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Preliminary Study on in Vitro Control of Bacteria Isolated from Macrobrachium Rosenbergii (de Man) Hatchery

S.J. Hossain
Biotechnology Discipline, Khulna University, Khulna-9208, Bangladesh

M.L. Saha
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

M. Ameen
Department of Zoology, University of Dhaka, Dhaka-1000, Bangladesh.

K. Azam*
Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh.

M.R. Khan
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

Bacterial species were isolated from the dead larvae and hatchery water of Backyard Freshwater Prawn (Macrobrachium resenbergii) hatchery at Noakhali, Bangladesh. The selected isolates were identified as Aeromonas sp., Bacillus badius, B. brevis, B. cereus, B. megaterium, Legionella sp., Micrococcus luteus, Moraxella Phenylpyruvica, Pseudomonas aeruginosa, Staphylococcus gallinarum and Streptococcus sobrinus. Formalin and antibiotics were used for invitro control of these bacteria. Three hundred ppm formalin effectively controlled the growth of all the selected species. Susceptibility test against the antibiotics showed that Gentamycin, Kanamycin, Streptomycin and Tetracycline should be the drug of choice to control bacterial flora of the hatchery.

Keywords: Bacteria; Prawn larvae; Hatchery; Formalin, Antibiotics

Location: Khulna regions

Start Date:

End Date:

Program Area: Pest Management

Commodity: Prawn

Objectives:

The present study was initiated to find out the suitable inhibitor of prawn hatchery’s bacteria that could be used in hatchery.

Methodology:

Dead larvae of M. rosenbergii and water of larval rearing tanks were collected from the hatchery. Bacteria associated with the water samples and macerated larvae were isolated by pour-plate method using nutrient agar media. The selected isolates were identified according to Bergey’s Manual (Krieg and Holt, 1984). Susceptibility of the isolates against formalin (BDH Chemicals Ltd., Poole England, Formaldehyde solution 37 to 41% HCHO) was determined by tube dilution technique. Nutrient broth consisting different concentrations of formalin viz. 0, 10, 20, 30, 50, 100 and 200 ppm were used. In case of antibiotics, commercially available antibiotic-impregnated discs were used.

Source of Information: Khulna University Studies, Vol. 1, Issue 2, December 1999, pp. 267-271

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The probable cause of mass mortality in prawn hatchery may be attributed to bacteria with particular reference to filamentous type. Three hundred ppm formalin was found to be effective in inhibiting all the selected bacterial species. Appropriate dose of formalin un-affecting the prawn larvae was not determined. Effective antibiotics should be used to avid mass mortality in prawn hatchery.

Knowledge Management: Journal