Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Inhibitory Activity of Methanolic Extract of Araucaria Heterophylla Leaves Against Gram Negative Bacteria

Sara Sadia
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Bidduth Kumar Sarkar
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Asma Jerin
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Muaj Ibne Sahid*
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Abstract/ Executive Summary:

This study investigated antibacterial activity of methanolic extract of Araucaria heterophyllaleaves against twogram-negative bacteria Salmonella typhi and Escherichia coli by disk diffusion method. At the initial investigation, the test sample extract did not show inhibitory activity against Staphylococcus aureus gram positive bacteria. So, in the next step observation was made against above mentioned gram-negative bacteria. Test sample was taken at a concentration of 500 µg/disk.A broadspectrum antibiotic Kanamycin (30µg/disk) was used as standard to get comparable antibacterial activity. Weak antibacterial activity was shown in the test sample against S. typhi and E. coli with zone of inhibition 8 mm and 10 mm respectively. Although Kanamycin showed antibacterial activity against S. typhi and E. coli with zone of inhibition 32 mm and 35 mm correspondingly.

Keywords: Araucaria heterophylla, Extraction, Antibacterial, Escherichia coli, Salmonella typhi, Antibiotic resistance, Zone of inhibition.

Location: Ranada Prasad Shaha University campus, Shitalakhya, Narayanganj

Start Date: 00-08-2019

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The results apparently showed the potentiality of the extract to inhibit both gram negative bacterial strain. So, the crude extract has weak antibacterial activity in terms of gram-negative bacteria. Based on test results, it can be said that A. heterophylla plant is not only a decorative plant but also possesses medicinal value. This study opens the gate to investigate both phytochemically and pharmacologically thereby exploring the medicinal potentials of A. heterophylla plant.

Methodology:

Leaves of A. heterophyllawas collected from Ranada Prasad Shaha University campus, Shitalakhya, Narayanganj, in August, 2019 and was identified by Bangladesh National Herbarium, Mirpur, Dhaka. DACB Accession no is 48435. After collection, the leaves were cleaned gently. Then all the leaves were allowed for shade drying in clean area. After that leaves were dried by oven for 24 hours at 400C to facilitate grinding process. When the leaves were dried properly it was ground to very small pieces by using a domestic grinder. Then leaf powder was ready for extraction. Approximately 300g of powder materials were soaked insufficient amount of methanol for about two weeks. The containers with its contents was sealed and kept for 7 days with frequent stirring. After vacuum filtration, the filtrate was evaporated to get dark green, crude methanolic extract. Approximately 15.6% extract came out from 300g plant. Crude extract was stored for further biological investigations. By using disk diffusion method, the antibacterial activity of plant extract was carried out. (Bauer et al., 1966). Each chemicals and reagents used in the study were of analytical grade. Selected gram-negative bacteria (Salmonella typhi, Escherichia coli) were sourced from BCSIR (Bangladesh Council of Scientific and Industrial Research). A few colonies (3 to 10) of the organism to be tested were collected with a wire loop from the original culture plate and introduced into a test tube containing 4 ml of tryptose phosphate or trypticase soy broth. To form a bacterial suspension of moderate cloudiness, these tubes were incubated for about a time span of 2 to 5 hours. The suspension was then diluted with distilled water to a density visually identical to that of a standard, prepared by adding 0.5 ml. of 1 percent BaCl2 to 99.5 ml. of 1 percent H2SO4 (0.36 N). For sensitivity plates large 15 cm Petri dishes were used with Mueller-Hinton agar (5 to 6 mm in depth). Before inoculation, plates were dried for about 30 minutes and were used within 4 days of preparation. With the help of a cotton swab, the bacterial broth suspension was spread evenly in 3 planes onto the surface of the medium. The excessive suspension was extracted from the swab by being rotated against the side of the tube before the plates were seeded. Standard Kanamycin (30 μg/disk), blank disk containing only solvent and test sample disk (500 μg/ disk) were prepared. After the inoculum had dried (3 to 5 minutes), the disks were placed on the agar with flamed forceps and gently pressed down to ensure contact. Within 30 minutes, plates were incubated. After overnight incubation, the zone diameters (including the 6-mm disk) were measured with calipers near the agar surface. A reading of 6 mm indicates no zone (Bauer et al., 1966).

Source of Information: Jagannath University Journal of Life and Earth Sciences, 5(2): 216-2219, 2019 ISSN 2414-1402

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results apparently showedthe potentiality of the extract to inhibit both gram negative bacterial strain. So, the crude extract has weak antibacterial activity in terms of gramnegative bacteria. Based on test results,it can be said that A. heterophyllaplant is not only a decorative plant but also possesses medicinal value. This study opens the gate to investigate both phytochemically and pharmacologically thereby exploring medicinal potentials of A. heterophyllaplant.

Knowledge Management: Journal