Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Comparative Studies on Detection and Quantification of Pesticide Residue in Some Vegetables of Bangladesh

Shamsun Naher*
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh

Md. Aminul Haque
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh

Md. Shahid Alam
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh

Mohammad Mostafizur Rahman
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh

Md. Delwar Hossain
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh

Mala Khan
DRiCM, BCSIR laboratories, Dr. Qudrat-I-Khuda Road, Dhaka-1205, Bangladesh

Abstract/ Executive Summary:

The prevalently used pesticide Malathion E50, Alpha-Cypermethrin, Chlorpyrifos and Lambda-Cyhalothrin residues were identified and quantified in four vegetable samples (Brinjal, Coli flower, Lady’s finger and Bean) collected from three different places (Kadamtoli, Alipur and Kanarchor) of Bangladesh. The data were compared to the data of one of our previous article (Naher et al., 2016) data for another set of similar investigation for the same pesticide residues. QuEChERS method was used for extraction and Gas Chromatography-Mass Spectrometry (GCMS) was used for detection as well as for quantification of the pesticides. From the comparison of data, it was concluded that leafy vegetables investigated in our previous work contained a comparatively higher amount of pesticide residues than the four vegetables investigated in our present work. Relatively higher amount of pesticide residues were obtained in some samples. However, most of the samples were found to contain moderate to low concentration of pesticide residues. The detected pesticide concentration exceeded the Maximum Residue Limit (MRL) in some samples which is obviously harmful and alarming for sound human health of the people for whom it was marketed.

Keywords: Leafy vegetables; Gas Chromatography-Mass Spectrometry; QuEChERS method; MRL; Chlorpyrifos; Lambda-Cyhalothrin.

Location: Hazratpur at Keraniganj in Dhaka

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Pesticide, Vegetables

Objectives:

In our present work we have investigated for four different widely used pesticides in four different vegetables collected from three different vegetable fields of Keraniganj near Dhaka, Bangladesh and compared the data with our previous work.

Methodology:

Study area: The selected area of study was Hazratpur at Keraniganj in Dhaka. There were various vegetable fields which grown large amount of vegetables that satisfy the vegetable demand of local people as well as Dhaka city people. Four different samples from each of the three different places namely Kadamtoli, Alipur and Kanarchor of Keraniganj were collected and analyzed for widely used different pesticide residue.

Sample collection: Samples were collected from three different places in Hazratpur. Four different samples were collected from three different fields. These were Lady’s Finger (Vendi), Cauliflower (Fulcopi), Bean (Shim), and Brinjal (Begun).

Chemicals: Chemical reagents and solvents that used in the study were namely Acetonitrile (MeCN), Magnesium Sulphate (MgSO4), Primary and Secondary Amines (PSA), Acetic Acid (HOAc), Sodium Acetate (NaOAc), Methanol (MeOH) etc.

Analytical Instrumentals: Gas Chromatography-Mass Spectrometry (GC-MS).

Pesticides Extraction method: QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe).

Experimental: QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method of pesticides extraction was used in this experiment. In this method, at first about 200 g sample was taken and it was chopped with vertical cutter. Then the chopped sample was grinded with mortar and pestle to homogenize the sample. The homogenized sample (15 g) was transferred to Teflon tube (50 mL). Then a solution of acetic acid (1%) in acetonitrile (15 mL), anhydrous sodium acetate (1.5g) and anhydrous magnesium sulphate (6 g) was added to the sample. The mixture was then shaken vigorously, vortexed and centrifuged (~1500 rcf) for one minute each. A portion of MeCN extract (5 mL) (upper level) was collected from the mixture, and then anhydrous MgSO4 (150mg) and PSA (250 mg) were added the extract. The extract with MgSO4 and PSA was shaken in the vortex for 30 s and was filtered. About 1.0-1.5 mL of the extract was transferred into GC vial filtering through 0.45 µm syringe filter. Finally, the extract was analyzed by GC-MS (AOAC International, 2005). Using this procedure, pesticide residues were extracted and analyzed from all the 12 samples.

Source of Information: Jagannath University Journal of Science, Volume 6, Number I & II, 2019 ISSN 2224-1698

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In our present study, some vegetable collected from some places contained little or no pesticide residue. Some samples however contained moderate to high amount of different pesticides. Brinjal of any of the three selected areas under investigations contained no pesticide residue. However, the detected amount of pesticides found in this investigation which was used in vegetables such as Cauliflower, Lady’s Finger and Bean were higher than that of permissible limit and hence those were harmful for the consumers. So, proper assessment, rigorous monitoring and environmental implications of synthetic chemicals should be ascertained well before allowing for large scale use. More significantly, the long-term implications on the human health and environmental need should be studied significantly for sustainable development. Government of Bangladesh should take proper action to assess the pesticide management and to regulate the use of pesticides in crops, especially in vegetables.

Knowledge Management: Journal