M. RAHMAN
Pulses Research Centre, RARS, BARI, Ishurdi, Pabna
M.A. HOSSAIN
Pulses Research Centre, RARS, BARI, Ishurdi, Pabna
M.E. ALI
Pulses Research Centre, RARS, BARI, Ishurdi, Pabna
M.A.H. BHUIYAN
Pulses Research Centre, RARS, BARI, Ishurdi, Pabna
Rhizobium, Mungbean, Arbuscular mycorrhiza
Crop-Soil-Water Management
Bio fertilizer
The experiment was carried out during rabi season from March 2017 to May 2017 in the net house of Soil Science Division, BARI, Joydebpur, Gazipur (230 59'378'' N latitude, 900 24'886'' E longitude and 8.4 m elevation). Seeds of mungbean (BARI Mung-6) were collected from Pulses Research Centre, BARI, Gazipur. The silted (sandy clay loam) soils were collected from the bank of Turag River at Kodda, Gazipur mixed with cowdung at 5:1 ratio and was used as the potting media. Each pot (28 cm in diameter and 23 cm in height) was filled with approximately 8-kg of soil leaving the upper 3 inches of pot was vacant to facilitate watering. The pH of cowdung was 6.7 and the nutrient contents were: organic matter 14.1%, N 0.8%, P 1.26%, K 0.88%, Ca 1.55%, Mg 0.82%, S 0.62%, Fe 0.25% and Mn 0.112%. The physical and chemical properties of the soil were analyzed. The soil contained 12 AM (100-1 g soil) spores of indigenous mixed AM fungal species and the experiment was conducted under unsterilized soil conditions. Soil pH was measured by a combined glass calomel electrode (Jackson, 1958). Organic carbon was determined by the Wet Oxidation Method (Walkley and Black, 1934). Total N was determined by the modified Kjeldahl method (Jackson, 1962). Calcium, K and Mg were determined by the NH4OAc extraction method (Black, 1965). Copper, Fe, Mn and Zn were determined by DTPA extraction followed by AAS reading. Boron was determined by CaCl2 extraction method. Phosphorus was determined by the Modified Olsen method (Neutral + Calcareous soils) according to Olsen et al. (1954). Sulphur was determined by CaH4(PO4)2.H2O extraction followed by turbidimetric turbidity method with BaCl2. Chemical fertilizers @ 22.56 kg N ha-1 from urea, 24.45 kg P ha-1 from TSP, 34.27 kg K ha-1 from MoP, 7.95 kg S ha-1 from gypsum, 2.23 kg Zn ha-1 from ZnSO4, 0.81 kg B ha-1 from Boric acid and 0.34 kg Mo ha-1 from Na2MoO4.2H2O were applied (BARC, 2012). Half of urea-N and all other fertilizers were applied as basal during final land preparation and the remaining half amount of urea-N was applied after 10 days after sowing. The experiment was designed in RCBD with 10 treatments and 4 replications. Ten seeds were sown in each pot at 1 cm soil depth. After collecting germination percentage data the treatment was sustained with 6 vigorous seedlings in the mycorrhizal and non-mycorrhizal pot, and the other seedlings were removed from the pot. Then after collecting growth parameters and modulation data 3 plants were left finally in each pot for yield and yield contributing data. There were ten treatments viz. T1: Control, T2: Arbuscular mycorrhiza (AM) + 50% P, T3: AM + 75% P, T4: AM + 100% P, T5: Rhizobium + 50% P, T6: Rhizobium + 75% P, T7: Rhizobium + 100% P, T8: AM + Rhizobium + 50% P, T9: AM + Rhizobium + 75% P and T10: AM + Rhizobium + 100% P. All the AM spores were isolated from the extract with the help of a fine forceps into a watch glass with a small quantity of water. The extract, with AM spores, was observed under the stereomicroscope and the number of spores was counted. Spore numbers from the three replicates per sample were averaged and the result was expressed as a number per 100 g of dry soil basis. Assessment of root colonization infection: The percentage of AM infection was estimated by root slide technique (Read et al., 1976). One hundred root segments were examined for each sample. The stained root pieces were mounted in acidic glycerol on slides and the coverslip was placed and slightly pressed. The roots were observed under a microscope. A root segment was considered as positively infected if it showed mycelium, vesicles and arbuscules or any other combination of these structural characteristics of AM infection. The presence or absence of infection in the root pieces was recorded and the percent infection was calculated. Data were statistically analyzed using Analysis of Variance (ANOVA) following the CropStat package while the all pair comparisons were done by Statistix 10.
Annual Research Report 2016-2017, Pulses Research Centre, RARS, BARI, Ishurdi, Pabna
Report/Proceedings