Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Trial of Sex Reverse Percentage on Female Shing (heteropneustes Fossilis) by Using 17β-estradiol Sex Hormone

Gias Uddin Ahmed
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Mamunur Rahman
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Mohammad Nurul Alam
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University Mymensingh,-2202, Bangladesh

Md. Ashraful Alam
Microbiologst, Jahanabad Sea Foods Ltd., Khulna, Bangladesh.

Abstract/ Executive Summary:

A study was conducted to evaluate the production of monosex (female) shing ( Heteropneustes fossilis ) by using a sex hormone (17β-Estradiol). The experiment was composed of three treatments T₁ (70 mg 17β-Estradiol /kg feed), T₂ (80 mg 17β Estradiol /kg feed) and T₃ (90mg 17β- Estradiol /kg feed) in duplicate were fed shing fishes in cemented cistern. The hormone was administered orally at variable doses with Tiger brand nursery fish feed (Eon Group) for 30 days. The fry were fed with the hormone mixed feed five times in a day. Every seven days intervals 50% water of the tanks were changed. A high mortality rate (47.6% to 67.4%) was observed in different treatments. The occurrence of female fish at the end of the experiment in treatment TI, T₂ and T₃ were 75%, 86%, and 96% female respectively. T₃ (90 mg 17β-Estradiol /kg, feed) showed the best performance with 96% sex reversal to female progeny.

Keywords: Sex reverse, Heteropneustes fossilis, 17β-Estradiol, Sex hormone

Location: “Noha Aqua Farm and Hatchery” at Muktaghasa region of Mymensingh district, Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Aquatic animal

Objectives:

The objective of this research is to determine the optimal dose of estrogen hormone (17β-Estradiol) for the production of mono-sex female Heteropneustes fossilis.

Methodology:

Study area: The experiment was conducted in six 4m² rectangular cemented cisterns. The experiment was carried out in “Noha Aqua Farm and Hatchery” at Muktaghasa region of Mymensingh district.

Preparation of cisterns: Cisterns were cleaned with Active blue (anti-fungal) and potassium permanganate solution thoroughly and two times washes the cistern. Then the cisterns were filled with water. All the cisterns were maintained with a water level of 40 cm depth throughout the period of 33 days. Then the water level was maintained 75 cm depth. Each cistern was provided with pieces of PVC pipes (4cm diameter) as shelter for fish.

Spawning of Heteropneustes fossilis: Spawning of Heteropneustes fossilis was performed in the Noha Aqua Farm and Hatchery at Muktaghasa region of Mymensingh district. Brood fishes were collected from outside of this hatchery. Only good looking, conspicuous, healthy and uninjured fishes were selected for induced breeding. Sexes were determined by careful examination. Handling of fish was done very carefully to avoid injury and secondary infection. Human Chorionic Gonadotropin (HCG) was used to induce breeding and injected at 1000 IU/kg body weight of both male and female fishes. For all the treatments, the hormone was administered by intra-muscular injection on muscles beneath the dorsal fin slightly above the lateral line. After injection, the brooders were kept in separate breeding tanks for each treatment.

Experimental designs: After hatching 500 spawns were kept in tray (30 cm x 50 cm x 12 cm; 12.6 liter capacity) for 3 days. The experiment was designed with three treatments (T₁, T₂ and T₃) in two replication. Each replicate contained 500 fry. T₁, T₂ and T₃ were steroid treatment orally. Steroid (17β - Estradiol) treatment consisted of three doses (70 mg 17β -Estradiol /kg feed, 80 mg 17β - Estradiol /kg feed, 90 mg 17β- Estradiol /kg feed in duplicate were used.

Diet formulations: The experimental diet with different doses of 17β - Estradiol (i.e. 70 mg/kg feed, 80 mg/kg feed, 90 mg/kg feed) were prepared through ethanol evaporation method (Nair and Santiago, 1994). The experimental diet was used Tiger brand nursery fish feed (Eon Group) 55% protein for the sex reversal purpose.

Process of diet formulation and preservation: The required amount of hormones and feed were measured by electrical balance and ethanol was measured by measuring cylinder. At first 500 mg of 17β - Estradiol was diluted with 200 ml ethanol to prepare stock solution.

Starting of the experiment: After hatching the spawns were kept in 6 trays (30 cm x 50 cm x 12 cm) for 2 days. Then spawns were transfer from Noha Aqua Farm and Hatchery to the cisterns. The first feeding was starting at 48 hours later of hatching. The first feeding was hormone (17β -Estradiol) mixed Tiger brand nursery fish feed. The experiment was comprised of three treatments viz. T1 70 mg/kg feed, T2 80 mg/kg feed and T3 90 mg/kg feed doses of 17β –Estradiol.

Rearing of fry:The spawns were fed with the hormone mixed feed five times with three hour interval in a day. 50% of the water was changed every seven days interval. Faecal matter and unused feed was siphoned out every day just after feeding and replaced water was added. The hormone treatment was conducted for 30 days. During hormone treatment period, first 15 days they fed with 200% of their body weight and rest 15 days 100%.

Sex identification: The fishes were sexed by gonad squashing and aceto-carmine staining method (Guerreo and Shelton, 1974). The fish was killed and the viscera were removed. The gonads were lying along the surface of the body cavity on either side of the fish just above the kidney. The gonads were removed and placed on a clean glass slide A few drop of aceto-carmine stain were added and the gonads squashed with a cover slip. The slides were examining under a microscope (Novex Holland, K-RANGE; WF10 X, and SPL 10/0.25). Ovarian tissue was identified by the presence of scattered oocytes and the testicular tissue was identified by the presence of spermatocytes with a uniform background and the intermingling of spermatogonia and oocytes in the same gonad.

Source of Information: Res. Agric. Livest. Fish. Vol. 2, No. 2, August 2015: 313-317 ISSN : P-2409-0603, E-2409-9325

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The experiment was carried out in six rectangular cemented cisterns (4m² each) located in the Noha Aqua Farm and Hatchery at Muktaghasa region of Mymensingh. Estrogen hormone (17β -Estradiol) was administered orally at 3 different doses viz.70, 80 and 90 mg per kg with Tiger brand nursery fish feed were used for a period of 30 days (15th March to 13th April, 2015). Feeding was done 5 times with three hours interval in a day. The experiment was designed with three treatments viz. T1 (70 mg/kg Iced), T2 (80 mg/kg feed) and T3 (90 mg/kg feed) with two replications. For each treatment 500 fry were used. The experimental diet with different doses 17β -Estradiol (i.e. 70 mg, 80 mg and 90 mg / kg feed) was prepared through ethanol evaporation method (Mair and Santiago, 1994). The diets were preserved in dry and cold place 7 days and temperature maintain at 100 C. 50% of the water in the cisterns was changed every seven days interval prior to feeding, to remove fecal matter and unfed materials. After completion of the rearing, sex identification was done by dissecting the fish. A high rate of mortality was observed in all treatments. Average mortality rate ranged from 47.6% to 67.4%. Highest mortality was observed during the first 7 days. In case of feminization the average percentage of female in different treatments, TI, T2 and T3 were 75%, 86% and 96% respectively. T3 showed the best performance with 96% sex reversal to female progeny, which was significantly different from other treatments. Increasing frequency of female and decreasing number of males and ovotestes bearing Fish indicates that 100% female fish can be obtained by optimizing the hormone dose and the duration of the hormone treatment.

Knowledge Management: Journal