Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Histological Changes in Some Tissues of Freshwater Crab (paratelphusa Lamellifrons) Exposed to Pesticides

Md. Jahurul Haque
Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

Md Moniruzzaman Sarkar
Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

Md Badrul Islam*
Drugs & Toxins Research Division, BCSIR Laboratories, Rajshahi, Rajshahi 6205, Bangladesh

Md Redwanur Rahman
Institute of Environmental Science, University of Rajshahi, Rajshahi 6205, Bangladesh

Lailatul Ferdousi
Applied Zoology Research Division, BCSIR Laboratories, Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

The present study was conducted to evaluate the effect of two pesticides Morter 48 EC (Chlorpyrifos) and Virtako (mixture of Chlorantraniliprole and Thiamethoxam) on freshwater crab Paratelphusa lamellifrons (Alcock, 1900) in the aquarium exposed under controlled conditions. These types of pesticides are applied widely in agricultural field. For histological studies, P. lamellifrons were exposed to sub lethal concentrations (0.2ppm, 0.4ppm, 0.8ppm) of Morter 48 EC and (3.75ppm, 7.5ppm, 15ppm) of Virtako respectively for 7 days. In the present study dose dependent mortality and several histological alterations (like vacculation and fragmentation etc.) were observed in the tissue of ovaries, testes, gills and stomach of freshwater crab P. lamellifrons. Hyperplasia, swelling, rogue were noticed in the ovary, testes, gill and stomach of Morter 48EC and Virtako treated crabs. Mortality rates ranged between (50-90)% with LD50 value 0.25 ppm for Morter 48EC, whereas (10-50)% for Virtako with LD50 value 16.00 ppm. Higher mortality rates, as well as histological changes, were observed for Morter 48EC compared to Virtako pesticide. The histological changes thus observed for both the pesticides in ovary, gills, testes and stomach of P. lammellifrons might be used as pesticides (Morter 48EC and Virtako) exposure indicators.

Keywords: Freshwater crab, Pesticide, Histology, Tissue, Histological changes.

Location: Rajshahi University campus of the Rajshahi City, Bangladesh

Start Date: 00-09-2013

End Date: 00-12-2013

Program Area: Risk Management in Agriculture

Commodity: Pesticide, Crab

Objectives:

Paratelphusa lamellifrons is a very common freshwater crab in our country which found almost all aquatic ecosystem including paddy field (Roy, 2011). Very limited or no work has been done on the histological effects of Morter 48 EC and Virtako on freshwater crab (Paratelphusa lamellifrons). Hence, the present study was undertaken to study the effects of Morter 48 EC and Virtakoon histological change in some tissues of the organ of P. lamellifrons.

Methodology:

2.1. Experimental Animals Adult fresh water crab Paratelphusa lamellifrons (carapace length ranging from 25 to 30 mm, breadth ranging from 30 to 45 mm and body weight ranging from 15-20g) were captured from the swallow lands of Rajshahi University campus of the Rajshahi City, Bangladesh, during the period from September to December 2013, by using cast net, and were immediately transported to the laboratory in porous plastic container. The crabs were acclimatized in the laboratory condition for two days. The crabs were fed with flour paste once each day. Then the crabs were immediately (after two days) transferred into the experimental aquarium.

2.2. Selection of Pesticides Two commonly used pesticides such as Morter 48 EC (Chlorpyrifos) and Virtako (mixture of Chlorantraniliprole and Thiomethoxam) were chosen for this experimental work.

2.3. Median Lethal Concentration (LC50) The acute toxicity tests were conducted in duplicates using 10L experimental aquarium. The duration of the test was 7 days and during the study the experimental crabs were fed. A minimum of 3L water was added for 10 crabs, so that the crabs were fully immersed. The water was renewed each day to avoid depletion of dissolved oxygen in the medium. The mortality of P. lamellifrons was recorded every 24h exposure to pesticides. The LC50 values were obtained by probit analysis (APHA, 1992). Median lethal concentration (LC50) for 24h exposure period of Chlorpyrifos 0.2 ppm, 0.4 ppm and 0.8 ppm and Virtako 3.75 ppm, 7.50 ppm and 15.00 ppm were estimated.

2.4. Experimental Design The effect of pesticides on P. lamellifrons was conducted by exposing the crabs (7 groups containing 5 crabs in each group) to sublethal concentrations Chlorpyrifos 0.2 ppm, 0.4 ppm and 0.8 ppm and Virtako 3.75 ppm, 7.50 ppm and 15.00 ppm respectively for 7 days. One group (containing 5 crabs). was treated with tap water as control group. After treatment period the Control and treated crabs were sacrificed. Ovary, gill, testes and stomach were collected and put in aqueous Bouins fluid. After fixation for 24-30 h, tissues were dehydrated through a graded series of ethanol, cleared in xylene, and infiltrated in the paraffin. Sections of 4-6 μm were prepared from paraffin blocks by using a rotary microtome. These sections were then stained with Hematoxylin-Eosin. Histopathological lesions were examined and photographed using microscope (Olympus, Japan, CH4O) with photographic attachment camera.

2.5. Statistical Analysis The data was statistically analyzed using SPSS software. Regression and Analysis of variance (ANOVA) were used to determine the significant difference among the pesticides.

Source of Information: Rajshahi University Journal of Environmental Science, 6, 55-63, 2017

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Pesticide pollution in agriculture field and aquatic ecosystem is a major environmental concern because it subsequently affects all living organisms in the ecosystem. The histological studies not only give an early indication of pollution hazard, but also provide useful data on nature and degree of damage to cells and tissues. It is a common tool for determining the deleterious effects of toxic substances in animals. According to this study we can say that Virtako is one of the eco-friendly pesticides, whereas the use of Morter 48 EC in various agriculture fields should be control.

Knowledge Management: Journal