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Comparative Study on Proximate Composition and Amino Acids of Probiotics Treated and Nontreated Cage Reared Monosex Tilapia Oreochromis Niloticus in Dekar Haor, Sunamganj District, Bangladesh

M Biswas
Mithila Biswas, Department of Coastal and Marine Fisheries, Sylhet Agricultural University, Sylhet, Bangladesh

MS Islam
Mithila Biswas, Department of Coastal and Marine Fisheries, Sylhet Agricultural University, Sylhet, Bangladesh

Abstract/ Executive Summary:

The study was carried out to identify the impact of probiotics on proximate composition and amino acids of cage reared tilapia Oreochromis niloticus for 120 days. Cage farming was done under four treatments as T1 (biozyme), T2 (rapid grow), T3 (miracure probiotics) and T4 (control), and each having three replicates. Proximate compositions and amino acids of fish were determined. Proximate composition and amino acid profiles varied significantly (p<0.05) between probiotic treated and nontreated fish. Higher amount of moisture and ash content observed in nontreated tilapia whereas comparatively higher protein and lipid content found in treated than nontreated. Among 14 recorded amino acids, essential amino acids were threonine, methionine, isoleucine, leucine, histidine, lysine, arginine and valine. Nonessential amino acids were aspertic, serine, glutamic, glycine, alanine and tyrosine. Comparatively higher amount of essential and nonessential acids secured in treated tilapia than nontreated tilapia. Therefore, results of the study imply that probiotics may use in tilapia farming to enhance protein and lipid content, which help to increase taste and good smell of fishes.

Keywords: Proximate composition, Amino acids profile, Probiotic, Monosex tilapia

Location: Dekar haor, Sunamganj district, Bangladesh

Start Date: 01-09-2016

End Date: 29-12-2016

Program Area: Animal Health and Management

Commodity: Tilapia

Objectives:

This study can provide systematic information regarding the nutritional composition of muscles of fish, which will be fundamental information for the consumers, producers and researchers. Considering the above facts, the present research was undertaken with aims to investigate proximate composition and amino acid profiles of probiotics treated and nontreated cage reared tilapia (O. niloticus) in haor conditions.

Methodology:

2.1 Study area and experimental period Tilapia were collected from an tilapia cage farming in Dekar haor (naturally depressed seasonal-perennial open waterbody), where the fishes were fed probiotics mixed commercials feed and only commercial feed. The haor is consisted of many small, medium and large interconnecting beels, canals, rivers and crop lands. The experiment was carried out for a period of 120 days from 1 September to 29 December 2016 in 12 floating cages set up at the periphery of the haor.

2.2 Collection and preparation of sample As a sample 12 fishes were collected from each treatment (T1-biozyme, T2-rapid grow, T3-miracure and T4-control) of tilapia cage culture until harvest. Monthly intervals samples were taken from the floating cages of tilapia. Only fresh muscle tissue from dorsal region without skin and bone of the fish body were collected and chopped then grinded by mortar and pestle to make a homogenous sample. The determination of proximate composition as well as amino acids profiles were done in laboratories.

2.3 Analytical procedures of proximate composition Proximate composition as moisture, ash, crude protein and lipid of fish muscles was determined by conventional method of AOAC with minor modification. For determination of moisture content 15 g fish samples were taken into a hot air oven at a temperature of 105⁰C for 24 h. until constant weight was obtained. A total of 5 g samples were taken into a muffle furnace at a temperature of 600⁰C for 6 h. for ash content measurement. Kjeldahl method was used to determine crude protein content of fish samples. For most routine purposes, the percent of protein in the sample was then reckoned by multiplying the percent of nitrogen with protein conversion factor of 6.25 for fish. A ground joint soxhlet apparatus was used for the determination of lipid content.

2.4 Determination of amino acid profiles The profile of amino acids was done following high-performance thin layer chromatographic (HPTLC) method [3]. The HPTLC was analyzed the profile of amino acids in the muscle of tilapia.

2.5 Statistical Analysis: Statistical analyses were performed using one way analysis of variance (ANOVA) and Duncan’s multiple Range Test was used to determine differences between treatments means at significance rate of p<0.05. The standard deviation of treatment means was also estimated. All statistics were carried out using Statistical Analysis program (SPSS, 20).

Source of Information: International Journal of Fisheries and Aquatic Studies 2018; 6(2): 431-435

Article Link (Online Journal):

Funding Source:

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Achievement/Findings:

Tilapia (O. niloticus) has high cultural value and consumers prefer it all over the country. This is an excellent candidate for monoculture or mixed/polyculture with other species in seasonal waterbodies owing to it is hardy, diseases resistant and it may live in poorly oxygenated water. It is commercially important due to its high demand for protein. Findings of the study conclude that there is much variation in proximate composition and amino acid profiles between probiotics treated and nontreated cage cultured tilapia. Amount of moisture and ash is higher in nontreated tilapia compared to treated tilapia. On the contrary, treated tilapia contains higher amount of protein, as well as lipid owing to treated tilapia, are fed with probiotics supplementd feed, which is more beneficial for fish and other animals. As a result probiotics treated tilapia is an excellent source of protein and lipid because higher amount of amino acids composition and degree of digestibility. So it is more preferable and tasty fish than nontreated fish.

Knowledge Management: Journal