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Assessment of Stress-tolerance Attributes in Wheat Using Gene-specific Molecular Markers (2014-2015)

Rona Mahmud
Biotechnology Division, BARI, Joydebpur, Gazipur

M.A.Y. Akhond
Biotechnology Division, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

The experiment was conducted for heat shock protein based SNP marker for terminal heat stress in twenty two local and two Australian wheat varieties. Attempts were made to identify single nucleotide polymorphism (SNP) and to differentiate the heat tolerant and heat susceptible genotypes of wheat using heat shock protein (HSP16.9) as the target gene. DNA fragment covering a partial sequence of Triticum aestivum L. HSP 16.9, were amplified and allele specific primers based on the SNP were used to screen the heat tolerant and susceptible genotypes. Results obtained from this HSP derived SNP marker associated with terminal heat stress in wheat can be used by the breeders for improving tolerance to high temperatures in wheat breeding programmes.

Keywords: PCR analysis, Mini Kit protocol , Electrophoresis

Location:

Start Date: 00-00-2014

End Date: 00-00-2015

Program Area: Variety and Species

Commodity: Wheat

Objectives:

Attempts were made in this experiment to identify single nucleotide polymorphism (SNP) and to differentiate the heat-tolerant and heat susceptible genotypes of wheat using heat shock protein (HSP16.9) as the target gene.

Methodology:

DNA was extracted from leaf tissue of 24 wheat varieties, using Genomic DNA Mini Kit protocol and PCR analysis was carried out using specific primers following standard protocols. The amplified products were separated by electrophoresis on 1% (w/v) agarose gel and stained with ethidium bromide and analyzed under UV light.

Source of Information: Annual Research Report 2014-2015, Biotechnology Division, BARI, Joydebpur, Gazipur

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

PCR analysis was carried out to identify a single product of 197 bp from selected 24 wheat varieties for differentiating the heat-tolerant and heat susceptible genotypes by using specially designed primers. Amplification of the specific band indicated the heat susceptible genotypes and the samples which did not amplify the band indicated the heat-tolerant genotypes. Further research is necessary for confirmation of the initial findings.

Knowledge Management: Report/Proceedings