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Antibacterial Activity of Ethanolic Extract of Commelina Benghalensis L. Leaf

Asma Jerin
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Bidduth Kumar Sarkar
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Tanjila Jaman Monia
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Balayatun Nessa
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Juwel Rana
Department of Nutrition & Food Engineering, Daffodil International University, Dhaka-1207, Bangladesh

Subrato Kumar Barman*
Department of Pharmacy, Ranada Prasad Shaha University, Narayanganj-1400, Bangladesh

Abstract/ Executive Summary:

This research study was designed for evaluation of antibacterial activity of ethanolic extract of Commelina benghalensisL. leaves (ECB) against Gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria. Here, the dried ground leaves of C. benghalensis L. were extracted with cold ethanol and then was evaluated for the inhibitory activity (500 µg/disc) against S. aureusand E. coli through disk diffusion method using kanamycin (30 µg/disc) as standard. ECB showed potential antibacterial activity against S. aureusand no inhibitory activity against E. coli. To validate confirmation of found antibacterial activity isolation of bioactive principle(s) and further study need to be performed.

Keywords: Antibiotic resistance, Commelina benghalensis L., Ethanolic extract, Disk diffusion, Staphylococcus aureus, Escherichia coli

Location: Ranada Prasad Shaha University campus, Shitalakhya, Naryanganj

Start Date: 00-08-2019

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

Thus, this research study was aimed at evaluation of antibacterial activity of ethanolic extract of only leaf of C. benghalensisL. following disc diffusion method against E. coli and S. aureus. Kanamycin, a broad spectrum antibiotic (Quinn et al., 2013), was used as standard drug to get comparable antibacterial activity of ECB.

Methodology:

Leaves of CommelinabenghalensisL. were collected from Ranada Prasad Shaha University campus, Shitalakhya, Naryanganj on August, 2019 and then was identified & authenticated by Bangladesh National Herbarium (BNH). A voucher specimen was submitted to BNH for further reference. The accession number given by BNH is DACB48434.

Whole plants were collected and dedusted. Leaves were collected from the whole plant. Leaves were shade dried & ground to make coarse powders. At room temperature, approximately 150 g of crushed powder was soaked in 1000 ml of ethanol for ten days. The filtrate was evaporated to get crude ethanolic extract following vacuum filtration. Percentage (%) of yield was 12.67%.

Antibacterial assay was performed by disk diffusion method (Bauer et al., 1966). A few colonies (3 to 10) of the organism to be investigated were moved from an initial culture plate with a wire loop into a test tube containing 4 ml of tryptose phosphate or soy broth trypticase. To create a bacterial suspension of mild cloudiness, these tubes were then incubated for 2 to 5 hours (Bauer et al., 1966). With distilled water, the suspension was then diluted to a density visually equivalent to that of a standard which was prepared by adding 0.5 ml of 1 percent BaCl2 to 99.5 ml. of 1 percent H2SO4 (0.36 N). Large (15 cm) petri dishes with Mueller-Hinton agar were used for the sensitivity plate (5 to 6 mm in depth). The plates were dried for approximately 30 minutes prior to inoculation. With a cotton swab, the bacterial broth suspension was uniformly streaked on the surface of the medium in 3 planes. The swab was rotated against the side of the tube prior to inoculation to get rid of the excess suspension. Standard kanamycin (30 μg/disk) disk, blank disk containing only solvent and test sample disk (ECB 500 μg/disk) were prepared. After drying the inoculum (3 to 5 minutes), the disks were placed with flamed forceps on the agar and pushed down gently to ensure contact. Plates were incubated within 30 minutes. The inhibition zone was assessed after overnight incubation with a slide calipers near the surface of the agar.

Source of Information: Jagannath University Journal of Life and Earth Sciences, 5(2): 220-223, 2019 ISSN 2414-1402

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

No inhibitory activity had been observed against E. coli by ECB at 500µg/disk. ECB showed potential inhibition against S.aureus with zone of inhibition 10 mm at 500 µg/disk while standard Kanamycin inhibited a zone of 32 mm at 30 µg/disk. This activity could be potential for fighting antibiotic resistance. However, extensive research studies with various concentrations of ECB involving multiple strains & multiple antibiotics need to be performed.

Knowledge Management: Journal