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Research Detail

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M T ISLAM
Plant Genetic Resources Centre, BARI, Joydebpur, Gazipur

R U CHOUDHURY
Plant Genetic Resources Centre, BARI, Joydebpur, Gazipur

An experiment was conducted with 50 germplasm of hyacinth bean (Lablab purpureus L. Sweet) to estimate the characterization and variability. Green and purple colours were found in hypocotyl, epicotyl and leaf veins among the germplasm. Absence of pigmentation, almost solid purple, extensive purple and purple localized to nodes were found on the main stem. The absence of leaf anthocyanin was observed among the germplasm. Pale to dark green leaf colour intensity was found. Variations were observed in ramification index, raceme position and orientation of pod bearing raceme. The germplasm was exhibited white (30%) and purple (70%) flowers. Green (60%), green with purple margin (26%), yellow-green with purple margin (2%), purple (8%) and white (4%) edible pod colours were found. Flat (56%), elongate (42%) and elongate wavy (2%) edible pod shape along with straight (68%) and slightly curved (32%) pod curvatures were observed. Variation of seed colour such as black (62%), brown (20%), yellow-white (6%), greyed-orange (6%) and bicolour-brown with cream (6%) was found. Small (28%), medium (36%) and large (36%) seed size along with ovoid (68%), flat (28%) and elongate (4%) seed shapes were exhibited. Days to first flowering were ranged from 48 to 92 days and edible pod stages were ranged from 63 to 126 days.  Variations were observed in leaf length and width, length of rachis and peduncle, number of flowering nodes per rachis and number of pods per rachis. A good range of variations was also observed in edible pod length (6.67 to 15.00 cm), width (1.78 to 4.50 cm) and pod weight (6.16 to 24.20 g). The germplasm was produced 63 to 710 pods per plant and 3 to 6 seeds per pod. The highest CV was observed in the number of pods per plant (49.38%) and the lowest was observed in leaf length (6.64%). Some promising germplasm were identified.

  Genetic variability, Hyacinth bean, Characterization
  Plant Genetic Resources Centre (PGRC) of BARI, Joydebpur, Gazipur,
  00-00-2015
  00-00-2016
  Variety and Species
  Hyacinth bean

The experiment was undertaken to study the genetic variability and to identify salient features that distinguish germplasm from one another

The experiment was conducted on hyacinth bean at Plant Genetic Resources Centre (PGRC) of BARI, Joydebpur, Gazipur, from October 2015 to May 2016. The location of the site is 24.000 N latitude and 90.260 E longitudes at an altitude of 8.40 m above sea level. Twenty-seven accessions and 23 newly collected germplasm were used in this study. One seed was planted in a plastic pot (6 cm in diameter approx.) containing a mixture of sandy-loamy soil and decomposed cowdung (1: 0.25) on 5 October 2015. Pits of 50x 50 x50 cm were prepared 10 days before transplanting the seedlings in the main field. The basal doses of fertilizers including cowdung were mixed well with the soil of the pits and the pits were prepared in such a way that the pit tips remained at least 10 cm above the ground level to facilitate drainage. The experiment was conducted in a randomized complete block design with three replications and each replication consisted of two plants. The unit plot size was 2 X 2 m. The seedlings were transplanted on 18 October 2015 in the pits. The plants were given the support of a 2 m height bamboo stick. Weeding was done whenever necessary. The plants were irrigated by plastic pipe whenever required. Regular spraying of wheel powder @ 2g/l was applied for controlling aphids but sometimes the insecticide ripcord @ 1.0 ml per litre was applied for controlling aphids and leaf borers. The recommended doses of manure and fertilizers such as 5 ton/ha cowdung, 60 kg N, 40 kg P, 60 kg K, 12 kg S, 3 kg B/ha, and 3.0.kg/ha Zn in the form of urea, triple superphosphate, murate of potash, gypsum, boric acid (solubor) and zinc sulphate (monohydrate) were applied in the experimental field (FRG, 2012). The full doses of cowdung, TSP, gypsum, solubor, zinc sulphate, and half doses of MP were applied during pit preparation before one week of transplanting The remaining MP and urea were applied in the three equal installments as top dressing at 15, 30 and 45 days after transplanting. Thirty-nine observations on qualitative (23) and quantitative (16) characters were recorded as per AVRDC-GRSU Characterization Record Sheet for Hyacinth bean (2000) and Descriptors for Vegetables and Condiments (1995). (Table 1 and Table 2). Range, mean, standard deviation, and coefficient of variation of quantitative characters were calculated

 

  Annual Research Report 2015-2016, Plant Genetic Resources Centre, BARI, Joydebpur, Gazipur
  
Funding Source:
1.   Budget:  
  

