Md. Harunar Rashid*
Vererinary Surgeon, Gupalpur, Tangail, Bangladesh
Ashish Kumar Das
International Centre for Diarrhoeal Disease Research, Mohakhali, Dhaka, Bangladesh
M.A. Matin
Department of Dairy and Poultry Science, Patuakhali Science and Technology University, Babugonj, Barisal-8210, Bangladesh
Kanika Sammadar
Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Babugonj, Barisal-8210, Bangladesh
Nazim Ahmad
Vererinary Surgeon
Gupalpur, Tangail, Bangladesh
Liver fluke, GI nematode, Hematological, Biochemical profile, Bangladesh
Department of Physiology, BAU, Mymensingh
Animal Health and Management
Sheep, Nematodes
A total of 15 indigenous sheep were collected from the Department of Pharmacology, BAU, Mymensingh. The sheep were maintained in mixed flock mostly free grazing system. The sheep were of either sex and aged between 1 and 3 years and body weight ranged 10 kg to 15 kg. The sheep were divided into three equal groups as control (n=5), liver fluke infected (n=5) and gastrointestinal nematode infected (n=5).
Collection and examination of faecal sample: The faeces from the suspected animals were collected directly from the rectum and examined by direct smear and flotation sedimentation techniques. The fascioliasis and the gastrointestinal nematodiasis were diagnosed on the basis of morphological characteristics of parasitic eggs as described by Soulsby (1986) and Samad (1996). The animals which were found negative for fasciola and gastrointestinal parasitic infection on faecal examination served as controls group. Ear tag was used to identify the individual sheep.
Anthelmintic trials Fasciola infected sheep were treated with triclabendazole (Fasinex®- Novartis Bangladesh Ltd.) @ 12 mg/kg body weight orally. Gastrointestinal nematode sheep were treated with combination of tetramisole and oxyclozanide (Levanid®- The ACME Laboratories Ltd.) @ 7.5 mg/kg body weight orally.
Collection of blood Blood samples of each animal (infected and control) were collected aseptically from the jugular vein before administration of anthelmintics and on 30th days post-treatment, simultaneously in two separate tubes, one with double oxalate as anticoagulant and the other tube without adding any anticoagulant. The haematological studies were performed within two hours of blood collection.
Preparation of serum About 5-6 ml of blood was collected in the sterile glass test tubes. The blood containing tubes were placed in a slanting position at room temperature for 6 hours. The tubes were incubated over night in the refrigerator (4°C). The serum samples were separated and centrifuged to get rid of unwanted blood cells. Serum samples were stored at -20°c for biochemical analysis.
Hematological studies The hematological investigation of Total erythrocyte count (TEC), Hemoglobin (Hb), Estimation, Erythrocyte Sedimention Rate ( ESR ), Packed cell volume ( PCV ), Total leukocyte count (TLC) were performed within 2 hours of blood collection following the procedure described by Lamberg and Rothstein (1977). Biochemical studies The biochemical parameters of enzyme system like SGOT (Serum Glutamate Oxaloacetate Transaminaes) and SGPT (Serum Glutamate Pyruvate Transaminase) determinations were performed in collaboration with Safeway Diagnostic Complex, Charpara, Mymensingh. All the tests were performed colorimetrically using Humalyzer 2000 (Human type, Germany) following known techniques (Bergmeyer and Horder, 1980).
Statistical Analysis: A paired t-test was done for exploring the change on the haemato-biochemical parameters of the infected sheep after treating them by triclabendazole for F.gigantica and oxyclozanide with tetramisole for gastrointestinal nematode (Petrie and Watson, 1999). These parameters of the infected sheep were compared with that of control by two independent sample t-test.
2015 © International Journal of Applied Research 1 (1): 9-11
Journal