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A Study on in Vitro Interaction of Cephradine with Mango Juice at Lower Ph

Jony Mallik*
Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh

Abstract/ Executive Summary:

Cephradine is a first-generation semi-synthetic cephalosporin antibiotic, is widely used in clinics for its activity against both Gram-positive and Gram-negative bacteria. It is indicated for the treatment of urinary tract infections, skin and skin structure infections, respiratory tract infections and otitis media. Interactions of cephradine with mango juice were investigated by UV-spectrophotometer in simulated gastric juice (pH 1.2&3.2). In this research work, Cephradine capsules were collected from drug shops. The samples were analyzed according to British Pharmacopoeia (BP) method 3. In this work in vitro dissolution studies were carried out in 900ml of acidic buffer (pH 1.2&3.2) in a dissolution tester with a speed of 51 rpm at 37±0.5 ⁰C for six hours. The absorbance was measured by using UV - spectrophotometer at a λmax of 254nm. Compare with the absorbance of the drug and the drug in presence of mango juice simulated gastric juice (pH 1.2&3.2). The drug release kinetics was also measured. It is observed from the drug release profile that, there is no significant difference in the drug release curve of pH 1.2&3.2.

Keywords: Cephradine, UV-spectrophotometer, Dissolution, Mango juice.

Location: Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Mango, Juice

Objectives:

We report a case of severe anaphylactic reaction to Cephradine in an elderly patient who had no history of allergic reactions to any drugs until then.

Methodology:

Feature of Cephradine standard Cephradine (compacted powder) Potency: 99.14% LOD- 4 %( NMT) Origin: China Collected from: Pharmik Laboratories Ltd. Reagents used in this work 1. Hydrochloric acid, 2. Sodium hydroxide, 3. Citric acid, 4. Pottasium chloride, 5. Di-sodium hydrogen orthophosphate, 6. Potassium di-hydrogen orthophosphate. They were all of analytical grade. One brands of cephradine capsule and the innovator brands with labeled contents of 500mg each were obtained from retail pharmacies in Chittagong city. The samples were checked for their production and expiry dates before purchasing. Instrument Used in this Method 1. Electric balance 2. UV- Spectrophotometer 3. Dissolution Apparatus 4. Thermometer 5. pH Meter DISSOLUTION RATE DETERMINATION This was determined using the Pharma Test Dissolution Rate Testing Apparatus (Model D63512, Hainburg). These studies were conducted at 37±0.50 ⁰C on a USP specification dissolution rate test type II apparatus (Paddle apparatus) with six section assembly according to the USP XXIII procedure with minor modification (USP XXII and NF XVII, 1995). For in vitro dissolution studies simulated gastric medium (pH 1.2 & 3.2) and simulated intestinal medium (pH 6.8) was required. Preparation of simulated gastric medium (pH 1.2) 1000ml buffer solution with pH 1.2 250 ml of 0.1 M HCl solution was taken in 1000ml beaker and 500ml 0.1M HCl solution were added into the 1000ml beaker. Adjust the pH 1.2 with adding distilled & demineralised water respectively. After adjusting the pH of the buffer solution the buffer solution was taken into1000ml volumetric flask. Preparation of simulated gastric medium (pH 3.2) 1000ml buffer solution with pH 3.2 250 ml of 0.1 M HCl solution was taken in 1000ml beaker and 500ml 0.1M HCl solution were added into the 1000ml beaker. Adjust the pH 3.2 with adding distilled & demineralized water respectively. After adjusting the pH of the buffer solution the buffer solution was taken into1000ml volumetric flask. Dissolution study statement The dissolution study of Cephradine(Lebac®) was investigated firstly in the presence of tap water and then same investigation was performed in the presence of buffer solution pH 1.2 prepared by using both distilled and demineralized water. The dissolution study was investigated in the presence of buffer solution pH 3.2 prepared by using demineralized water. The dissolution study of Cephradine(Lebac) was investigated in the presence of 250ml mango juice(Pran Frooto) and 650ml of buffer solution pH 1.2. Again dissolution studies of Cephradine (Lebac®) were investigated in the presence of 250ml mango juice 650ml of buffer solution pH 3.2. Dissolution study description According to the statement every time two capsules are places in the baskets, where one basket contain the the drug (cephradine capsule form) and another basket contain the drug with mango juice. First time drug was placed in tap water and demineralized water. Here one drug was placed in tap water and another was in demineralized water. Next time a drug was placed in buffer with pH 1.2 and another was in a combined solution of 250ml mango juice and 650ml buffer pH 1.2.again a drug was placed in buffer with pH 3.2 and another was in combined solution of 250ml mango juice and 650ml buffer pH 3.2.again. The operation in the acid stages were carried out for 6 hours.Than the dissolution apparatus are switched on and the temperature was 37ºC and the rpm was 51.At every time interval 5ml solution was taken into test tube and the volume adjust by fresh media.The time interval were followings:- 0min, 5min, 10min, 20min, 30min, 45min, 60min, 90min, 135min, 195min, 285,min, 360min (upto 6 hours). From the test tube of each,1ml was taken into 100ml volumetric flask and it is diluted to 100ml with buffer. Than it was filtered, taken into cell and the released drug was assayed by using UV spectrophotometer at 254nm.

Source of Information: International Journal of Pharmaceutical & Biological Archives 2012; 3(4):764-769

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Total Budget (Tk.) :

Achievement/Findings:

The percent release data suggest that, in simulated gastric medium (pH1.2 and 3.2), the percent release of Cephradine not increased significantly. It is also seen that in different pH the percent release neither increased nor decreased when Cephradine is taken with the Mango juice. From the Correlation coefficients determination data it is seen that, Correlation Coefficients (R2 1. Ameer B, Weintraub RA. Drug interactions with grapefruit juice. Clin Pharmacokinet 1997; 3:103-21. ) is close to 1 in case of Higuchi plot. So Higuchi release kinetics predominates in simulated gastric medium of pH 1.2 and 3.2. It is also observed from the release kinetics profile (Zero order, First order, Higuchi), both lines are close to each other and there is no significant distance between two lines. Both the lines appear in between 0-20 percent of drug release. Hence, we can reveal that, on the basis of our present study if the patients take Cephradine and Mango juice at a time, no harmful effect will occur.

Knowledge Management: Journal