Green (59%) and purple (41%) colours were found in hypocotyl, epicotyl and leaf veins among the 50 germplasm of hyacinth bean. No pigmentation (50%), extensive pigmentation (40%), almost solid (8%) and localized to node (2%) were found on the main stem.  Pale green (20%), intermediate green (68%) and dark green (12%) leaf colour intensity were observed. One main stem with none or few short lateral branches were found in 70% germplasm,  followed by one main stem, few lateral branches starting from first nodes in 18% germplasm, and two or three main stems starting from first nodes were found in 12% germplasm. All the germplasm were exhibited the absent type of leaf anthocyanin, indeterminate climber and glabrous leaf pubescent. Raceme position was found within foliage (4%), intermediate (66%) and emerging from leaf canopy (30%). Upright (30%) and prostrate (70%) types of pod bearing raceme were found. White (30%) and purple (70%) flowers were found among the germplasm. Green (60%), the green pod containing purple margin (26%), purple (8%), white (4%) and yellow-green pod containing purple margin (2%) were found among the hyacinth bean germplasm. The distribution of edible pod shapes was flat (56%), elongate (42%) and elongate wavy (2%) while the curvatures of edible pods were straight (68%) and slightly curved (32%). Short (84%), long (2%) and thick beaks (14%) of edible pods were observed in the hyacinth bean germplasm. Five distinct seed colours such as black, brown, yellow-white, greyed-orange and brown with cream seeds (6 to 62%) were observed among the germplasm at the maturity stage after sun drying. The shape of dry seeds in the population was classified as ovoid (68%), flat (28%) and elongate (4%). Three seed sizes such as small, medium and large along with moderately ridged and markedly ridged were observed in the germplasm. Absent (70%) and brown spotted (30%) seed coat patterns were found.  All the germplasm produced flowers and seeds during winter and only 22% of germplasm produced flowers during April-May 2015. The qualitative descriptors for individual germplasm are presented in Table  3. 

Quantitative variations of 16 descriptors in hyacinth bean were observed. The highest leaf length of the terminal leaflet was exhibited in AHM-9 (12.5 cm) while the lowest 9.2 cm was observed in BD-10380. Terminal leaflet width was ranged from 8.5 cm (BD-10380) to 11.9 cm (AHM-9) with an average of 9.79 cm. The germplasm produced the first flower on 48 days (AH-34, K-4 and K-5) to 92 days (BD-10382) while the days to first edible pod stages appeared from 63 days (AH-32) to 126 days (AR-4-1). On average, days to first edible pod stage appeared at 25 days after the first flowering. Peduncle length and rachis length were ranged from 4.80 to 21.57 cm and 4.15 to 32.80 cm, respectively. Rachis length (21.15 cm) is about one and a half fold longer than peduncle length (15.82 cm). The germplasm produced 2.40 (RAI-51) to 11.45 (BD-2882) flowering nodes per rachis with an average of 8.31. The germplasm exhibited 4.0 (AHM-22/1, AHM-30) to 14.20 (BD-2916/1) pods per rachis in the population. The edible pod length was ranged from 6.67 to 15.00 cm in BD-10380 and AHM-22/11, respectively. On the contrary, pod width was ranged from 1.78 to 4.50 cm in AHM-22 and AHM-22/1, respectively. The germplasm produced pods weighing 6.16 to 24.20 g in BD-10380 and AHM-22/1, respectively. Such the lowest pod weight might be inherent characteristics of the germplasm.  The germplasm produced 63 to 710 pods per plant from AHM-22/1 and RAI-51, respectively while 3.80 to 6.00 seeds per pod were obtained from BD-2906 and RAI-51, respectively. An average of 274 and 4.6, pods per plant and seeds per pod were found, respectively from the germplasm. The variation in the number of pods per plant might be due to differences in the number of inflorescence per plant, pods per raceme, flower-dropping tendency and also the inherent potential of germplasm.  The germplasm exhibited 0.78 to 1.44 cm seed length from RAI-51 and AHM-22-1, respectively while 0.54 (AR-62) to 1.01 cm (BD-2888) seed width were obtained. The germplasm exhibited 26.02 g (RAI-51) to 67.52 g (AR-5) hundred seed weight with an average of 42.92 g. The maximum coefficient of variation was found in the number of pods per plant (49.38%) followed by edible pod weight  (28.74%), rachis length (28.02%), number of pods per rachis (26.07%),  peduncle length (25.81%),100-seed weight (25.27%), number of nodes per rachis (21.37%), edible pod length (18.13%), edible pod width (17.88%) and minimum cv was exhibited in leaflet length (cm) (6.64%). The quantitative descriptors for individual germplasm are presented in Table 4. On the basis of different qualitative and quantitative characters, the germplasm BD-2881, BD-2896, BD-2918, AHM-22-1 and AHM-110 may be selected as an elongate pod (12 to 15 cm); BD-2918/1, AHM-22/1, AHM-110, AR-4/1 and K-1 as a flat pod (3.0 to 4.5 cm);  BD-2896, BD-2918, AHM-22/1, AHM-30, AHM-110 and AR-5 as maximum pod weight (12.42 to 24.20 g); BD-2882, RAI-51, IA-20, IA-21, AR-4-2 and AR-73 as the maximum number of fruits (413 to 710); AH-32, AH-34, AR-62, K-4 and K-5  as early flowering (48 to 52 days) and AH-32, AH-34, AHM-110, K-1 and K-5 as early fruiting (63 to 69 days); BD-2882, BD-2901, BD-2916/1, BD-10382 and AR-4/1  as late flowering (80 to 92 days), BD-2878, BD-2888, BD-2896, BD-2914, AR-4 and AR-5 as bold seeded (54.6 to 67.5 g per hundred seed) germplasm. The sufficient seeds are kept in conservation for future use.

 

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