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Standardization of Micropropagation Protocol for BARI Strawberry Varieties and Their Large Scale Multiplication (2017--2018)

M. M. Khatun
Biotechnology Division, BARI, Joydebpur, Gazipur

A. Saha
Biotechnology Division, BARI, Joydebpur, Gazipur

S. Yesmin
Biotechnology Division, BARI, Joydebpur, Gazipur

B. Sarker
Biotechnology Division, BARI, Joydebpur, Gazipur

D. Khanam
Biotechnology Division, BARI, Joydebpur, Gazipur

Abstract/ Executive Summary:

Shoot tips of BARI Strawberry-2 and BARI Strawberry-3 were cultured on MS medium supplemented with different concentrations and combinations of BAP, Kn and GA₃. Four different treatments T₁, T₂, T₃, & T₄, were tested for shoot multiplication and elongation. In case of BARI Strawberry-2, for shoot formation 1.0 mg/l BAP + 0.5 mg/l Kn +1.5 mg/l GA₃ combination was found most suitable. Whereas in BARI Strawberry 3, 0.5 mg/l BAP + 0.5 mg/l Kn +1.5 mg/l GA₃treatment was found most suitable for shoot formation. Half MS medium without hormone was found enough for root induction.

Keywords: Laminar airflow, Strawberry, Propagation

Location:

Start Date: 00-00-2017

End Date: 00-00-2018

Program Area: Variety and Species

Commodity: Strawberry

Objectives:

The present experiment is important to develop a suitable protocol for the mass propagation of BARI strawberry varieties.

Methodology:

BARI Strawberry-2 and BARI Strawberry-3 were collected from the fruits field of HRC, BARI and used as plant materials. Small shoot tip came out from runner were carefully removed by scalpel from Strawberry plants showing no virus symptoms. The explants were prepared by removing the outer layers of tissue from the shoot tips by the scalpel. The light greenish 2-3 cm shoot tips were surface sterilized with Ridomil Gold MZ 68WG for 5 min. to remove the fungal contamination. Then shoot tips were sterilized with Jet and Trix under running tap water for 5-6 min followed by 3-4 times washing with distilled water. After then shoot tips were sterilized with Clorox solution (60 ml Clorox: 40 ml distilled water) with 2 drops of Tween-20 for 3 min. followed by 3-4 times washing with sterile distilled water inside the laminar airflow cabinet. The dead tissue of sterilized explants was cut off to leave 1-2 cm portion containing an intact apex. The prepared explants were placed in MS media (Murashige and Skoog, 1962) with four concentrations (T₁, T₂, T₃, and T₄) with combinations of BAP, Kn and GA₃. All cultures were incubated at 25 ± 1⁰C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes. The pH of the medium was adjusted to 5.8.

Source of Information: Annual Research Report 2017-2018, Biotechnology Division, BARI, Joydebpur, Gazipur

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Shoot tip explants of BARI Strawberry-2 and BARI Strawberry-3 were cultured on MS medium supplemented with four different concentrations and combinations of BAP, Kn and GA₃. Shoot proliferation: Shoot initiation started within 6-7 days after inoculation. In BARI Strawberry-2, the highest plant height (2.54 cm) was observed in T₂ treatment and the lowest (1.86 cm) was obtained from T₄ treatment. The average node number was highest in T₂ treatment (4.6) followed by T₃ (4.4). On the other hand, the lowest node number (3.0) was obtained from the T₄ treatment. In leaf number, the maximum leaf (6.4) was found in T₂ treatment and the lowest (5.0) was obtained from T₄ treatment. In BARI Strawberry-3, the highest plant height (2.68 cm) was observed in T₁ treatment and the lowest (2.40 cm) was obtained from T₄ treatment. The average node number was highest in T₁ treatment (3.2) followed by T₃(3.0). On the other hand, the lowest node number (2.6) was obtained from the T₄ treatment. In leaf number, the maximum leaf (4.4) was found in T₁ treatment followed by T₃(4.0) and the lowest (3.4) was obtained from T₂ treatment. In vitro grown shoots were cultured in ½MS without hormone for rooting. The result indicated that in strawberry rooting no growth hormone needed. In ½MS medium, roots produced spontaneously.

BARI Strawberry-2 performed better in T₂ treatment while BARI Strawberry-3 performed better in T₁ treatment. Both are at the multiple shoot formation stage. In BARI Strawberry-3, some shoots produced root. The experiment will be continued for more observation in the coming year.

Knowledge Management: Report/Proceedings