Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Micro-propagation of Aloe Indica L. Through Shoot Tip Culture

Gokul Chandra Biswas
Department of Genetic Engineering and Biotechnology, School of Life Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

Md. Abunasar Miah
Department of Genetic Engineering and Biotechnology, School of Life Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

M. M. Hasan Sohel2 ,
2 (Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202,Bangladesh)

A. K. M. Shahadat Hossain
Bangladesh Jute Research Institute, Farm Division, Bangladesh

Shahriar Kabir Shakil
Department of Genetic Engineering and Biotechnology, School of Life Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

Moniruzzaman Sohag Howlader
Department of Genetic Engineering and Biotechnology, School of Life Science, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh

Abstract/ Executive Summary:

Shoot tip of Aloe indica L. variety was chosen as explants which disinfected with 2% NaOCl and washing thoroughly with sterile water. The shoot tip explants were placed on MS medium supplemented with Benzyladenin, Kinetin with various concentrations were 0.5 mg/L, 1 mg/L, 1.5 mg/L and 2 mg/L and Benzyladenin along in combination with Napthaleneacetic acid the concentration were 0.5mg/L + 0.5mg/L, 1mg/L + 0.5mg/L, 1.5mg/L + 0.5 mg/L and 2mg/L + 0.5mg/L. After 8 weeks, the best proliferation of average number of shoot per explants was 7.8 for the medium containing of 2 mg/l Benzyladenine with 0.5 mg/l Naphthaleneacetic acid and the lowest average number of shoot per explants was 0.9 for the medium containing of 0.5 mg/L Benzyladenine. For rooting, MS media supplemented with Napthaleneacetic acid, Indole-3-butyric acid acid and Indole-3-acetic acid with various concentration were 0.1 mg/L, 0.5mg/L, 1mg/L and 1.5 mg/L respectively were used. Highest average number of root per explants was 5.2 produced in 0.5 mg/L Napthaleneacetic acid concentration. On the other hand, the lowest average number of root per explants was zero with 0.1 mg/L Indole-3-acetic acid concentrations. Finally, the best shooting and rooting medium was identify MS medium in combination with 2mg/L Benzyladenin with 0.5 mg/L Napthaleneacetic acid and 0.5mg/L Napthaleneacetic acid respectively. Thus, this study could be ideal for rapid micropropagation of elite plants of Aloe indica L. By this protocol in future the cost of Aloe indica production will be decreased which will lead the decrease of aloe product prices.

Keywords: Aloe indica; Charcoal; In vitro culture; Micropropagation; MS medium; Shoot tip.I

Location: BCSIR Laboratory, Rajshahi, Bangladesh.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Shoot tip culture, Aloe Vera

Objectives:

For rapid multiplication of plants, tissue and organ culture technique is crucial tool now days. Although Aloe vera propagates vegetatively in its natural state, but propagation is too slow for commercial plant production [9]. To overcome slow propagation rate, micro propagation will be a very useful technique for mass multiplication of Aloe. The in vitro vegetative propagation has important benefits to produce stable lines in plants that have no named varieties e.g., Annona spp. and Australian dioecious papaw genotypes where traditional plant breeding has failed. It has also a great potential for the propagation of important crops like: Cassava spp., Phaseolus spp., Solanum spp., Worldwide there is much interest to promote the development of an in vitro technology that permits the propagation and breeding of commercial valuable woody, semi woody, ornamental, basic food, industrial and medicinal plants. Which species are in danger of extinction should receive a priority in terms of germplasm conservation.

Methodology:

Considering the medical importance and slower propagation rate, this micro propagation research work on Aloe indica L. was conducted in the BCSIR Laboratory, Rajshahi, Bangladesh. The detail of materials used and analytical methods employed during this study is given below:

2.1 Plant materials Lateral shoots (suckers) of A. indica (one month old) collected from experimental Aloe indica L. garden of BCSIR Laboratory, Rajshahi used as the explants. Explants were prepared by removing roots and brown colored tissues and extending leaf portions to give an average size of 3-4cm. They were washed thoroughly with running tap water for about 10 minutes till all soil and other foreign materials were washed off. Sets of twenty explants were then washed with tap water containing a few drops of Tween 20 and rinsed in 70% ethanol for 30 seconds followed by initial soaking in sodium hypochlorite containing approximately 4% available chlorine for 10 minutes and then in freshly prepared mercuric chloride solution (0.1 %) for 10 minutes. Finally, they were washed 3-4 times with sterile distilled water before culturing.

2.2 Culture media The basal medium used for the culture is MS medium [14] with 3% sucrose and 0.8% agar with growth hormones.

2.3 Shooting and culture conditions The pH of the medium was adjusted to 5.7 and then cultures were incubated at 25± 0⁰C, under cool white fluorescent tubes with 16 h photoperiod.

2.4 Shooting of Explants Explants devoid of contaminations were then inoculated on the MS basal medium supplemented with different concentrations of BA(0.5 mg/L), Kn (1.0mg/L) and BA (1.5 mg/L)) along in combination with NAA (2.0 mg/L). Shoots amplified from lateral shoot explants in shoot induction media were detached from explants and transferred onto a shoot elongation medium containing 1.0 gm/L activated charcoal.

2.5 Rooting of Micro shoots After growing of shoot (Two months old), 4-5cm tall shoots was inoculated into MS medium containing different concentrations of NAA, IBA and IAA individually. For NAA, IBA and IAA the concentration were 0.1 mg, 0.5 mg/L, 1.0 mg/L and 1.5 mg/L for each.

2.6 Hardening of plantlets About 5-6cm tall, well rooted plantlets were taken out from the culture vessels after one month from root induction media into plastic containers for acclimatization.

2.7 Statistical Analysis The experimental design was factorial with R.B.D basis design, which was done with unequal repetition. All the experiments were carried into ten replicates with four treatments. Data were subjected to ANOVA (analysis of variance) and significant differences between treatments were determined by DMRT using the SPSS software package.

Source of Information: IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372.Volume 5, Issue 1 (Sep.- Oct. 2013), PP 30-35

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Micropropagation is the process which used to save the endangered plant species as well as cost of various plantlet productions. In this thesis work, we found the encouraging result of hormone effects for commercial micropropagation of Aloe indica L. For future research more doses of hormone combination can be taken as treatments with fewer intervals which will give us specific result. At the same time except BA, Kn, NAA and IBA other types of cytokinin and auxine can be taken into consideration. Except shoot tip culture, meristem and callus culture could be practiced. The present study described an efficient protocol for shoot proliferation of Aloe vera with 90% development of shoots per explants with better quality plantlets in terms of growth. Higher shoot number reported in the present study in the shoot induction media could be attributed to the beneficial effect of activated charcoal in culture media. Rooting is more flexible in rooting medium. Moreover, 100% survival of plants after acclimatization could be achieved. Hence, the shoot regeneration procedure described in the present study could be ideal for rapid micropropagation of elite plants of Aloe indica L. By this protocol in future we could be decreased the cost of Aloe indica production which will lead the decrease of aloe product prices.

Knowledge Management: Journal