The present research work was conducted in the experimental field of Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka during the period from November 2013 to April 2014 in rabi season. In this experiment 30 wheat genotypes were Akber, Kanchan, Sonalika, BARI Gom 20, BARI Gom 21, BARI Gom 22, BARI Gom 23, BARI Gom 24, BARI Gom 25, BARI Gom26, BD 478, BD 479, BD 481, BD 489, BD 492 , BD 7544, BD 7551, BD 7552, BD 7560, BD 7591, BD 7592, BD 7599, BD 7605, BD 7614, BD 7617, BD 7618, BD 7621, BD 7622, BD 7624, BD 7650. The purity and germination percentage of these seeds were leveled as 98% and 95%, respectively. These genotypes were collected from Wheat Research Centre (WRC) of Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur. Randomized Complete Block Design (RCBD) was used with 3 replications of the material. There were 90 unit plots altogether in the experiment. The size of the each plot was 2.0 m × 1.0 m. The distance maintained between two blocks and two plots were 1.0 m and 0.5 m, respectively. Seeds were sown continuously with maintaining 20 cm line to line distance and plant to plant 5 cm. Cowdung was applied @ 10 t ha-1 during 15 days before seeds sowing in the field. The entire amount of TSP, MoP and Gypsum, 2/3rd of urea (180, 50, 120, 220 kg/ha respectively) were applied during the final preparation of land. Rest of urea was top dressed after first irrigation (BARI, 2011). Fresh weight of grain was recorded plot wise from 1 m2 area. After harvesting the grains were dried, cleaned and weighed for individual plot. The weight was adjusted to a moisture content of 14%. Data was recorded on days to starting of heading, days to starting of maturity, plant height (cm), number of spikes/m2, number of spikelets/spike, number of grains/spike, chlorophyll content of 10 selected leaves, dry matter content, leaf area, peduncle length, root length, root number, weight of 1000 grains and grain yield per plant.
Statistical analysis: The mean values of all the characters were calculated and analysis of variance was performed. The significance of the difference among the treatment means was estimated by the Duncan Multiple Range Test (DMRT) at a 5% level of probability (Gomez and Gomez 1984). Genotypic and phenotypic variances, heritability were estimated with the help of the formula suggested by Johnson et al. (1955). Genetic advance and genetic advance in percentage of mean were estimated with the help of the formula of Allard (1960) and Comstock and Robinson (1952). Genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were calculated by Burton (1952). The simple correlation was estimated of the 14 traits with the following formula (Singh and Chaudhary 1985). Path coefficient analysis was done according to the procedure employed by Dewey and Lu (1959) also quoted in Singh and Chaudhary (1985) using simple correlation values.