Md. Mominur Rahman
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Abu Rayhan
Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M. N. Hossen
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M. F. Siddique
Senior Scientific Officer
Bangladesh Fisheries Research Institute, Mymensingh-2201, Bangladesh
Md. Ali Reza Faruk
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Mehedi Islam
Product Executive Aggravate Division, Square Pharmaceuticals Ltd, Bangladesh
Aqua drugs, Chemicals, Fish, Pond
Muktagacha, Fulbaria and Fulpur of Mymensing district, Bangladesh
Animal Health and Management
Three upazilas like Muktagacha, Fulbaria and Fulpur of Mymensing district were selected for the present study.
Questionnaire survey: Fish farmers, hatchery and nursery owner, farm workers and chemical retailers were questioned individually. A set of preliminary questionnaire based on the objectives of the study was prepared. Major topic of questionnaire were the name of chemical, active ingredients, purpose of use, method of application and dose, duration, source, effects on environment, price, impact on health and productivity etc. In addition data on farming practices, general farm management, health and disease problems, seasonality, mortality etc were also gathered. For the interview, simple random sampling methods were followed.
Drug samples: Drug used for management and production of fish by the farmers were collected from local market of the study areas. The drugs of different companies were collected.
Fish health and disease investigation In addition with the field observation, fish health and disease condition were verified through clinical and histopathological analysis. Fish were examined clinically through observing gross signs, abnormalities, lesions and erosions and external parasites.
Histological observation For histopathological study, monthly sample was collected from fishes of selected farmer’s pond and various organs such as skin, muscle, gill, liver and kidney by a sharp scalpel and forceps. Skin and muscle were collected from the place between anterior part of dorsal fin and lateral line and gills samples were collected by removing operculum. For liver and kidney, fishes were dissected and then portions of liver and kidney were collected. All collected samples were fixed in 10% natural buffered formalin and proceeded for preparing histological slide. The slides were then examined under a compound microscope. Photomicrographs of the stained sections were done by using a photomicroscope. Comparisons of structure and pathology of organs were made among treatments.
International Journal of Applied Research 3(2) 50-55
Journal