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Regulatory Trial with Transgenic Potato Under Cft 2014-15 (2014-2015)

T.K Dey
Biotechnology Division, BARI, Joydebpur, Gazipur

M. Dhar
Biotechnology Division, BARI, Joydebpur, Gazipur

J.H Prodhan
Biotechnology Division, BARI, Joydebpur, Gazipur

S. hossain
Biotechnology Division, BARI, Joydebpur, Gazipur

M. M. Khatun
Biotechnology Division, BARI, Joydebpur, Gazipur

B. Anawer
Biotechnology Division, BARI, Joydebpur, Gazipur

A. Kawsar
Biotechnology Division, BARI, Joydebpur, Gazipur

H. Raihan
Biotechnology Division, BARI, Joydebpur, Gazipur

S.C Halder
Biotechnology Division, BARI, Joydebpur, Gazipur

D. Khanam

Abstract/ Executive Summary:

Regulatory trial under Confined field (CFT) was conducted at six locations (Gazipur, Bogra, Jessore, Rangpur, Comilla and Chittagong of Bangladesh Agricultural Research Institute) with six transgenic RB hybrid clones of potato. The clones were: D-951 (2), D-951 (3), D-951 (12), D-951 (13), D-951 (137) and D-951 (304). Non-transgenic BARI Alu-7 (Diamant) ) was used as a check. Seed tubers were planted from December 2 to 8, 2014. The experiment plot was laid out in RCB design having three replications. As this year trial was a regulatory perspective, so nontransgenic Diamant (check) was sprayed alternating with fungicides Secure (0.1%) and Dithane M-45 (0.2%) from initiation of late blight to keep it free from the disease. Incidence of late blight appeared with different degree became well pronounced in five locations (Gazipur, Bogra, Jessore, Comilla, Rangpur, and Comilla) out of six locations. There was no late blight infection at Hathazari,Chittagong. Initiation of disease symptom detected at 35 to 70 days of plant age in border crop Diamant where no fungicide was applied. In each location nontransgenic border crop Diamant was damaged 100% while hybrid clones performed well to late blight having distinguishable lower AUDPC value. AUDPC value was less than 100 in all five locations except Bogra. Many insect pests and beneficial insects (aphid, ladybird beetle, cutworm, whitefly etc.) visited in all RB hybrid clones along with the check variety, indicating RB hybrid clones had no harmful effect on insects. PLRV, PVY and PVX were present sporadically, this might be caused by secondary infection. Bacterial wilt, soft rot, stem canker, dry rot was present irregularly in different locations over Bangladesh. Common scab was present in all the hybrid clones as well as check, Diamant. Morphological and tuber characteristics were almost similar in both transgenic clones and nontransgenic Diamant. Regarding yield, it varied to some extent over locations in both transgenic clones and nontransgenic Diamant.

Keywords: On-Farm, Transgenic, Potato

Location:

Start Date: 00-00-2014

End Date: 00-00-2015

Program Area: Variety and Species

Commodity: Potato

Objectives:

The present study was undertaken with the purpose to establish substantial equivalence between the transgenic and conventional lines which means that the transgenic lines are not different from their counterpart due to the insertion of new genes.

Methodology:

The experiment was conducted at six locations namely, On-Farm Research Division (OFRD), Bogra; Regional Agricultural Research Station (RARS), Burirhat, Rangpur; Research Field of Biotechnology Division, BARI, Gazipur; ARS, Comilla; RARS, Jessore and Hathazari, Chittagong during rabi season of 2014-15. Six transgenic RB hybrid clones of potato viz. D-951 (2), D-951 (3), D-951 (12), D-951 (13), D-951 (137) and D-951 (304) along with the check, BARI Alu-7 (Diamant) were tested. Before sowing the seed, the whole experimental plot was confined by an iron net to protect crops from humans and animals. The experiment was laid out in RCB design with three replications, having plot size 3.0 m ´ 1.8m. Seeds were planted between December 2 to 8, 2014. The spacing of row to row and seed to seed was 60 cm and 30 cm, respectively. To fulfill the requirements of regulatory perspective, nontransgenic Diamant under trial was sprayed with fungicides ( alternating Secure (0.1%) with Dithane M 45(0.2%), starting just after initiation of blight symptom at all locations except Bogra. At Bogra the crop was already infected by late blight 10 days earlier before we received instruction of fungicide application in nontransgenic Diamant. Transgenic hybrids were sprayed with plain water. Incidence of late blight was recorded following a modified blight rating scale at 2 and 3 days intervals from the onset of disease symptoms and continued up to disease standstill at a point. Ten plants were selected randomly from each plot to record the number of insect pests and beneficial insects. One compound leaf from each selected plant was randomly used for counting aphid, ladybird beetle and lacewing. A number of diseased plant/plot was recorded except late blight. Area Under Disease Progress Curve (AUDPC) value was calculated following standard formula. Harvesting was done on March 3 (Bogra), March 10 (Rangpur), March 15 (Comilla), March 8 (Chittagong), March 12 (Jessore), and March 10 (Gazipur), 2015. All the haulms were gathered in dump-site at the corner of the experimental plot and then set fire. At harvest, 5 plants were selected randomly from each plot to record the number and weight of tuber/plant. A number of disease tubers/plots was also recorded. The yield (t/ha) and dry matter (%) were recorded. Data were analyzed statistically and the treatment means were separated by Duncan’s Multiple Range Test (DMRT) or Least Significance Difference (LSD) test.

