Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Tissue Culture of Artocarpus Heterophyllus L., an Underutilized Fruit of Bangladesh

F.M. Safiul Azam
Department of Biotechnology & Genetic Engineering University of Development Alternative Dhanmondi, Dhaka, Bangladesh

Mohammed Rahmatullah
Department of Biotechnology & Genetic Engineering University of Development Alternative Dhanmondi, Dhaka, Bangladesh

Ather-uz-Zaman
Centre for Plant Tissue Culture, PROSHIKA, Dhaka, Bangladesh

Abstract/ Executive Summary:

Jackfruit (Artocarpus heterophyllus) is available in Bangladesh between June to August; however, a variety exists that fruits throughout the year. The edible portion is considered a good source of carbohydrates, proteins, vitamins and minerals. In Bangladesh, malnutrition persists among 35-45% of the population and the child malnutrition rate is more than 50%. Under this circumstance, the wide cultivation of a Jackfruit variety that bears fruit throughout the year can play a significant role in reducing malnutrition in Bangladesh. Since this particular variety is not widely available, we have developed a method for its clonal propagation. Healthy and juvenile shoot tips and nodal segments were collected from field-grown fruit-bearing trees and cultured in Murashige-Skoog (MS) media fortified with different concentrations and a combination of 6-benzyl amino purine and Kinetin following the sterilization with 0.1% HgCl2. Sprouting and regeneration of micro shoots geared up when MS was enriched with 3.5 mg/l BAP. Proliferation frequency increased considerably and multiple shoots were regenerated as a clump of 2-3 shoots in pH 5.8 media within four weeks of inoculation through the synergistic effect of 6-benzyl amino purine (3.5 mg/l) and Kinetin (1.5 mg/l). With the increase of subculture (up to the 10th maximum), the frequency of shoot proliferation was enhanced. The addition of 0.1 mg/l Indole-3-acetic acid and 20% coconut water considerably increased shoot elongation and stimulated the growth of the shoots. About 80% rooting frequency was observed in MS medium with 1.2 mg/l Indole-3- butyric acid. Rooting percentage and their growth was much better in liquid media. After proper acclimatization, rooted plantlets were transferred to polythene bags containing garden soil, sand and cowdung (1:1:1). After eight weeks of transplantation in an open field, more than 80% of plants were survived. No morphological variants were observed during the passage of clonal propagation. This clonal propagation technique could play a significant role in improving productivity leading to reduced malnutrition.

Keywords: Jackfruit, Clonal propagation, Synergistic effect, Malnutrition improvement

Location: Plant Tissue Culture Laboratory of PROSHIKA, Bangladesh

Start Date: 00-00-2006

End Date: 00-00-2007

Program Area: Variety and Species

Commodity: Fruit

Objectives:

The present research study was investigated to develop an efficient protocol using tissue culture technique for the large scale propagation of this year-round fruiting jackfruit variety through examining the effects of growth regulators and Murasighe and Skoog (MS) medium (Murashige and Skoog, 1962).

Methodology:

This experiment was carried out at the Plant Tissue Culture Laboratory of PROSHIKA, Bangladesh during 2006 to 2007. Explant Collection and Sterilization. 50-70 explants were collected for each experiment from 10-15 day-old newly sprouted healthy shoots from 10-12 years-old fruit-bearing trees growing at the Bangladesh Agricultural Research Institute (BARI). The actual number of explants used for any particular experiment is detailed in the accompanying tables. After removal of expanded leaves, the shoot apices and nodes were washed thoroughly under running tap water for 30 minutes and then treated with Tween 80 plus Savlon for 10 minutes and again rinsed with distilled water. The explants were surface disinfected with 0.1% HgCl2 (for 5 to 15 minutes) followed by seven times rinsing with sterile distilled water in a laminar airflow cabinet. The explants were finally trimmed, prepared and cultured on to the medium. Shoot Proliferation Stage. Explants were implanted vertically on MS medium supplemented with different (0.2, .05, 0.8, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5 mg/l) concentrations of 6-benzyl amino purine (BAP). All media were supplemented with 3% sucrose and 0.8% Difco Bacto-agar. The pH was adjusted to 5.8 prior to autoclaving the media at 1 kg pressure per cm2 and 121^oC for 20 minutes. All culture vessels were incubated in a growth room at 26 ± 2^o C with 16 hrs light provided by Philip white fluorescent tube lights with a photon flux density of 300 lux. Multiple Shooting. In a second experiment, jackfruit explants were cultured on MS medium fortified with different concentrations and combinations of BAP (3.0, 3.5, 4.0 mg/l) and Kinetin (Kn) from 0.2 to 3.0 mg/l to observe the synergistic effect on multiple shoot formation. Root Induction. For Root induction, micro-shoots were cultured on ½ MS medium enriched with different concentrations (0.2 to 2.0 mg/l) of Indole-3-butyric acid (IBA). Acclimatization. In vitro derived plantlets were gradually hardened through gradual exposure to optimum relative humidity and sunlight for seven days and then transferred to the net house. Plantlets were taken out from the culture vessels/tubes, washed carefully under running tap water to remove any traces of agar. Each plantlet was transplanted to a small poly-bag containing different composition and combination of soil media to select the best formulation. The plantlets were immediately covered with a perforated polythene bag to prevent desiccation. Open Field Observation. Acclimatized plants were observed for proper establishment in soil for fifteen days and field observation was done from January`07 to February`07 and data were recorded in 1-week intervals. Data Assemblage. Weekly growth observations were made up to four weeks after establishment and experimental data were recorded. The parameters were as: i. Shoot length in cm and number of leaves/shoot ii. An average number of multiple shoot/explant iii. The average length of multiple shoots in cm iv. An average number of root/shoot v. Average root length in cm. Recorded data were analyzed as mean ± SE according to Mian and Miyan (1984).

Source of Information: Department of Biotechnology & Genetic Engineering University of Development Alternative

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

With this experiment, a protocol for the micropropagation of a unique variety of A. heterophyllus, which fruits throughout the year has been established from shoot tip and nodal explants. It may be noted that all previous studies were conducted with varieties of this species, which fruits for only two to three months during January till December of any given year. To our knowledge, this is the first successful description of micropropagation of this unique variety. Successful micropropagation of this variety can lead to widespread cultivation and for obtaining this fruit throughout the year, which in turn can lead to a cheap source of nutritious food available to the general population and specifically to the nutritionally impoverished population of Bangladesh and other countries where this variety can be cultivated.

Knowledge Management: Report/Proceedings