Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Development of an Efficient Regeneration System of Banana (2016--2017)

M. M. Khatun
Biotechnology Division, BARI, Joydebpur, Gazipur

S. Islam
Biotechnology Division, BARI, Joydebpur, Gazipur

M. R. Kabir
Biotechnology Division, BARI, Joydebpur, Gazipur

D. Khanam
Biotechnology Division, BARI, Joydebpur, Gazipur

M. A. Y. Akhond
Biotechnology Division, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

The present study was undertaken with a view to establishing a reliable regeneration protocol of banana (Musa spp.). The bottom portion and whole male flower buds were used as explants for callus induction. Explants were cultured on MS medium supplemented with different concentrations of 2, 4-D singly and in combination with different concentrations of IAA and NAA. In the Bottom portion of male flower buds, among the four different treatments, the highest percentage of explant produced callus in T₂ treatment (56%) compared to other treatments. In whole male flower buds, the highest percentage of explants produced callus in T₁ and T₂ treatment (100%) compared to other treatments.

Keywords: Laminar airflow, Banana, Explant

Location:

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Variety and Species

Commodity: Banana

Objectives:

The present study has been undertaken to develop an efficient regeneration protocol for banana which will be used for the development of banana transgenic with desirable traits.

Methodology:

Male flower buds of banana variety Maher Sagar were used as source material. The fresh male flower buds were collected at the time of complete bunch emergence. Outer enveloping bracts were removed and surfaces were cleaned with absolute alcohol. Under the laminar airflow cabinet, the remaining outer enveloping bracts were removed and the inner part (2-3 cm in length) containing male flower primordia was isolated. Individual male flower primordia were excised and cultured on MS medium supplemented with different concentrations of 2, 4-D and in combination with IAA and NAA for initiation of callus as well as regeneration of shoot. The p^H of all media was adjusted to 5.8 before autoclaving. The media were autoclaved at 121⁰C and 1.06 kg cm^-2 pressure for 20 minutes. All cultures were incubated at 25±2⁰C in complete darkness and maintained on the same medium without subculture until callus formed.

Source of Information: Annual Research Report 2016-2017, Biotechnology Division, BARI, Joydebpur, Gazipur

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The bottom portion of individual male flower buds was cultured on MS medium supplemented with four different concentrations of 2, 4-D showed enlargement of the explants and the development of whitish callus after one month of inoculation. Among the treatments, maximum callus was found in T₂ treatment (56%) followed by T₄ (50%). The lowest calli were noticed in treatment T₁ (31%). combination with IAA and NAA to find out the suitable culture medium for callus induction. Male flower buds cultured on MS medium supplemented with 2, 4-D and four different concentrations of IAA and NAA showed enlargement of the floral primordia and the development of whitish callus after one month of inoculation. Among the treatments, maximum callus was found in T₁ and T₂ treatment (100%). The lowest calli were noticed in treatment T₃ (44%). The calli were then separated and transferred to a fresh MS medium containing different concentrations of BAP, IAA and casein hydrolysate in order to find suitable media composition for regeneration of shoots. In this medium, all calli turn greenish within two weeks of culture. But no shoots were produced.

Knowledge Management: Report/Proceedings