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First Record of Roving Coral Grouper Plectropomus Pessuliferus (fowler, 1904) from Saint Martin’s Island, Bangladesh Based on Morphology and DNA BARCoding

Md Jayedul Islam
Aquatic Bioresource Research Lab (ABR Lab), Department of Fisheries Biology and Genetics Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Sharmin Akter
Aquatic Bioresource Research Lab (ABR Lab), Department of Fisheries Biology and Genetics Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Provakor Sarkar
Aquatic Bioresource Research Lab (ABR Lab), Department of Fisheries Biology and Genetics Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Mohammad Rashed
Department of Fisheries Biology and Genetics, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Ireen Parvin
Department of Fisheries Biology and Genetics, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Kazi Ahsan Habib
Department of Fisheries Biology and Genetics, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh

Abstract/ Executive Summary:

A new record of Plectropomus pessuliferus (Serranidae: Epinephelinae) was documented based on morphological characters and DNA barcoding. The species was collected during a regular survey for making an inventory of reef associated fishes in Saint Martin`s Island, Bangladesh. This is the first report of roving coral grouper from the marine waters of Bangladesh validated by morpho-meristic analysis and DNA barcoding. This is also the first report from the northern Bay of Bengal.

Keywords: Plectropomus pessuliferus, Bay of Bengal, Epinephelinae, DNA barcoding

Location: The Aquatic Bioresource Research Lab., Department of Fisheries Biology and Genetics, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Start Date: 00-01-2018

End Date: 00-12-2018

Program Area: Animal Health and Management

Commodity: Aquatic animal

Objectives:

To identification of Plectropomus pessuliferus to carried out observing morphological characters.

Methodology:

Sample collection: In March 2018, one individual of Plectropomus pessuliferus was collected during a survey on the diversity of coral associated fish from Saint Martin’s Island, the southernmost part of Bangladesh facing the Bay of Bengal and located in Cox’s Bazar district. Morphological analysis: For morphological identification, taxonomic keys used by Randall and Heemstra (1991), Heemstra and Randall (1993), Baldwin and Johnson (1994) and Elamin et al. (2011) were followed. Abbreviations: D1- First dorsal fin, P1- Pectoral fin, P2- Pelvic fin, A-Anal fin, C- Caudal fin. Molecular study: For molecular study, a piece of muscle tissue was collected and preserved in 95% ethanol. Genomic DNA was extracted from preserved tissue sample using TIANamp Marine Animals DNA Kit (TIANGEN). The DNA barcode region of mitochondrial COI gene was amplified using the primer pair F1: 5’-TCAACCAACCACAAAGACATTGGCAC-3’ and R1: 5’-TAGACTTCTGG GTGGCCAAAGAATCA-3’ (Ward et al. 2005). Polymerase chain reaction (PCR) was performed in a 50µl reaction mixture in a thermal cycler (2720 Thermal Cycler, Applied Biosystems). Thermal cycling conditions included an initial denaturation temperature of 95oC for 2 minutes and subsequently, 35 cycles consisting 94^oC for 40 seconds for denaturation, 54^oC for 40 seconds for annealing and 72°C for 1 min for extension. Lastly, 72 °C for 10 min was maintained for final extension. PCR products were examined by 1% agarose gel electrophoresis with a 100 bp DNA ladder. Sequencing was conducted with the PCR primers by Sanger standard method in a normal automatic sequencer (Model 3730xI DNA analyzer. For molecular identification, we checked the DNA sequence using BLAST search engine provided by the National Center for Biotechnology Information (NCBI) and BOLD database. Finally, the obtained nucleotide sequence of COI barcode region was submitted to GenBank under the accession number of MK340678. For phylogenetic analysis, including the sequence of present study we used a total of five sequences of three species of the genus Plectropomus (i.e. P. pessuliferus, P. leopardus, P. maculatus) obtained from the BLAST search result in GenBank (Altschul et al. 1990). The Kimura-2 parameter (K2P) distance model (Kimura 1980) was used for calculating the genetic distance among the sequences using MEGA7. Aconfamiliar species Cephalopholis boenak (Bloch 1790) was used as an outgroup. Sequences were aligned and phylogenetic tree was constructed by Neighbor Joining (NJ) method using MEGA7 (Kumar et al., 2016). Robustness of the phylogenetic relationships was evaluated by bootstrap analysis with 10,000 replications (Felsenstein 1985). The examined specimen was deposited in the Aquatic Bioresource Research Lab., Department of Fisheries Biology and Genetics, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.

Source of Information: Bangladesh J. Fish. (2020) 32(2) : 293-301

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The nearby areas of the Bay of Bengal from where the species P. pessuliferus was known to occur are Andaman and Nicobar Island, Srilanka and Myanmar but these species has no valid record from the northern part of the Bay of Bengal. This study confirms the presence of P. pessuliferus in the Saint Martin’s Island of the north Bay of Bengal and indicates the possibility of existence of more species of the genus Plectropomus in Bangladeshi marine water that have been overlooked in past surveys. This is the first report of roving coral grouper from the marine waters of Bangladesh validated by morpho-meristic analysis and DNA barcoding. This is also the first report from the northern Bay of Bengal.

Knowledge Management: Journal