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Effect of Heat Stress on Protein Expression in Potential Bangladeshi Tomato Varieties (2016--2017)

S. Islam
Biotechnology Division, BARI, Joydebpur, Gazipur

M.R. Kabir
Biotechnology Division, BARI, Joydebpur, Gazipur

D. Khanam
Biotechnology Division, BARI, Joydebpur, Gazipur

M.A.Y. Akhond
Biotechnology Division, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

The experiment was conducted with a view to determine the effect of heat stress on protein expression in tomato leaves and other plant parts and to find out the cultivar differences at protein expression level under heat stress conditions. The experiment was conducted based on the expression of heat shock protein in BARI released promising summer variety and high yielding tomato varieties. Four BARI released promising tomato varieties and their four parental lines were selected for this experiment. Attempts were made to identify heat shock protein and to differentiate the heat-tolerant tomato varieties in the context of overly expressed heat shock protein. Total plant proteins including large and small heat shock proteins were extracted and SDS-PAGE was done to get an idea about the extracted proteins. Results obtained from this experiment can be used for further experiments in this field.

Keywords: Heat tolerant, Heat shock protein, Tomato

Location:

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Variety and Species

Commodity: Tomato

Objectives:

Attempts were made to identify heat shock protein and to differentiate the heat-tolerant tomato varieties in the context of overexpression of heat shock protein under heat stress conditions.

Methodology:

In this experiment, BARI Hybrid tomato-4 and its parental lines, BARI Hybrid tomato-8 and its parental lines, BARI tomato- 14, BARI tomato-15 was used as germplasm. Seedlings of all the tomato varieties were grown in the same environmental condition and protein was extracted from leaf tissue of those 8 genotypes, using TCA/Acetone Protein Precipitation Protocol(Damervalet al., 1986). Extracted protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 13% polyacrylamide gel and stained with Coomassie Brilliant Blue staining solution and analyzed under White light.

Source of Information: Annual Research Report 2016 2017, Biotechnology Division, BARI, Joydebpur, Gazipur

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Under this experiment, standardization of Protein extraction and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol has been done. Protein extraction and SDS-PAGE were done to identify the difference among the BARI released tomato varieties at protein expression level for differentiating heat tolerant and heat susceptible varieties. There is no significant difference has been found among the tomato varieties at the protein expression level. Further research is necessary for finding out the difference among the tomato varieties, especially at the tomato heat shock protein (Hsps) expression level.

Knowledge Management: Report/Proceedings