Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Haploid Plantlets in Anther Culture of Wheat

Md. Monirul Islam*
Discipline of Agrotechnology, Khulna University, Khulna-9208, Bangladesh

G. I. Ivanov
Department of Biotechnology, Kuban State Agrarian University, Krasnodar, Russia.

Mahtalat Ahmed
Department of Biotechnology, Kuban State Agrarian University, Krasnodar, Russia.

Abstract/ Executive Summary:

The experiment was conducted to assess the effect of different concentration combination of 2,4D, a-NAA and kinetin on callus induction and regeneration of haploid in anther culture of wheat. Potato-2 liquid medium supplemented with different concentration combinations was used for this purpose. Among all the formulations, efficient callus induction and plant regeneration were observed in liquid potato medium containing following concentrations (mg l-1) of plant growth regulators: 2,4D-0.75; a-NAA-0.1 and kinetin 0.75. This formulation supported to produce haploid plantlets directly without transferring calli to regeneration medium.

Keywords: Wheat; Anther culture; Callus induction; Regeneration; Growth regulators

Location:

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Wheat, Growth regulator

Objectives:

The success is restricted by two main factors; low frequency of callus induction and regeneration of plants. Medium composition, including organic and inorganic elements and growth regulators, genotype of the cultivars and culture environment after inoculation are the important factors of anther culture. The aim of this study was to optimize the medium composition, to increase the efficiency of callus induction and plant regeneration in wheat anther culture.

Methodology:

The research work was carried out in 1991 at the laboratory of cell Engineering, Lukyanenko Agricultural Research Institute, Krasnodar, Russia. The Russian spring wheat variety ‘Drudzina’ was taken as a donor plant for anther. The donor plants were grown in the field having plot size 3m2 and maintaining the density at the rate of 500 plants per square metre. The following growth regulators were tested in the potato-2 medium for callus induction: 2,4D, a-NAA and Kinetin. Young spikes still enclosed in leaf sheaths were collected in the morning hours (8 to 9 am) and were placed in a bucket with water and kept for 7 days in a cool room at a temperature of 3^o to 5^oC. After the completion of chill treatment, the spikelets were isolated and their sterilization was done with 0.1% solution of sodium hypochlorite for 15 minutes, followed by washing them with autoclaved distilled water under aseptic condition. Inoculation of anthers was done under laminar airflow with the help of forceps in petridishes containing 25ml liquid medium. Fifty anthers were inoculated in a dish. Then the cultures were kept in a dark room at 26^oC for one month and after that the cultures were incubated in growth chamber maintaining temperature 22^oC at day time and 18oC at night in a 16 hour daylight regime at about 10,000 lux. According to Table 1 and Table 2 a total of 27 different combinations of 2,4D, a-NAA and kinetin were used in this experiment for callus induction and to realize their subsequent effect on plant regeneration. Each combination consisted of 3 replications and 50 anthers were inoculated per replication. After 45 days of inoculation of anthers, calli were subcultured on Murashige and Skoog medium supplemented with 2, 4D – 0.25 mg l-1 , a-NAA- 0.25 mg l-1 , kinetin – 0.5 mg l-1 , sucrose – 3% and agar – 0.7% with pH – 5.8 for regeneration. Efficiency of callus induction and regeneration of plants was determined by the following formulae:

1. Frequency of callus induction (%) = Number of anthers producing callus / Number of inoculated anthers x100

2. Frequency of plant regeneration (%) = Number of regenerate d plants / Number of plated calli x 100

3. Frequency of green plant regeneration (%) = Number of regenerated green plants / Number of plated calli x 100

4. Frequency of albino plants regeneration(%) = Number of regenerated albino plants / Number of plated calli x 100

Source of Information: Khulna University Studies, Vol. 1, Issue 2, December 199, pp. 273-276

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present work has shown that the effects of combined use of two auxin, 2,4D and a-NAA and one cytokinin, kinetin at the concentration of 0.75 mg l-1 , 0.10 mg l-1 and 0.75 mg l-1 respectively were superior for both callus induction and regeneration of green plants in wheat anther culture, particularly for the variety ‘Drudzina’.

Knowledge Management: Journal