Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Xylo-oligosaccharides (xos) on Growth Performance, Blood Biochemistry and Total Viable Count in Ileum and Caecum of Broiler Chickens from Day 13-26

M. Akter
Department of Dairy and Poultry Science, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi-4225, Chattogram, Bangladesh

N. Akter
Department of Dairy and Poultry Science, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Khulshi-4225, Chattogram, Bangladesh

Abstract/ Executive Summary:

This study was undertaken to investigate the effect of xylo-oligosaccharides (XOS) on the growth performance, blood biochemistry and total bacterial count in the intestine of broiler chicken from 13 to 26 days of age. A total of 96 day-old Cobb 500 broiler chicks were distributed randomly into four treatment groups with four replicates (6 birds replicateG¹) and fed basal diet (control) from day 1-12. Test diets were formulated with four different levels of XOS (0, 2.5, 5.0 and 7.5 g kgG¹) and offered to the birds from day 13-26. On day 26, the data on growth performance, serum biochemical profile, total viable count in the ileum and cecum, visceral organ weight and carcass yields of the broiler chickens were assessed. There was no effect (p>0.05) of XOS on weight gain and feed intake. However, supplementation of 2.5 g XOS kgG¹ of diet significantly improved the feed conversion ratio (FCR) of the birds. This group of diet also increased (p<0.05) the serum concentration of T₃ and T₄ but reduced (p<0.05) the glucose level. Diets containing 2.5 and 5 g XOS kgG¹ increased the total viable count in both ileum and cecum. The dressing percentage and relative weight of the pancreas were significantly improved in birds consumed a diet containing 2.5 g XOS kgG¹. The abdominal fat content was low (p<0.05) in birds fed diets containing 2.5 and 5 g XOS kgG¹. Dietary XOS supplementation (2.5 and 5 g kgG¹ of diet) can improve the thyroid hormone activity and total viable count in ileum and cecum, thus improved the FCR of broiler chickens.

Keywords: Xylo-oligosaccharide, Glucose, Poultry diet, Gut microbiome, Intestinal bacterial count

Location: Chattogram Veterinary and Animal Sciences University, Khulshi-4225, Chattogram, Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Broiler

Objectives:

To investigate the effect of different levels of XOS on growth performance, blood biochemistry and intestinal bacterial count of broiler chickens.

Methodology:

All experimental procedures were approved by the CVASU (Chattogram Veterinary and Animal Sciences University) Ethics Committee (EC) and the EC Approval NO is CVASU/Dir(R&E) EC/2019/94(6). Dietary management: Four experimental diets were formulated with four levels; 0, 2.5, 5.0, and 7.5 g of XOS kgG¹ of diet, respectively. At first, a single batch of corn-soybean meal-based mashed diet was formulated according to the recommendation of the Cobb 500 Broiler performance and nutrition supplement guide to meet or exceed the nutrient requirements12. After that, the basal diet was divided into four aliquots according to the experimental diet arrangement. Each supplemental XOS level was mixed on top with each aliquot of the basal diet. The diets were made as mash and offered to the birds from day 13-26. The corn-derived XOS was purchased from Henan Heagreen Bio-technology Co., Ltd. (China) that contains <5% moisture, >95% XOS (as dry basis), <5% xylose, glucose and arabinose. Birds? managements and diets: A total of 96 Cobb 500 day- old broiler chicks (40±0.10 g) were purchased from a local hatchery and randomly distributed into four groups with four replicates per dietary group (6 birds/replicates). From day 1- 12, the birds received a basal (control) diet. The experimental diets were offered to the birds during 13-26 days of age. The birds were reared in cages with well-equipped feeders and drinkers. Birds had free access to feed and water. Standard vaccination schedules and management procedures were maintained throughout the trial period. Feed intake (FI) and live weight were recorded weekly. Mortality was recorded as it happened. Feed conversion ratio (FCR; feed intake/body weight gain) was corrected for mortality. Sample collection and processing: On day 26, three birds from each replicate were sacrificed by cutting the jugular vein after 12 h of fasting. The blood sample was collected in a falcon tube for separation of serum by centrifugation at 5000 revolutions per minute. Harvested serum samples were taken into the 2 mL Eppendorf tubes and stored at -20EC in the laboratory for further analysis. The weight of the visceral organs (liver, spleen, bursa, breast, gizzard, intestine and abdominal fat content) was recorded after opening the abdominal cavity of the same birds. The ileal (from the duodenum to Meckel's diverticulum) and cecal content were collected by gently pressing and stored in a separate labeled container at -20EC for further analysis. The weight of the different body parts of the dressed birds was recorded accordingly. Culture and total viable count: The collected intestinal samples of three individual birds/replicates were mixed and pooled. Around 1 g of ileal and caecal content was taken into two separate labeled sterile test tubes containing 2 mL of 0.9% saline solution with a stick. A 10-fold serial dilution was done for each pooled sample (0.1 mL) from 10-1 to 10-10. MacConkey agar, Violet red bile agar and KF streptococcus agar were used to enumerate the Enterobacteriaceae, Streptococci and Enterococci, respectively. Baird Parker agar and Mannitol salt agar were used for the enumeration of Staphylococci. All the plates were incubated at 37EC aerobically for 24-48 h and the number of colonies was counted accordingly13. Chemical analysis: The protein, CF, ash and a moisture percentage of the diet were analyzed by using the Association of Official Analytical Chemists method14. The nitrogen content of the samples was determined by the Kjeldahl method. The obtained nitrogen value was multiplied by 6.25 to convert it to crude protein. The weight difference methods were used to determine moisture and ash content levels. The crude fat of the diet was determined using the AOAC procedure with petroleum ether as a solvent. The serum glucose, triglyceride, total protein (TP), GPT (glutamic pyruvic transaminase), GOT (glutamic oxaloacetic transaminase), cholesterol, creatinine, T₃ (triiodothyronine) and T₄ (thyroxine) level was analyzed by using their respective standard assay kit (Randox Laboratories Ltd, UK) and semi-automated Humalyzer (Humalyzer 4000 Merck, Germany). Statistical analysis: Data was analyzed using one-way ANOVA. Differences between means were tested by the least significant difference (LSD) using SPSS v.16 statistical software (SPSS, Chicago, Illinois, USA) for windows. The linear and quadratic responses of dependent variables to dietary supplemental XOS levels were assessed by using orthogonal polynomials. The difference between means was considered significant at pc0.05.

Source of Information: International Journal of Poultry Science; 20 (4): 158-164, 2021

Article Link (Online Journal): DOI: 10.3923/ijps.2021.158.164

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study indicated that dietary supplementation of 2.5 g XOS kgG¹ significantly improved the FCR and TVC in ileum and caecum. The diet containing 2.5 g XOS kgG¹ decreased the serum glucose but increased the T₃ and T₄ levels. Moreover, the inclusion of XOS (2.5 g kgG¹) into the diet increased the dressing percentage but decreased the abdominal fat content of broiler chickens. In brief, XOS supplementation can improve the overall performance of broiler chickens when supplemented at a dose of 2.5 g XOS kgG¹.

Knowledge Management: Journal