Source of Information: Annual Research Report 2014-2015, Biotechnology Division, BARI, Joydebpur, Gazipur

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Significant variation was found among the tested varieties/lines in all characters except emergence at 50 days after planting (DAP). Plant vigor (at 45 DAP) in all hybrid clones and Diamant was very good except D 951(304) with moderate growth. Foliage coverage (at 50 DAP) highest in D 951(137) which was statistically similar to D 951(12), D 951(13), D 951(2), D 951(3) but differed significantly with D 951(304) and Diamant. Only 50% foliage coverage was observed in Diamant. The highest plant height was observed in D 951(3) (54.90 cm) which was statistically at par with D 951(2) but differed significantly from other treatments. Stem diameter was highest in D 951(2) (1.31 cm) and it showed statistically insignificant with D 951 (3), D 951 (12) and D 951 (13). The number of stem/plant was maximum in Diamant (4.8) which was statistically similar to D 951(13) and D 951(137). The maximum number of tuber/plant was observed in Diamant (11.5/plant) which was statistically similar to D 951(13) (9.83) but differed significantly from the rest lines. D 951(137) produced the highest weight of tuber/plant (0.62 kg/plant) which was statistically at par with D 951(13) and D 951 (12) whereas the lowest recorded in Diamant. The maximum and minimum percentage of dry matter were obtained from D 951(304) (17.86%) and Diamant (14.86%), respectively. A significantly higher yield of 31.97 t/ha was recorded in D 951(137) and it showed statistically insignificant with D 951(13) and D 951(12) but differed significantly from the rest hybrid clones. The lowest yield was obtained from D 951(304) (14.19 t/ha) which was statistically similar to Diamant (17.34 t/ha) and differed from all hybrid clones. The minimum AUDPC value of 116.27 was computed in D 951(304) and D 951(137) (119.85) whereas the maximum in D 951(2) (669.89) and D 951(3) (550.35). In Diamant AUDPC value was 203.29.

Late blight infection was first noticed at 35 DAS in Diamant. In border crop Diamant, about 25% foliage was damaged within 48 DAS, 50% at 55 DAS, 75% at 61 DAS, 95% at 64 DAS and 100% damaged at 83 DAS in border crop Diamant where AUDPC value was 1325.65. In LBR clones, disease infection was first observed at 38 DAS but the progress of the disease was very slow. Results revealed that hybrid clones performed better under field conditions. D 951 (304) and Diamant produced a lower yield than other hybrid clones. Severe infection (32%) of virus disease was observed in D 951 (304) which produced the lowest no of tuber and offered the lowest yield. Diamant produced a higher number of tuber per plant but the varieties infected by late blight at an early stage, as a result, the variety produced a very small size of tuber and offered minimal yield. There were no remarkable variations in morphological traits (stem shape, stem diameter and stem colour) among transgenic hybrid clones and their counterpart, Diamant (Table 2). Many insect pests (aphid, housefly, Monolepta beetle, whitefly, flea beetle, red pumpkin beetle etc.) and beneficial insects (ladybird beetle, syrphid fly etc.) and many diseases were observed in all Rb hybrid clones along with the Diamant, it means that Rb hybrid clones had no harmful effect on insects and diseases as well as invitation of any new pests. Regarding tuber characteristic traits, almost similar observations became evident in both transgenic hybrid clones and nontransgenic Diamant

Knowledge Management: Report/Proceedings