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Research Detail

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M. A. M. Yahia Khandoker
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Tasmina Akter
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Prem A/L S. Ananthan
Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Malaysia

The aim of this study was to evaluate the morphological and histomorphological attributes of different stages of follicular population of bovine ovaries. Total twelve ovaries were collected from Brahman cows and classified as ovary with corpus luteum (CL) and ovary without CL group. Among the ovaries, 75% having no CL and 25% having CL. The mean weight of ovaries with CL and ovaries without CL were 8.47±0.38 and 8.19±0.20 g, respectively. The number of visible follicles on the surface of the ovaries without CL (26.04±0.19) were significantly (P<0.01) higher than that of ovaries with CL (23.00±0.08). Histological study further reveals that total number of follicles (198.10±0.46) and number of primordial (93.50±0.62), primary (69.40±0.36) follicles significantly (P<0.01) higher in ovaries without CL than those of ovaries with CL (170.14±0.56 and 76.53±0.40, 61.37±0.62), respectively. Besides this, ovaries without CL contains greater number of normal follicles (8.37±0.09) that having well-defined granulosa cell integrity and lower number of abnormal follicles (26.50±0.61) compared ovaries with CL (4.07±0.09;31.53±0.38). This study inferred that presence of CL may have negative impact on ovarian follicular development. Therefore, it is possible to obtain a higher number of normal quality follicles from the ovary devoid of CL for bovine biotechnological research.

  Bovine ovaries, Corpus luteum, Follicular population, Histology
  The Anatomy and Physiology Laboratory, Faculty of Sustainable Agriculture, University Malaysia Sabah (UMS) of Sandakan campus, Malaysia
  00-08-2016
  00-10-2016
  Animal Health and Management
  Cattle

To identify the Qualitative Evaluation of Slaughterhouse Bovine Ovaries by Histological Study.

The experiment was conducted at the Anatomy and Physiology Laboratory, Faculty of Sustainable Agriculture, University Malaysia Sabah (UMS) of Sandakan campus from August 2016 till October 2016. Collection and processing of ovaries: The ovaries were collected from the Brahman cows which have been culled and slaughtered at local slaughterhouse. The excessive tissues had been trimmed out from the ovaries and placed in the falcon tube contained 35% of formaldehyde solution at room temperature and transported to the laboratory immediately. Measurement of weight of ovary and follicle counting: The ovaries were then transferred in the sterilized petridish containing same solution (35% formaldehyde) at room temperature and categorized on the basis of the presence or absence of corpus luteum and recorded. The weight of the ovary was measured individually by using electronic balance and recorded in a tabular form. In each and every ovary, the numbers of follicles on the surface of the ovaries were counted carefully by that visible on naked eyes. Histological sample preparation: The ovaries were cut into small pieces with surgical blades and forceps then fixed into the container (falcon tube) containing chemical mixture that composed of 37- 40% formaldehyde and glacial acetic acid for 24 hours or long until further treatment. Dehydration of tissue sample was carried out by upgrading the alcohols to absolute alcohol (50%, 70%, 80%, 95%, 100%) and cleaned with the clearing agent (xylene or chloroform). Thereafter, ovarian parts were transferred into a tube containing 50% paraffin and 50% xylene and placed the whole in the oven set at temperature about 50-60°C for 2 hours and embedded with melted pure paraffin and kept for hardening. Section cutting and staining The blocks were cut by using sliding microtome at 6 µm thick and placed in floatation water bath (55-56 °C) soaked with gelatin at every 10th section picked upon glass slide and dried in air before staining. The section on the slides was dehydrated with graded ethanol, stained with hematoxylin and cleaned in xylene and finally mounted with DPX and covered with the slip. Microscopy and histoplanimetry The prepared samples examined under microscope for identification of granular cells and other follicles. The follicles were counted by using microscope at the magnification of 40x.The different stages of the follicles also been taken into count to make some correlated results (Akers and Denbow, 2008, Rodger and Irving, 2010, and Cushman et al.,2009). Besides that, based on the cell integrity of the ovaries number of normal and abnormal follicles were counted using microscope at a magnification of 100x with oil drop. Statistical analysis The data was compiled, tabulated and analyzed based on the objectives of the study. The data was analyzed with the help of Statistical Analysis System (SAS) software version 9.1.3 (SAS, 2009). All data were subjected to one-way ANOVA and the statistical significance of difference between the groups was evaluated by Duncan’s multiple range test (DMRT). Pearson’s correlation was performed to compare among different types of follicular correlation with regards to presence or absence of CL in ovary. 

  J Bangladesh Agril Univ 19(2): 000–000, 2021; ISSN 1810-3030 (Print) 2408-8684 (Online)
  https://doi.org/10.5455/JBAU.54052
Funding Source:
1.   Budget:  
  

From the present study, it is concluded that ovaries without CL contributing not only higher number of follicles but also greater number of normal follicles that having good quality granulosa cell integrity compared to ovaries with CL. Hopefully, this finding will provide baseline information for further studies to improve livestock reproductive efficiency in Malaysia.

  Journal
  


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