Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Growth Performance of Spirulina Platensis in Three Different Concentrations of Banana Leaf Ash with Added Jackjfruit Seed Powder and Urea

M.A. Toyub
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

M.M. Rahman'
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

M.I. Miah
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh

M.A.B. Habib
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh 2202

Abstract/ Executive Summary:

The growth performance of Spirulina platensis was studied in three different concentrations of banana leaf ash (BLA) added with 0.4 g/I jackfruit seed powder (JSP) and 0.2 g/I urea in the laboratory. S. platensis was cultured in three different concentrations viz. 4.8, 7.2 and 9.6 g/1 of BLA and Kosaric medium (KM) as control with three replications for a period of three months. Each trial was done for a period of 12 days in the laboratory. The initial cell weight of S. platensis was 4 mg/1 which attained a maximum cell weight of 545 mg/1 in Kosaric medium followed by 523, 485, and 307 mg/1 in 7.2, 4.8, and 9.6 .mg/1 of BLA media with added 0.4 g/I JSP and 0.2 g/I urea respectively, on the 8th day of culture. Similar trend was observed in case of chlorophyll a content where the highest amount was 8.12 mg/I in KM and among different concentrations of BLA highest amount was 7.53 mg/I in 7.2 g/I BLA with added 0.4 g/I JSP and 0.2 g/I urea. The percentage of crude protein, crude lipid, moisture, ash and NFE contents of JSP were recorded 12, 1.85, 10, 3.62, and 72.53%, respectively. The percentage of total nitrogen, phosphorus, sulpher, sodium and calcium of BLA were recorded 0.18, 0.09, 2.35, 0.10, 0.07 and 10.81%, respectively. The result showed that the growth rate of S. platensis was significantly (p<0.05) higher in 7.2 g/1 BLA medium with 0.4 g/I JSP and 0.2 g/I urea than other concentrations of BLA. It is suggested that BLA algal culture medium is as effective as commercially produced culture medium making it feasible to culture Spirulina.

Keywords: Spirulina, Culture media, Cell weight, Chlorophyll

Location: In the laboratory of the Department of Aquaculture, BAU, Mymensingh

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Fertilizer and manures, Ash

Objectives:

An attempt was made to find out inexpensive organic media for Spirulina culture. The main objective of the experiment is to develop an inexpensive culture medium for large-scale production of Spirulina platensis

Methodology:

Preparation and analysis of Jackfruit seed powder (JSP) and banana leaf ash (BLA) Jackfruit seed powder and banana leaf ash was used as an organic ingredient. The collected seed was dried in an oven at 50°C for overnight. For complete drying of jackfruit seed, the sample was sun-dried for another seven days. The sample was made powder and sieved through a sieve of 500-micrometer mesh size for getting very fine particle of jack-fruit seed powder. Proximate composition (moisture, crude protein, crude lipid and ash) of jackfruit seed sample was analyzed in the nutrition laboratory following the standard methods (Horwitz, 1984). Collected banana leaf was chopped with knife and dried in the sunshine for seven days and dried banana leaf was burnt to make ash. Minerals (total nitrogen, P, S, K, Na and Ca) of the sample was analyzed in the soil science laboratory following standard methods.

Preparation of media: Three different media of banana leaf ash (BLA) with added jackfruit seed powder (JSP) and urea, and Kosaric medium (KM) (Clesceri et al. 1989) were prepared for Spirulina platensis culture. For the preparation of the three different BLA media, 4.8, 7.2 and 9.6 g of BLA were weighed in 1.0 litre conical flask. Then 0.4 g JSP and 0.2 g urea were added in 'every flask and distilled water was added to make the volume up to 1.0 litre mark. Then the media were mixed well and autoclaved at 120°C for 15 minutes with moist heat. After autoclaving, flasks were kept for 24 hrs. for cooling before culture of microalgae. Mixing, sterilization and cooling of the prepared KM was done following the same procedures.

Culture of Spirulina platensis The experiment was conducted in the laboratory of the Department of Aquaculture, BAU, Mymensingh. Four treatments, three from three various concentrations of banana leaf ash containing 0.4 g/I JSP and 0.2 g/I urea and one from KM as control were used with three replications to culture S. platensis in 1.0 litre conical flask. S. platensis were then inoculated into the flasks to produce a culture containing 10% S. platensis suspension (OD at 620 nm = 0.20) (Habib, 1998). All the flasks were kept under fluorescent lights (light: dark = 12: 12) in the laboratory. The culture flasks were continuously aerated. Eight sub sample were taken at every alternate day from each flasks to observe cell weight and chlorophyll-a of S. platensis and physico-chemical properties of culture media. Temperature, light .intensity, dissolved oxygen and pH were determined by respective meters. Nitrite-nitrogen, nitrate-nitrogen and Phosphate-phosphorus were determined by Hach kit (DREU2000). Spirulina cells were weighed using GFC filter paper and chlorophyll a was determined by following Clesceri et al. (1989).

Statistical analysis Differences among the treatments of S. platensis in different media were analyzed by one way ANOVA (Gomez and Gomez, 1976).

Source of Information: J. Bangladesh Agril. Univ. 3(2): 303-308, 2005 ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

So, it is concluded that the growth of S. platensis was maximum in 7.2 g/I BLA medium added with 0.4 g/1 JSP and 0.2 g/1 urea than other media. Thus, this concentration may be recommended for cu!ture of S. platensis though the control KM gave the highest (p<0.05) growth.

Knowledge Management: Journal