Dilruba Yesmin
Institute of Environmental Science, University of Rajshahi, Rajshahi-6205, Bangladesh
Md. Monirul Islam
Institute of Environmental Science, University of Rajshahi, Rajshahi-6205, Bangladesh
Md. Abul Kalam Azad*
Institute of Environmental Science, University of Rajshahi, Rajshahi-6205, Bangladesh
Wheat, Plant extracts, Seed pre-treatment, Germination, Yield, Fungal disease
Botanical Pesticides Research Field of the Institute of Environmental Science, University of Rajshahi, Bangladesh
Pest Management
Wheat, Fungal Disease, Extract (plant, seed)
2.1.Plant Materials Plants having the bioactivity against the fungal diseases were surveyed through survey of literature and published papers. Thereafter, a field survey was conducted to identify the origin, habitat and status of these plants in the campus of University of Rajshahi. Ten most bio-active plants were selected to verify the activity against fungal pathogens of wheat. The collected plants are Turmeric powder (Curcuma longa), Indian Gum Leaf (Acacia nilotica), Bael Leaves (Aegle mormelas), Chirota (Swertia chirota), Allspices (Pimenta dioica), Turmeric Leaves (Curcuma longa), Lemongrass (Cymbopogon citratus), Khoksha Leaves (Ficus glomerata Roxb.), Dry Henna Leaves (Lawsonia inermis) and Fresh Henna Leaves (Lawsonia inermis).
2.2.Plant Extract and Seed Pre-treatment About 100 gram of plant material was dissolved in 1000 ml distilled water (w/v, 1:10) and kept in room temperature for 2-3 days, and then passed through two layers of cheese cloth (Hossain and Hossain, 2012). The extracts were centrifuged at 3000 rpm for 20 minutes and stored in a refrigerator at 4°C until used. Wheat seeds were treated with the prepared plant extracts separately by dipping method for 30 minutes. The treated seeds were soaked on blotting paper and sown in the experimental field to determine seed germination and other yield contributing character.
2.3.Experimental Plot and Treatments This field experiment was conducted at Botanical Pesticides Research Field of the Institute of Environmental Science, University of Rajshahi, Bangladesh during Robi season of 2017-2018. Wheat variety Pradip was sown on 15th November, 2017 in the experimental field. Ten plant extracts were used against fungal pathogens of wheat.
The experiment was laid-out under three replications following eleven (11) treatments (T). These are control (without plant extracts), T1 – turmeric powder extract, T2 – babla leaves extract, T3 –bael leaves extract, T4 – chirota leaves extract, T5 – allspices extract, T6 – turmeric leaves extract, T7 – lemongrass extract, T8 – khoksha leaves extract, T9 – dry henna leaves extract and T10 – fresh henna leaves extract.
Rajshahi University Journal of Environmental Science, 7, 54-59, 2018
Journal