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Large Scale Production of BARI Released Banana Through Tissue Culture (2016-2017)

S. C. Halder
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

S. Islam
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

D. Khanam
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

Sword suckers of a plantain variety BARI Kala-5 were collected from the farm field of Pomology Division, HRC. This line will be released within the short period. Due to its better performance than the previously released BARI Kala -2 variety the Pomology Division has a demand to increase its planting materials. That’s why; this line was taken under consideration this year for multiplication. Shoot tips were separated from those suckers and cultured on existing protocol based medium for in vitro production. Due to unavailable supply of sword suckers of the advanced line only 30 shoot tips were cultured on the shooting medium.

Keywords: Banana, Tissue culture, Micropropagation, BARI Kala -2, Vitro production

Location: Pomology Division of HRC, BARI, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Crop-Soil-Water Management

Commodity: Banana

Objectives:

To produce large scale banana plantlet through in vitro micropropagation.

Methodology:

Sword suckers of a plantain variety BARI Kala-5 were collected from Pomology Division of HRC. Shoot tips were separated from those suckers and cultured on MS (Murashige and Skoog, 1962) medium supplemented with 5 mg/l BAP . The inoculated cultures were incubated at 25±1⁰C under approximately 2500-3000 lux by cool white fluorescent tubes for 16/8 h light/dark cycle in 60-70% relative humidity. Data on percentage of success, bud initiation, no. of shoots /culture, length of shoots, days to root initiation, no. of root and length of roots/plant etc. were recorded. Performance of this line at different concentrations of different media will be study on availability of suckers.

Source of Information: Annual Research Report 2016-2017, Biotechnology Division, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Banana is a high value fruit crop. It has a great demand to the consumer level. For its well distribution micropropagation may play a pivotal. This experiment will be continued for obtaining new achievement in banana production.

Knowledge Management: Report/Proceedings