Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

A Comparative Study on Reproductive Hormones of Repeat Breeding and Synchronized Repeat Breeding Dairy Cows Under Bathan Rearing System at Baghabari Milk Shed Areas in Bangladesh

Royhan Ahammed
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Moumita Tasnim
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Md. Abdul Halim
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Marzan Sarkar
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Md. Sirajul Islam
Bangladesh Livestock Research Institute, Baghabari, Sirajgonj-6770, Bangladesh.

Mahbubul Morshed
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Abstract/ Executive Summary:

Repeat breeding (RB) is a major problem that causes a great economic loss in dairy herds. Farmers cannot get benefit from it as it increases production cost by insemination, treatment, feed, labor, calving interval, culling rates and decreased calf and milk production. A total of thirty (30) dairy cows of repeat breeder at the different sheds of dairy farmers at the Bathanrearing area of Shahjadpur Upazilla under Sirajgonj district in Bangladesh used for this study. The cows were synchronized in a standard heat period in which GnRH and PGF2α were treated. Blood samples were collected and separated serum samples were examined for the comparative study of the reproductive hormonal level of repeat breeder and synchronized repeat breeder dairy cows. The level of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone were significantly higher in synchronized repeat breeder cows than repeat breeder cows. The level of estradiol was also significantly higher in synchronized repeat breeder cows. The mean value of four reproductive hormones of normal repeat breeder cows were consequently (LH=0.0825mIU/mL, FSH=4.647mIU/ml progesterone =1.807 ng/mL,estradiol=82.23pg/mL) and in synchronized repeat breeder cows (LH=2.481mIU/mL, FSH=17.04mIU/mL, Progesterone=5.927 ng/mL, estradiol=107.3pg/mL).The results from the present findings clearly indicated that reproductive hormonal aberration might be one of the major causes of repeat breeding in dairy cows at Baghabari milk shed areas.

Keywords: Reproductive hormone, Synchronization, Artificial insemination, Repeat breeder cows.

Location: Different sheds of dairy farmers at the Bathanrearing area of Shahjadpur Upazilla under Sirajgonj district in Bangladesh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Hormone, Cow

Objectives:

Repeat breeding is one of the major problems affecting reproductive yield. The reason of repeat breeding is multifaceted; most common causes are genetical, anatomical defects of the reproductive regions hormonal imbalances, infections such as clinical, subclinical endometritis and poor management 22 . The objective of this study was to investigate the effectiveness of plasma hormones like FSH, LH, progesterone and estradiol in both repeat breeder cows and synchronized repeat breeder cows at Baghabari milk shed areas in Bangladesh to find out the conception aberration among the repeat breeder cows.

Methodology:

Study design: Prospective open-label observational study Study location: Baghabari milk shed areas of Sirajgonj district and in the Department of Biochemistry and Molecular Biology of Jahangir Nagar University, Savar, Dhaka, Bangladesh. Study duration: October 2017 to October 2018 Sample size: 30 repeat breeder cows with defects in pregnancy. Sample size calculation: The sample size was estimated on the basis of a single proportion design. The target population from which we randomly selected our sample was considered 30. Subjects & selection method: The study population was drawn from consecutive repeat breeder cows. These selected repeat breeder cows were marked by ear tag and collected the breeding history of each individual cow. Inclusion criteria: 1. Repeat breeder cows 2. Female 3. Mature aged 4. Symptomatically healthy Exclusion criteria: 1. Normal breeding cows 2. Feeble cows 3. Cows that are physically inactive.

Procedure methodology: A total of thirty (30) blood samples were collected into a vacutainer tube from the jugular vein of each experimental cow. Blood samples were collected before synchronization from all experimental cows. Poor rates of estrous detection combined with poor conception rates (CR) make management of reproduction in lactating dairy cows a challenge in most dairy herds. Blood samples were collected after synchronization measured by Estrous Detector Device for AI an appropriate time. The serum samples were prepared from all experimental cows. The serum samples were prepared in the animal Health Laboratory of Bangladesh Livestock Research Institute, Regional station, Baghabari, Shahjadpur, Sirajgonj in Bangladesh. The plasma serum was separated by centrifugation (1500 rpm for 20 min).The separated serum was collected in a sterile vial and preserved at −20°C Laboratory Deep freezer until analysis. All serum samples were analyzed in the laboratory at the department of Biochemistry and Molecular Biology, Faculty of biological Sciences, Jahangirnagar University (JU), Savar, Dhaka, Bangladesh by using endocrine detection kits (Mono LelacR USA).

Synchronization of experimental dairy cows: Experimental dairy cows were reared under the bathan feeding and management system. In addition, mineral supplements were given to all experimental cows. All experimental cows were synchronized by the treatment of GnRH (day-0), PGF2α (day-7) and Artificial insemination -AI + GnRH (day-9) by following the below estrous synchronization protocol for more efficient reproductive management of Repeat breeder cows. To help producer’s reproduction more efficiently, protocols for synchronization of estrus have been developed. The dairy cows that were identified as repeat breeder cows had been synchronized with the treatment of GnRH and PGF2α as described by Pursley, 199523.

Statistical analysis: Statistical analysis was performed with the two-tailed student’s t test. P values <0.05 were considered statistically significant. All statistical analysis was done using Graph Pad Prism 7 (La Jolla,CA)

Source of Information: IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372. Volume 11, Issue 11 Ver. II (November 2018), PP 55-60

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Repeat breeding is an important factor affecting economic success in dairy management. Whatever the cause of low conception, our result confirmed that synchronized repeat breeder cows had higher reproductive hormones as compared to repeat breeder cows. It is clearly demonstrated that reproductive hormones play a significant role in solving the repeat breeding problems of dairy cows. The use of these reproductive hormones may minimize the repeat breeding problems in dairy cows.

Knowledge Management: Journal