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Antimicrobial Activity of Methanolic Extract of Langra Mango Pulp

Amit Sarker
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Nurul Amin
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Israt Jahan Shimu
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Md. Parvez Akhter
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Md. Ashickul Alam
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Md. Mizanur Rahman
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Habiba Sultana
Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh

Abstract/ Executive Summary:

Mango is antioxidant rich one of the most popular fruits in the world. Mangoes (Mangifera indica) belong to genus Mangifera under the family of Anacardiaceae. In Bangladesh, Mango is called as the “King of fruit” over a large area, but locally known as “aam”. Mango contains different types of chemicals and most of them are bioactive compounds such as polyphenols, vitamin C, carotenoids, anthocyanin, and flavonoids. The present study was to extract the chemicals from Langra mango as well as investigated the antimicrobial properties by using various in vitro methods. The mature fruit was extracted with methanol. Chemicals from mango pulps were extracted by using Rotary evaporator. Phytochemical tests were performed to investigate the possible phytochemicals present in the mango pulp extract. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were performed by disc diffusion and broth dilution assay against five (5) pathogenic bacteria. The antimicrobial activity exerted by the extract was compared with standard and results showed that extract contained significant antimicrobial activity. The order of zone of inhibition was Staphylococcus aureus (17mm) > Bacillus subtilis (16 mm) > Escherichia coli (14 mm) > Salmonella typhi (14 mm) > Klebsiella pneumoniae (12 mm). The MIC values were in the range 0.6 mg/ml to 1.25 mg/ml for extract of Langra mango pulp. The lowest MIC was found for Escherichia coli (0.6 mg/ml). So the extract (methanolic) of langra mango pulp has potent antimicrobial properties.

Keywords: Langra mango pulp, Antimicrobial activity, Minimum Inhibitory Concentration (MIC), Pathogenic bacteria

Location: Mohakhali Kacha bazar, Dhaka, Bangladesh

Start Date: 00-05-2016

End Date:

Program Area: Pest Management

Commodity: Mango

Objectives:

Hence the major objective of the present work was to evaluate the chemical composition, antibacterial effect activity of ripe mango pulp extract.

Methodology:

According to the investigational procedure the mature fruit mango were collected from Mohakhali Kacha bazar, Dhaka, Bangladesh in the month of May, 2016. Preparation of extract The pulp parts of the collected mango fruits were shade dried for seven days.After drying pulp (50 gm) were dissolved in methanol (250 ml methanol) for five days in a glass container (appropriate labeling) with occasional shaking. The mixtures were filtered through a plug of cotton and separate the excess solvent by using rotary evaporator. After separation remaining portion was used as a sample. Microorganisms Pure culture of Escherichia Coli, Bacillus subtilis, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were obtained from the Microbiology Laboratory of Department of Pharmacy, Primeasia University, Dhaka, Bangladesh (106 CFU ml-1). Phytochemical Screening Carbohydrates, proteins, alkaloids, glycosides, anthraquinone glycosides, tannins, saponins, flavonoids and resins were detected by standard procedures. Different reagents were (Molishch’s reagents (10% naphthol in alcohol) - for carbohydrate test, Aqueous sodium hydroxide solution- for glycoside test, Dilute sulphuric acid and NaOH solution- for glycoside test, Fehling’s solution- for glycoside test, 10% Ammonia solution- for anthraquinone glycoside test, Mayer’s reagent (potassiomercuric iodide solution), Wagner’s reagent (solution of I in KI), Hager’s reagent (Saturated solution of picric acid), Dragendroff’s reagent (Bismuth sub nitrate and acetic acid solution)- All for alkaloid tests, Conc. Hydroclric acid – for flavanoid test, Conc. Sulphuric acid- for steroid test, FeCl3 (5%) - for tannin test ) used for this purposes. Antimicrobial Activity Antimicrobial activity of the fruits extracts (Langra mango) was tested by disc diffusion method. The extract solution was prepared at a concentration of 100 μg/disc. Then extract was infused into the paper discs and placed onto the nutrient agar media inoculated with the test bacteria and incubated at 37^o C for 24 h. Kanamycin (30 μg/disc) (oxoid, UK) was used as standard. After incubation, zone of inhibition formed around the disc was examined and assessing the antimicrobial activity on the basis of measurement of diameter of the inhibited zone formed around the disc. Minimum Inhibitory Concentration (MIC) Determination of the Minimum Inhibitory Concentration (MIC) was carried using the broth dilution method. To obtain a concentration 100 mg/ml, 50µl/4ml of the reconstituted extract solution at a concentration of 10mg/ml was added to test tube containing 1 ml of sterile broth. 1 ml of this dilution was transferred to another test tube till the 10th test tube was reached. The 11th test tube did not contain any extract, but a solution of pure solvent served as negative control. Then 1 ml of an 18 h old culture of each of the organisms earlier adjusted at 106 CFU/ml was put into each tube and thoroughly vortexes. The tubes were incubated at 37^o C for 24 h and observed the growth of bacteria in form of turbidity. The test tube with the lowest dilution with no detectable growth by visual inspection was considered the MIC.

Source of Information: Journal of Pharmacognosy and Phytochemistry 2017; 6(6): 28-30

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The findings of our present work emphasizing on the antimicrobial activity of the pulp of Langra mango shows significant activity against five microorganisms. This work can be authenticated by isolating and exploring the responsible chemical constituents for the given activities. This could lead to the discovery of some novel molecules in this particular field of interest.

Knowledge Management: Journal