARI Bitter gourd, Seed

Location: Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur and ‘Tia’ was collected from Lal Teer seed company, Bangladesh

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Crop-Soil-Water Management

Commodity: Bitter gourd

Objectives:

To determine of a suitable regeneration protocol of bitter gourd of BARI Korala-1 and Tia.

Methodology:

BARI Bitter gourd 1 seeds were collected from Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur and ‘Tia’ was collected from Lal Teer seed company. Seeds were sown in soil. After germination, young leaves were sterilized with Clorox solution (60 ml Clorox: 40 ml distilled water) with 2 drops of Tween-20 for 5-6min followed by 3-4 times washing with sterile distilled water then 70 % Ethanol for 30 second followed by 3-4 times washing with sterile distilled water inside the laminar air flow cabinet. The explants were cut off to leave 2-5 mm. The prepared explants were placed in MS media (Murashige and Skoog, 1962) with different concentrations and combinations of 2, 4-D. All cultures were incubated at 25 ± 1?C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes for three weeks.

Source of Information: Annual Research Report 2016-2017, Biotechnology Division, BARI, Joydebpur, Gazipur-1701, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The types of responses were different in various plant growth regulators. Among the treatments, 1.5 and 2.0 ml/l 2, 4-D treatments found better for the callus formation. Among the treatments, 1.5 and 2.0 ml/l 2, 4-D treatments found better for the callus formation. The experiment will be continued in the next year.

Knowledge Management: Report/Proceedings