Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Cytogenotoxic Effects of Acid Dye on Channa Punctatus (bloch)

Sabina Sultana
Professor
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

Fahmida Akhter
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

Sahadat Hossain
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

The aim of this present investigation was to assess morphological and behavioural changes and to evaluate the histological changes of liver, stomach and intestine of Channa punctatus (BLOCH) at different concentration of acid dye. The study was conducted during the period November 2016 to August 2017 at Fisheries Research Laboratory, Department of Zoology, University of Rajshahi, Bangladesh. 40%, 60% and 80% mortality were recorded at 0.07, 0.09 and 0.2ml/L concentration of acid dye. Histopathological changes were observed in the liver, stomach and intestine of the treated fish compared to control fish. The experimental fish showed- dilation of sinusoid, enlarged central vein, vacuolization of hepatocyte, hemorrhage and necrosis in liver; degeneration in the layer, acute depletion of epithelium, necrosis and hemorrhage in stomach; complete destruction of villi, mass depletion of epithelium, and the rapture in the layer in intestine. The study revealed toxic effects of acid dye on C. punctatus.

Keywords: Cytogenotoxic, Acid dye, Morphological, Behavioural, Channa punctatus

Location: Fisheries Research Laboratory, Department of Zoology, University of Rajshahi, Bangladesh.

Start Date: 00-11-2016

End Date: 00-08-2017

Program Area: Animal Health and Management

Commodity: Cat fish

Objectives:

The objective of this present study was to observe morphological and behavioural changes and to evaluate the histopathological changes of liver, stomach and intestine of C. punctatus at different concentrations of acid dye.

Methodology:

Study area and study period- The study was conducted at the Fisheries Research Laboratory of the Department of Zoology, University of Rajshahi, located at Sir Jagadish Chandra Bose building of the University. The research was performed during the period from November 2016 to August 2017. Collection & pre-exposure acclimation of experimental fish- Healthy and active specimens of freshwater fish, C. punctatus were collected as experimental fish from the fisherman of the fish landing center of Rajshahi. The live specimens were transported to the laboratory. Fishes were acclimatized for two weeks in laboratory conditions. The fishes were fed two times (8 am, 4 pm) in a day. The water medium used was changed twice a day. This helped to remove the unused feed, fecal matter and other unwanted particles. Dye collection, dose estimation and exposure to acid dye- To conduct this study, the acid dye was collected from a retailer shop in Rajshahi town. Doses of acid dye were estimated and the doses of acid dye were 0.07, 0.09 and 0.2ml per liter water. Each dose was measured by micropipette and mixed with aquarium water. Four glass aquaria (with 50cm, 30cm, 20cm) were taken and washed with detergent and 0.1% of potassium permanganate to make the aquaria germ-free. After acclimatization, the experimental fishes were transported to glass aquaria in the laboratory. For histopathological studies, the fishes were exposed to 0.07, 0.09 and 0.2ml acid dye per liter water in four glass aquaria separately. The experimental fishes were kept under observation for 21 days. The experimental conditions and mortality were observed. At the end of exposure, the survival and dead fishes used for histological study. A control aquarium was also maintained under laboratory conditions. Calculation of RPS (Relative percentage of survival) Relative percentage of survival (RPS) was calculated according to the following formula: RPS= (1- % mortality in treatment with dye / % mortality in control) × 100. Dissection and tissue preservation- The fishes were sacrificed by decapitation and dissected after the completion of exposures to the different concentrations of acid dye. The target organs were surgically removed and thoroughly washed in cold normal saline solution (0.85% NaCl). The tissue of target organs like intestine, stomach and liver were fixed in Bouin’s fluid and then they were processed by standard procedure to routine microtome technique. Tissue processing and slide preparation- The most commonly used method of studying tissues is done by preparing histological slides. The slide should be processed in a proper way so that it confidently reflects the real nature of the tissues of any specimen. The preserved tissues should go through a series of phases before observation. The total phases of preparing a permanent histological slide are given following ^[10-13]:

Fixation
Washing and Dehydration
Dealcoholizing
Infiltration
Embedding with paraffin
Trimming and block setting
Cutting paraffin ribbon
Stretching of small pieces of ribbon on the slides
Staining. Microscopic examination- The microscopic views of the tissues were taken at a resolution power with the help of a light microscope. All the slides were observed under low and high resolutions for their histological findings. Microscopic examination showed the histological abnormalities and differences from the control to the treated groups of different target organs of C. punctatus.

Source of Information: International Journal of Fisheries and Aquatic Studies; 2020; 8(5): 220-225

Article Link (Online Journal): www.fisheriesjournal.com

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The findings of this present study conclude that different concentrations of acid dye produced a different level of morphological, behavioral and histopathological changes in C. punctatus. Looking to the cytogenotoxic and histotoxic potential of acid dye, we should decrease the expose of acid dye in the environment through implementing essential accurate pre-treatment of wastewater from textile and dyeing industries before release. This study also recommends proper inspection of acid dye toxicity before using it in any industry. To save the fish population from threats, we should realize the injurious effects of acid dye and establish reliable wastewater treatment in the textile and dyeing industries.

Knowledge Management: Journal