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In Vitro Regeneration of Mirabilis Jalapa L.

M. S. Rahman
Industrial Botany Research Division, BCSIR laboratories Chittagong, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka

N. C. Nandi
Medicinal and Aromatic Plant Research Division BCSIR Laboratories Chittagong, Bangladesh Council of Scientific and Industrial Research (BCSIR)

B. Goswami
Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka-1205

Abstract/ Executive Summary:

In vitro regeneration of Mirabilis jalapa L., an important medicinal plant, has been successfully established from nodal explants. Maximum numbers of shoots (8.1) were obtained using Murashige and Skoog (MS) medium containing BAP (3.0 mg/l). Next to the highest response by nodal explants of M. jalapa was shown on MS medium supplemented with 2.0 mg/l BAP +1.0 mg/l IAA + 1.0 mg/l Kn. In it, the mean value of shoot number was 7.3. The highest frequency of root induction (80%) were obtained on MS medium supplemented with IAA (0.5 mg/l) and 40 ml/l coconut water. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully planted in the field.

Keywords: Mirabilis; In vitro regeneration; Nodal explants; Multiple shoots

Location: Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Medicinal Plants

Objectives:

To develop an efficient protocol for in vitro regeneration of M. jalapa using nodal explants.

Methodology:

The present experiment was conducted in Bangladesh Council of Scientific and Industrial Research (BCSIR), Chattogram, Bangladesh. Nodal explants with axillary buds of M. jalapa were collected from BCSIR research field. Nodal segments (1-2 cm long) without leaves were surface sterilized initially with mild detergent and washed with tap water for 30 min. After that, explants were surface sterilized with 70% (v/v) ethanol for 1 min, followed by 0.1% HgCl₂ by gentle shaking for 8-10 min, and rinsed four times with autoclaved distilled water. Isolated explants were cultured on MS media (Murashige and Skoog, 1962) containing BAP, NAA, IAA and Kn singly or in combinations for in vitro regeneration of shoots. MS media supplemented with coconut water in combination with hormones were also used for shoot regeneration. In vitro regenerated shoots were sub-cultured regularly to fresh medium at an interval of 12 - 15 d for further multiplication. Elongated shoots were separated and cultured on rooting medium for root formation. About 2-3 cm long shoots were separated and cultured on rooting medium containing full strengths of MS without hormonal supplement or with different concentrations of IAA and IBA. All in vitro grown cultures were maintained under illumination on a 12 h photoperiod at 25 ± 2^ºC. The plantlets with sufficient root system were then transplanted to small plastic pots containing sterilized soil for their establishment. Twenty plantlets were transplanted into small plastic pot for hardening. After 30 days, the acclimatized plants were transferred to larger pots containing of sterile peat and sand in a ratio of 1:1 and the acclimatized plantlets were watered and fertilized as needed.

Source of Information: Bangladesh J. Sci. Ind. Res.56(1), 25-28, 2021

Article Link (Online Journal): DOI: https://doi.org/10.3329/bjsir.v56i1.52692

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It can be concluded that the present in vitro regeneration protocol of M. jalapa is efficient and reproducible. This regeneration protocol reported in the present study can be used for the large-scale production of this medicinal plant within a short time and to yield important secondary metabolites for pharmaceutical industries with a range of further biotechnological applications.

Knowledge Management: Journal