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Smart Production of Spirulina (spirulina Platensis) Using Supernatant of Digested Rotten Potato (solanum Tuberosum)

M. A. A. Hossain
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202

M. H. Rahman
Department of Aquaculture, Khulna Agricultural University, Khulna-9100

M. S. Hossain
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202

M. A. B. Habib
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202

M. A. Uddin
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

F. Sarker
Department of Life Science, University of Tasmania, Hobart-7005, Australia

Abstract/ Executive Summary:

The study was conducted to evaluate the culture and growth performance of spirulina (Spirulina platensis) in supernatant of three different amounts of digested rotten potato (DRP), and Kosaric medium (KM) as control in 16 days after 26 days digestion. Three different concentrations such as 20, 40 and 60% of DRP were used. Spirulina was inoculated in supernatant DRP along with 9.0 g/L NaHCO₃ and micronutrients, and KM for a period of 14 days. The cell weight of spirulina has attained a maximum of 12.42 ± 0.21 mg/L in KM followed by 8.352 ± 0.21, 6.256 ± 2.34 and 9.505 ± 0.43 mg/L in supernatant of 40, 20 and 60% DRP, respectively on the 10th day of culture. Similar trend was also observed in the cases of optical density, chlorophyll a, total biomass, specific growth rates. Cell weight of spirulina grown in the media were highly significant (p<0.01) and correlated with the chlorophyll a content and total biomass. The growth performance of spirulina grown in supernatant of 60% DRP was significantly higher than that of spirulina grown in supernatant of 20 and 40% DRP. Therefore, mass culture of spirulina may be done in supernatant of 60% DRP.

Keywords: Spirulina, Potato, Growth, Kosaric medium, Optical density

Location: The Laboratory of Fish Nutrition, Department of Aquaculture, Water Quality Laboratory within the Department of Fisheries Management and Genetics Laboratory in the Department of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, Bangladesh

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Potato, Fish, Feed

Objectives:

To take a look at the smart production of spirulina by means of supernatant of digested rotten potato (Solenum tuberosum) to measure the growth performances of S. platensis in 3 exclusive concentration of supernatant of digested rotten potato.

Methodology:

Study area: The study was performed inside the Laboratory of Fish Nutrition, Department of Aquaculture, Water Quality Laboratory within the Department of Fisheries Management and Genetics Laboratory in the Department of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202. Collection of rotten potato: The rotten potato become selected as medium for S. platensis culture because of presence of excessive natural in addition to inorganic vitamins specifically carbohydrate. The rotten potato becomes accrued from Seshmore market, BAU, Mymensigh. It became reduce into portions and used to digest in cardio circumstance, some element become dried, floor, packed in polythene bag and kept in the laboratory for future use. Collection of spirulina (S. platensis): Microalgae Spirulina platensis was collected from the stock of the laboratory of Live Food Culture department of Aquaculture, BAU, Mymensingh. For obtaining pure culture of spirulina maintained hygiene stock Torzillo et al., (1986). Maintenance of pure stock culture of spirulina: Pure stock culture of S. platensis was maintained in the laboratory in Kosaric Medium (KM) (Modified after Zarrouk, 1996). Growth of S. platensis was monitored at every alternative day and was checked under microscope to confirm its purity following keys of Bold and Wynne (1978), Vymazal (1995) and Phang and Chu (1999). Preparation of supernatant of DRP and Kosaric Medium (KM): 400 g/4.0 L wet rotten potato was allowed to decompose in 5.0 L glass bottle for 26 days under aerobic condition (Plate 1) in the laboratory of Animal Nutrition, BAU, Mymensingh. Then a Light reddish white coloured supernatant from bottle was screened through a net of 30 µm, mixed with 9.0 g/L sodium bicarbonate and 0.20 ml/L micronutrient, then diluted and made three concentrations at the rate of 20, 40 and 60% of decomposed rotten potato. Then the supernatant of three different concentrations were taken in 2.0 L flask with three replications. Simultaneously, Kosaric medium (KM) was prepared for S. platensis culture as a control. Then the medium in flasks have been blended properly and sterilized at 120°C for 15 minutes for 15 minutes with wet warmth with the aid of autoclave. After autoclaving, the media were saved for 24 hours to make certain approximately any infection loose earlier than lifestyle of microalgae. Specific growth rate (µ/day) of cultured spirulina on the basis of dry weight SGR (µ/day) = In (X₁-X₂)/t₁-t₂ Where, X₁ = Dry weight of biomass concentration of the end of selected time interval; X₂ = Dry weight biomass concentration at beginning of selected time interval; and t₁-t₂ = Elapsed time between selected time in the day. Specific growth rate (µ/day) of cultured spirulina on the basis of chlorophyll a SGR (µ/day) = In (X₁-X₂)/t₁-t₂ Where, X₁ = Chlorophyll a at the end of selected time interval; X₂ = Chlorophyll a at the beginning of selected time interval; and t₁-t₂ = Elapsed time between selected time in the day. Specific growth rate (µ/day) of cultured spirulina on the basis of total biomass SGR (µ/day) = In (X₁-X₂)/t₁-t₂ Where, X₁ = Total biomass at the end of selected time interval; X₂ = Total biomass at the beginning of selected time interval; and t₁-t₂ = Elapsed time between selected time in the day. Statistical analysis: Analysis of variance (ANOVA) of imply cell weight and chlorophyll a of S. platensis cultured in exclusive media had been carried out. To locate where there’s any great distinction among treatment means turned into performed by means of Duncan’s multiple range take a look at (DMRT) the usage of statistical bundle following Zar (1984).

Source of Information: http://journal.safebd.org/index.php/jafe; Vol 2 No 1 March 2021 Pages 62-69 e-ISSN 2708-5694

Article Link (Online Journal): http://doi.org/10.47440/JAFE.2021.2111

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

This research was performed on culture and growth performance of S. platensis in different concentrations of supernatant of digested rotten potato and KM in which growth is highest. S. platensis was cultured in supernatant of various concentration viz., 20, 40 and 60% digested rotten potato, and KM with three replications for each treatment under fluorescent light in light: dark (12hr: 12hr) condition for period of 14th days. Rotten potato may be used to grow spirulina due to presence of organic carbon as carbohydrate in potato. Spirulina grows well in supernatant of 60% digested rotten potato which is equivalent to the growth of spirulina in Kosaric medium. So, the supernatant of 60% digested rotten potato should be used to grow spirulina. The environment may be free from pollution due to use of rotten potatoes. So, there is a huge chance of large-scale rotten potato may be used to commercial culture of spirulina and marketed as live food for the good production and management of fish health.

Knowledge Management: Journal