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Effect of Semen Type, Breed Difference and Seasonal Variation on the Conception Rate of Cows in Artificial Insemination

S. S. Islam
Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh

S. C. Dash
Scientific Officer
District AI Centre, Khulna, Bangladesh

A. Ashraf
Biotechnology Discipline, Khulna University, Khulna 9208, Bangladesh

Abstract/ Executive Summary:

The experiment was carried out during August 1996 to December 1998 to determine the conception rate (CR) in artificial insemination (AI) using liquid and deep frozen semen. The effects of season and breeds of cows on CR were also considered. Out of 184 cows and heifers inseminated, 62 were Local, 64 were Local 1. Friesian and 58 were Local 2. Sahiwal crossbreds. Sixty-one (61) and 123 cows and heifers were inseminated by liquid and deep frozen semen respectively. Liquid semen was produced locally at district AI center, Khulna and deep frozen semen was collected from central cattle breeding station, Savar, Dhaka. Average motility of sperm in liquid and deep frozen semen was 70% and 60% respectively. AI was done by recto-vaginal method between 8 to 24 hours after onset of estrous and pregnancy diagnosis of all non-returned animals was done between 60 to 90 days after insemination by rectal palpation method. The animals were reared under semi-intensive condition. Data were analyzed by statistical package ‘MINITAB’. Analysis of variances revealed a significant effect (P<0.05) of semen type (liquid or deep frozen) on CR. Mean CR was found 65.57 + 6.13% and 49.59 + 4.53% for liquid semen and deep frozen semen respectively and the overall rate was 54.89 -3.68%. On the other hand, average conception rate in Local (indigenous zebu type), Local x Friesian and Local x Sahiwal were 58.06 + 6.32%, 46.88 + 6.29% and 60.34 + 6.48%, respectively. Seasonal variation in CR was insignificant (P>0.05).

Keywords: Artificial insemination; Cow; Conception rate; Pregnancy; Bangladesh

Location: Daulatpur, Khulna

Start Date: 00-08-1996

End Date: 00-12-1998

Program Area: Animal Health and Management

Commodity: Cow

Objectives:

The present study was undertaken to determine the effects of semen type, breeds of cow and season of AI on conception rate. Little work has been done on AI in this region, therefore the present work is of great importance.

Methodology:

The study was conducted on a selective area at Daulatpur, Khulna during August 1996 to December 1998. Artificial insemination (AI) was carried on 184 cows and heifers taken at random.

Semen used for AI: Both liquid and deep frozen semen were used for AI. Liquid semen was produced from four crossbred bulls of Khulna District AI centre. Collected semen was diluted with “egg yolk citrate” dilutor. Sperm concentration was kept 20 to 25 million per ml and average motility was 65 to 70%. AI was done within 48 to 72 hours of preservation and semen was kept at 50C before AI. In this experiment 61 cows and heifers were inseminated with liquid semen. Deep frozen semen was supplied from Central Cattle Breeding Station, Savar, Dhaka. The number of motile spermatozoa per dose (0.25ml) was 30 million and average motility was 60%. A total of 123 cows and heifers were inseminated with deep frozen semen. Insemination was done by rectovaginal method between 8 to 24 hours after onset of estrous.

Cows and heifers: A total of 184 cows and heifers were inseminated. Out of total inseminated animals, 38 were heifers and 146 were cows. The ages of the animals were between 2 to 12 years and the average was 5.20 years. Out of 184 cows and heifers, 62 were Local (indigenous zebu), 64 were Local x Friesian and 58 were Local x Sahiwal crossbreds. The animals were reared under semi-intensive condition. The animals were allowed to graze on natural grasses. Concentrate mixtures and rice straw were supplied in addition to grazing.

Season of AI: Year round AI programme was divided into three seasons, summer (March to June), monsoon (July to September) and winter (October to February) considering average ambient temperature, humidity and rainfall.

Statistical analysis: Data were analyzed by statistical package ‘MINITAB’. General Linear Model was used to analyze the effects of semen type, breeds of cows and season of AI on conception rate.

Source of Information: Khulna University Studies 2(2):

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It might be concluded here that the CR with liquid semen was higher than deep frozen semen. Proper preservation and handling of deep frozen semen as well as appropriate thawing techniques would enable to increase the efficiency of deep frozen semen. There were no significant effects of cow breeds and season of AI on CR.

Knowledge Management: Journal