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Effect of Different Media Additives on in Vitro Culture of Potato (solanum Tuberosum)

S.A.K.U. Khana*
Discipline of Agrotechnology, Khulna University, Khulna, 9208.

M.G. Rabbani
Department of Horticulture, Bangladesh Agricultural University, Mymensingh - 2202

Abstract/ Executive Summary:

Effect of different media additives on in vitro culture of three potato varieties viz.Lalpakri, Cardinal and BARI TPS-1 was investigated using single nodes as explant. The single nodes were collected from microplants of tuber sprouts that was preincubated under dark. Survivability of the cultures was statistically insignificant in respect of the media used, but the highest percentage of cultures survived on MS medium supplemented with 60 mg L-1 inositol. The time taken to initiate new shoots was minimum on MS medium supplemented with 1 mg L-1 Benzyle Amino Purine (BAP). The number of shoot was maximum on a medium containing MS + 0.25 mg L-1 Gibberellic Acid (GA3) and 2 mg L-1 Calcium Pantothenate. The maximum number of roots was obtained on medium containing MS + 0.03 mg L-1 Naphthalene Acetic Acid (NAA). Among the varieties tested, BARI TPS-1 performed better in respect of single node culture. The interaction between different potato varieties and media compositions was significant for different characters of in vitro grown plantlets.

Keywords: Growth rate, In vitro culture, Media additives, Growth regulator, Potato.

Location: Bangladesh

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Potato

Objectives:

The present study was undertaken with the view to find out the more effective vitamins and/or growth hormones as media additives for suitable in vitro culture of three potato varieties.

Methodology:

The present study was conducted to investigate the effect of different media additives, specially some vitamins and hormones on in vitro culture of three potato varieties viz., Lalpakri, Cardinal and BARI TPS-1. Potato tubers were collected from healthy and disease-free plants grown at the Horticulture Farm, Bangladesh Agricultural University, Mymensingh. Single node cuttings of three potato varieties, were used as explants. Single nodes were collected from microplants of tuber sprouts that was preincubated under dark.

Murashige and Skoog (MS) (1962) medium was used supplemented with 0.8% agar, 3% sucrose and with varying hormones and vitamins as indicated elsewhere. The pH of the media was adjusted to 5.8 prior to autoclaving at 121^oC for 20 minutes at 1.16 kg cm^-2. Potato sprouts of 0.5 ± 0.2 cm in length consisting of a single node were isolated from the microplants with sharp scalpel and the leaf attached to the node was excluded. Isolated single nodes were surface sterilized and inoculated aseptically to baby meal jars, each containing 20 ml of medium. The cultures were incubated in a growth room at a temperature of 25^o ± 2^oC, illuminated with white cool flourescent tubes (4.83 fc 84 TDL/Phillips). The room was illuminated 16 hours daily with a light intensity of 1500 lux.

Treatments: Different treatments of the experiment were as follows: I. Culture media T1 = MS + 1 mg L-1 BAP T2 = MS + 0.25 mg L-1 GA3 + 2 mg L-1 Calcium Pantothenate T3 = MS + 0.03 mg L-1 NAA T4 = MS + 60 mg L-1 inositol T5 = MS + 30 mg L-1 Adenine Sulphate.

II. Potato varieties: V1 = Lalpakri V2 = Cardinal V3 = BARI TPS-1

There were five replications for each treatment. Data on per cent survivability of cultures, time taken to initiation of new shoots and roots and their number, height of the plantlets (cm), number of leaves and nodes, root length (cm), growth rate (mm/d) and vigour of the plantlets were recorded. The vigour of the plantlets was measured on assumption within a scale of 1 to 10. The data for the characters under study were analysed and evaluated by F-variance test. The significance of differences between pair of means was evaluated by Least Significance Difference (LSD) test (Gomez and Gomez, 1976).

Source of Information: Khulna University Studies 2(2):

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Maximum number of nodes (17.37) was found in BARI TPS-1 x T2 (MS+0.25 mg-l GA3+2 mg-l calcium pantothenate) which might be due to the effect of GA3 and calcium pantothenate. The results of present experiment suggest that BARI TPS-1 is a promising hybrid with suitable multiplication qualities through in vitro culture and inositol supplemented medium can be used as a suitable medium for in vitro culture of potato.

Knowledge Management: Journal