Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Genetic Differentiation of Wild and Hatchery Populations of Indian Major Carp Cirrhinus Cirrhosus in Bangladesh

Md. Rajib Sharker
Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Bangladesh

Muhammad Abu Bakar Siddik
Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Bangladesh

Ashfaqun Nahar
Department of Marine Fisheries and Oceanography, Patuakhali Science and Technology University, Bangladesh

Md. Shahjahan
Sado Marine Biological Station, Faculty of Science, Niigata University, Japan and Department of Fisheries Management, Bangladesh Agricultural University, Bangladesh

Abdullah Al Faroque
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Bangladesh

Abstract/ Executive Summary:

Genetic differentiation of Cirhinus cirrhosus, three river (Halda, Jamuna and Padma) and six hatcheries (Brahmaputra, Reliance, Kazi, Rupali, Bismillah and Pacific) populations covering a wide range of geographical distribution of this species were analyzed by allozyme electrophoresis. Four enzymes encoded by 7 loci were screened, where 3 loci were polymorphic (Mdh-1*, Mdh-2*, Gpi-1*). The results showed that polymorphic loci per population (28.57%), mean proportion of heterozygous loci per individual (10.000), mean number of alleles per locus (1.286), and relative gene diversity (0.148) in river populations were higher than those for hatchery populations (23.81, 6.677, 1.238, 0.140, respectively). Also, the observed heterozygosity (Ho) and expected heterozygosity (He) in river populations (0.086 and 0.112, respectively) were higher than those of hatchery populations (0.046 and 0.106, respectively). The pairwise population differentiation (F) values showed a lower level of genetic differentiation between hatchery and ST river population pairs. The unweighted pair-group method with arithmetic mean dendrogram of Nei's genetic distances showed a relationship between genetic distance and geographic distance. The population were clustered into three groups: Halda, Padma and Jamuna river population in one group; Brahmaputra, Reliance and Kazi hatchery populations in second group and Rupali, Bismillah and Pacific hatchery population in third group. The present study revealed that there were obvious genetic variations among the natural and hatchery populations.

Keywords: Allozyme electrophoresis, Allele frequency, Genetic diversity, Polymorphic loc

Location: In Bangladesh

Start Date:

End Date:

Program Area: Knowledge Management

Commodity: Carp fish

Objectives:

To assess genetic variation and relatedness in three river and six hatchery population of C. cirrhosus using allozyme markers.

Methodology:

Sample collection: The samples of Cirrhinus cirrhosus were collected from three main rivers (the Halda, the Jamuna and the Padma) and six hatcheries of three origins, (Brahmaputra and Reliance fish hatchery, Mymensingh; Rupali and Kazi fish hatchery, Jessore; Bismillah and Pacific fish hatchery, Comilla). Muscles were taken from each individual and o stored at –18 C until electrophoretic analysis. Allozyme electrophoresis: Horizontal starch gel electrophoresis and histochemical staining techniques were used to visualize different alleles according to Shaw and Prasad (1970). The enzymes including lactate dehydrogenase (LDH), malate dehydrogenase (MDH), phosphoglucomutase (PGM) and glucose phosphate isomerase (GPI) were analyzed for allozyme electrophoresis. Gel slices (1mm) were histochemically stained for different enzyme activities as outlined by Aebersold et al. (1987). Locus and allele designations were done following the standardized genetic nomenclature for protein-coding loci (Shaklee et al., 1990). Genetic data analysis: Allele frequencies were calculated simply by direct count of the proportion of different alleles. The distribution of observed genotypes were compared with the expected ones, calculated from Hardy-Weinberg (H-W) 2 equilibrium using Chi-square (χ ) test. Allele frequency and the 2 analyses of the Chi-square (χ) test were performed using Gene Alex (Peakall and Smouse, 2005) computer program (version 6.1). When the most common allele existed in frequency less than or equal to 0.95 at a given locus, the locus was regarded as a polymorphic. Mean proportions of polymorphic loci per population, mean number of alleles per loci and mean proportion of heterozygous loci per individuals, expected heterozygosity (He) and observed heterozygosity (Ho), Shannon's information index were analyzed with the help of POPGENE (Yeh et al., 1999) computer package program (version 1.31). Genetic differentiation (F) and gene flow (N ) were measured with the ST m help of Gene Alex (Peakall and Smouse, 2005) computer program (version 6.1). Based on Nei's genetic distance (D) (Nei, 1972), a dendrogram was constructed using the unweighted pair group method of arithmetic average (UPGMA).

Source of Information: Journal of Environmental Biology, Vol. 36, 1223-1227, September 2015; ISSN: 2394-0379 (Online)

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The electrophoretic patterns of C. cirrhosus indicated that enzymes were controlled by genes at seven presumptive loci. A maximum of two alleles (*a and*b) were found in Mdh-1* Mdh-2* and Gpi-1* loci with three genotypes (*aa, *ab, and*bb). On an average 1.857 genotypes were produced by 1.429 alleles at seven loci (Table 1). Among them, only two loci (Mdh-1* and Gpi1*) were found to be polymorphic in the Halda, Jamuna, Padma river and Brahmaputra hatchery population. Only Gpi-1* locus were polymorphic in the Reliance and the Kazi hatchery. The Rupali, Bismillah and Pacific hatchery population showed two polymorphic loci (Mdh-2*and Gpi-1*). The Jamuna river and the Brahmaputra hatchery population showed significant variation in allele frequency of Mdh-1* locus. In Mdh-2* locus allele frequency of the Bismillah, the Rupali and the Pacific hatchery populations showed significant variation. The Kazi hatchery and the Reliance hatchery populations showed significant variation in allele frequency of Gpi-1* locus. Genetic variabilities obtained in the present study indicated that hatchery populations maintained lower genetic variation, which might have serious genetic impact on the natural stocks if these inferior carp fry were being stocked in open waterbodies.

Knowledge Management: Journal