Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation, Identification and Antibiogram Study of Escherichia Coli from the Cases of Mastitis

Md. Majadur Rahman
Bangladesh University of Professional, Mirpur Cantonment, Dhaka, Bangladesh

Md. Fakhruzzaman
Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200

Md. Mostafizer Rahman
Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200

Rashida Khaton
Department of Veterinary and Animal Sciences, University of Rajshahi, Bangladesh

Shah Md. Iqbal
Department of Livestock Services, Ministry of Fishery and Livestock, Bangladesh

Shyla Sharmin
Department of Veterinary and Animal Sciences, University of Rajshahi, Bangladesh

Md. Hemayatul Islam
Department of Veterinary and Animal Sciences, University of Rajshahi, Bangladesh

Abstract/ Executive Summary:

Context: Mastitis is the devastating diseases among the dairy farms in Bangladesh. Objectives: To isolate, identify and antibiogram study of Escherichia coli from the cases of mastitis among suspected dairy farm. Materials and methods:The study was conducted to isolate, identify and antibiogram study of significant bacterial pathogens causing mastitis in dairy cow. The study was conducted on 50 suspected dairy cows from 5 commercial dairy farms and Upazilla veterinary hospitals during the period from March, 2011 to August, 2011 of Gaibandha district under the Department of Microbiology Hajee Mohammed Danesh Science and Technology University, Dinajpur, Bangladesh. Results: Result found on milk samples from different sources were 30% in positive for Escherichia coli, 20% were found to be positive for Staphylococcus species. 20% for Streptococcus species, 14% were Corynebacterium species and16% were undefined species of mixed bacteria. Results of the antibiograms among various isolates of Escherichia coli were found to be highly sensitive (+++) to Chloramphenicol (70%), Kanamycin (70%) and Nitrofurantoin (90%). Conclusion: Among the isolated bacterial pathogen Escherichia coli plays significant role (30%) on mastitis infection and it is highly sensitive to Nitrofurantoin, Chloramphenicol and Kanamycin. As Nitrofurantoin has a huge public health hazard, so drug of choice can be Chloramphenicol and Kanamycin against Escherichia coli.

Keywords: Mastitis, Bacterial pathogen, Dairy farm, Antibiogram, Drug of choice

Location: The department of Microbiology Hajee Mohammed Danesh Science and Technology University, Dinajpur, Bangladesh

Start Date: 00-03-2011

End Date: 00-08-2011

Program Area: Animal Health and Management

Commodity: Diseases

Objectives:

The objectives:

♦ To study the prevalence of Clinical and sub clinical mastitis of cow in Gaibandha district

♦ To isolate and identify the Escherichia coli associated with Clinical and sub clinical mastitis in cow.

Methodology:

The study was carried out during the period from March, 2011 to August, 2011 in the department of Microbiology Hajee Mohammed Danesh Science and Technology University, Dinajpur, Bangladesh. The detailed outline of the materials and methods are given below: Husbandry and management system: The cows are managed under intensive husbandry system with semi paca floor in dairy farm and in local farms the animals are reared by the traditional system. They are often provided with some green grass in addition to natural pasture and concentrate diet and are kept together in common shed. In local farms, they are gazed at day time and kept in kacha floor as in traditional housing system. Survey design and sampling: A cross-sectional observational study was conducted in crossbreed dairy farm, indigenous lactating cow and various dairy cows of different house in Palashbari, Sadullapur, Gobindogonj Upazila of Gaibandha district. Selection of experimental animals: Clinically affected and subclinically suspected Mastitis cow was selected from the commercial dairy farm, Veterinary hospital and lactating cow of different farmer of the study area were selected for the study. Samples: collected from healthy and infected teat from the diseased cow. Samples were also collected from apparently healthy dairy cow. Collection of milk sample Milk samples were collected by the soaking the teat with Povidone Iodine and drying off by tissue paper, one to two drops of milk was discarded and then 2-3 ml of milk was taken from each quarter into labeled sterilized test tubes with rubber cap\screw cap or cotton Plaque. Results and Discussions: A total of 50 milk samples were collected from different area of Gaibandha district (Table 01) in sterilized test tube following aseptic measures. Out of 50 milk samples 29 were collected from clinically mastitis infected cows, 11 milk samples were collected from sub-clinically suspected cows and the rest 10 milk samples were collected from healthy cows. Of the samples tested, 18.04% and 22.03% animals, and 15.05% and 16.81% quarter wise were found to be positive for subclinical mastitis by using California Mastitis Test (CMT) and Somatic Cell Count (SCC) respectively. Prevalence was found by CMT compared to SCC. The CMT result was somewhat lower than the report of Schmidt et al.,(2009) who reported 22.50%. The result of Somatic Cell Count (SCC) revealed the prevalence of sub-clinical mastitis 16.81% in the present study which somewhat lower than the results of Min et al., (2007) who reported 19-30% positive result. The highest prevalence (80.00%) of sub-clinical mastitis was recorded at 2-3 years aged group and lowest prevalence (6.25%) of SCM at 5-6 years aged group. The highest prevalence (66.66%) of SCM was found at lactation whereas the lowest prevalence (4.76%) of SCM at the 5th or 6th lactation period. These findings support the report of Neelesh-Sharma et al., (2007) who reported the highest prevalence of SCM during the 1st lactation and at 2 to 3 years of age in cows. An obvious trend of the increasing prevalence of SCM to the stage of lactation observed in this study. Highest prevalence (50.00%) of SCM was found at early lactation stage whereas lowest prevalence (23.68%) of SCM was found at mid-lactation stage (4-8 weeks). These observations were also supported by the report of NeeleshSharma et al., (2007) who observed the infection rate higher during the 1st lactation followed by early and mid-stage of lactation. The bacteria isolated from the positive cases in this study were Escherichia coli. Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Gram-negative rods. Aydn et al., (2007) and Kostelic et al.,(2009) also isolated these bacteria from milk samples originating from sub clinical mastitis of cow. Among 50 milk samples, 15 (30.00%) were found positive for Escherichia coli, 10 (20.00 %) were found to be positive for Staphylococcus species., 10(20.00%) for Streptococcus species, 7 (14.00%) for Corynaebacterium species, and8 (16.00%) were undefined species. of mixed bacteria. Cultural examination revealed that Staphylococcus species as the predominant organisms followed by other bacteria. These findings are in agreement with the earlier report that made by Mhase et al., (2007) in which they reported Staphylococcus aureus as the highest (34.38%) frequency, followed by Bacillus in12.50 % Escherichia coli (25.00%) in cow.

Source of Information: Bangladesh livestock journal. 2018. 1: 1-6; ISSN 2409-7691

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Nutrient broth: The organisms of the milk sample produced turbidity in nutrient broth. Nutrient agar: After incubation on nutrient agar organisms were produced circular, smooth and colorless colonies. Salmonella-Shigella agar: The organisms of the milk sample produced slight pinkish smooth colonies were tentatively choosen to be Escherichia coli. MacConkey (MC) agar: After overnight incubation on MacConkey agar suspected organisms were produced bright pink colonies. Eosine methylene blue (EMB) agar: The greenish-black colonies with metallic sheen on Eosine methylene blue (EMB) agar were presumptively selected Escherichia coli. Staphylococcus 110 agar medium: Escherichia coli did not produce colonies where as staphylococcus produce whitish colonies. Results of Gram’s stain: In Gram’s staining under microscope the organisms revealed Gram-negative, pink color, small rod shaped arranged as single or paired. Successfully isolate the Escherichia coli from mastitis infected milk and find out the drug of choice for the treatment against Escherichia coli infected cases.

Knowledge Management: Journal