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Validation Trial of Tissue Cultured Plantlets of BARI Malta-1 (2014-2015)

M.M.H. Molla
Biotechnology Division, BARI, Joydebpur, Gazipur

A. Y. Akhond
Biotechnology Division, BARI, Joydebpur, Gazipur

D. Khanam
Biotechnology Division, BARI, Joydebpur, Gazipur

M. M. Khatun
Biotechnology Division, BARI, Joydebpur, Gazipur

A.S.M.H. Rashid
Biotechnology Division, BARI, Joydebpur, Gazipur

Z.A. Firoz
Biotechnology Division, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

Tissue cultured and grafted saplings of BARI malta-1 have been planted to see the field performance at ARS, Ramgarh and ARS, Raikhali. Both tissue cultured and grafted plants of BARI malta-1 produced fruits in the third year. The highest no. of fruits/plants (35.7) was found in tissue cultured plants followed by grafted one (6.5) in ARS, Ramgarh. Maximum plant height (219 cm), no. of brunches (8.50) and base girth (17.20 cm) was found in tissue cultured plants. Grafted plants produced 192 cm plant height along with 8.75 cm base girth and 6.4 branches. Vigorous growth and development has been found in tissue-cultured plants compare to grafted ones during third year ( Tissue culture: N-S = 162.75 cm, E-W = 149 cm; grafted: N-S = 143.25 cm, E-W = 141 cm). In the case of ARS, Raikhali, maximum plant height (243 cm), no. of brunches (10.0) and base girth (22.42 cm) were found in tissue cultured plants in the third year. The highest plant canopy N-S= 292 and E-W= 292.5 cm was found in grafted plants where tissue culture plants have N-S= 298.17 cm and E-W= 297.14 cm (Table 2). However, vigorous growth and development were found in tissue cultured plants compare to grafted ones. Both tissues cultured and grafted plants produced flowers and fruits after three years of planting.

Keywords: Plantlets, Malta, Tissue Culture

Location:

Start Date: 00-00-2014

End Date: 00-00-2015

Program Area: Variety and Species

Commodity: Orange

Objectives:

The investigation was undertaken to see the field performance of tissue cultured plantlets of BARI Malta-1 compared to grafted one.

Methodology:

The regenerated rooted plantlets with 5-6 leaf stage were transferred from the culture room and kept at room temperature (30⁰C) for 5-7 days. The plantlets were then removed from the culture tubes and all the adhering media were carefully washed out so that, the root damage was the least. Washed plantlets were planted into small polyethylene bags containing sterile soil, sand and decomposed cow dung at the ratio of 1: 1: 1. Subsequently, the healthy plants were transplanted in a 9 x 12 inch earthen pots containing soil mixture at the nursery for three months. Well-established saplings were transplanted along with grafted one at Hill Agricultural Research Station, Raikhali, Rangamati Hill Districts and Hill Agricultural Research Station, Ramgarh, Khagrachari Hill Districts in May 2011. Saplings were transplanted at a single-line design following plant to plant distance of 4.0 m. A single plant was considered as one replication. Data on Plant height, base girth and number of branches/plant were recorded.

Source of Information: Annual Research Report 2014-2015, Biotechnology Division, BARI, Joydebpur, Gazipur

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Cent percent (100%) transplanted saplings were survived at field conditions. Data on growth and development have been presented. Maximum plant height (219 cm), no. of brunches (8.50) and base girth (17.20 cm) was found in tissue cultured plants. Grafted plants produced 192 cm plant height along with 8.75 cm base girth and 6.4 branches. Plant canopy was also found higher in tissue cultured plants ( N-S = 162.75 cm, E-W = 149 cm) compare to grafted ones (N-S = 143.25 cm, E-W = 141 cm. However, vigorous growth and development have been found in tissue-cultured plants compare to grafted ones during the third year. Both tissue culture and grafted plants of BARI malta 1 produced fruits in the third year. The highest no. of fruits/plant (35.7) was found in tissue culture plant followed by in grafted one (6.5). In the case of ARS, Raikhali, maximum plant height (243 cm), no. of brunches (10.0) and base girth (22.42 cm) were found in tissue cultured plants in the third year. The highest plant canopy N-S= 292 and E-W= 292.5 cm was found in grafted plants where tissue culture plants have N-S= 298.17 cm and E-W= 297.14 cm. However, vigorous growth and development were found in tissue cultured plants compare to grafted ones. Both tissue cultured and grafted plants produced flowers and fruits after three years of planting. But due to unavoidable circumstances, all fruits were dropped.

Knowledge Management: Report/Proceedings