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Induced Spawning and Larval Rearing of Endangered Species Nandus Nandus (hamilton) in Cemented Cistern Using Pituitary Hormone Injection

Mst S Sharmin
Bangladesh Fisheries Research Institute (BFRI), Bangladesh

Md Golam Sarwer
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh

Abstract/ Executive Summary:

The present study deals with brood development at different stocking densities, Standardization of induced breeding technique and larval rearing of Nandus nandus. To develop N. nandus broods, average body weight (Male 20.50 ± 3.7cm, Female 14.50 ± 4.0 cm) were reared 3 months at different densities (T1= 40/deci T2= 60/deci T3= 80/deci). Feeding with fry, dead small fises and artificial feed contain 30% rice bran, 30% wheat bran, 20% fish meal, 15% MOC, 5% molasses two times daily@ 5-3% body weight was done. Brood was highest wt. (Male 34 ± 4.20gm Female 62 ± 5.10 gm) in case of density T1= 40/deci. For standardization of breeding technique of N. nandus three different doses of PG were tested viz 4, 6, 8 mg/kg BW for female and 1.5, 2, 2.5 mg/kg BW for male. Sex ratio male: female =1:1 was maintained. PG treatment with 1.5mg/kg for male and 4 mg/kg for female revealed best fertilization (95 ± 5) % and hatching (88 ± 2) %. After 72 hours hatchlings were transferred to pond for larval rearing for 20 days. Average Length attained from (4.01 ± 0.05) to (23.02 ± 0.245) mm and weight (0.90 ± 0.003) to (370.00 ± 0.025) mg for 20 days rearing.

Keywords: Nandus nandus, Brood development, PG, Induced breeding, Larval rearing

Location: The floodplain sub-station, Bangladesh Fisheries Institute, Santahar, Bogra.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Fish seeds, Hormone

Objectives:

Therefore, the fish is going to be rarely available and now considered to be an endangered or threatened species. International union for Conservation of nature has enlisted mottled nandus (N. nandus) in their red list of threatened fishes of Bangladesh as a vulnerable taxon. With a view to overcome the odd situations induce breeding and development of culture techniques of N. nandus became important which will ensure its conservation and rehabilitation through increasing its production. That’s why the present study has been undertaken to standardize of artificial propagation of this species using pituitary hormone injection.

Methodology:

Study area: The experiment was conducted during June to August, 2015 in the cemented cistern having size 2.74×1.5×0.5 m of the floodplain sub-station, Bangladesh Fisheries Institute, Santahar, Bogra. Brood fish collection Required number of healthy male and female N. nandus broods were collected from the Raktodahabeel in Sntahar, Bogra and stocked in the previously prepared brood rearing ponds. Brood rearing The collected broods were reared for two months in the brood rearing ponds at a stocking density of 40-45ecimal. Pond size was 10 decimal and water depth 1.0 meter. During rearing lime, fertilizer, and cow dung were properly applied. Fertilization with Urea and TSP at the rate of 150g/decimal and 75g/decimal respectively were applied at 15 days interval. Broods were fed with artificial feed (contain 30% ricebran, 30% wheat bran, 20% fish meal, 15% MOC, 4% molasses and 1% vitamin premix) two times daily at the rate of 5% body. Water quality parameter were recorded fortnightly during rearing period. Brood selection For successful induced breeding only healthy, uninjured and sexually mature broods were selected. Mature male and female broods were identified on the basis of secondary sexual characteristics. Maturity of fish was determined by following ways.

Conditioning of broods Selected brood fish were kept in cemented cistern for about 6 hours for conditioning prior to injection with PG extract. Handling and carrying of fish was done very carefully to avoid possible injury and secondary infection. Males and females fish were kept in separate cistern and constant water flow was maintained to ensure proper aeration. Preparation of PG solution PG were collected from market in preserved condition in airtight vials and used as inducing agent. At first, PG were gently removed from the vial with a pair of forceps and then weighed by an analytical electronic balance. The amount to be weighed out was calculated on the total of the body weight of all the fishes using the following formula: Weight of PG (mg) = (Wt * Pt) /1000 Where, Wt represents total body weight (g) of all the fishes to be injected and Pt represent the rate in mg PG to be injected/kg body weight under a particular treatment. PG was diluted in distilled water to dissolve it and centrifuged with a hand centrifuge for precipitation. The freshly prepared supernatant solution of hormone was then taken slowly in a 1 ml. hypodermic syringe for injection. PG injection in to brood fishes One ml disposable syringe was used for injecting hormone to the recipient fish. The appropriate amount of diluted hormone stock solution was taken in the syringe. Then the fishes were caught very carefully from the spawning cistern. A piece of clean soft and wet cloth was used to wrap up the fish and kept lying on a table. The accurate dose of PG extract was administered at the basal part of the pectoral fin. Needle was inserted at an angle of 450 with the body. For standardization of induce breeding of Nandus nandus three different doses of PG were tested.

Spawning behavior After hormone injection both male and female breeders were kept (male: female =1:1) in fine soft cloth made hapa in cemented cisterns and artificial shower of water was provided. After 6-8 hours of hormonal injection, fish started spawning activities. Male actively moved around the female started vent kissing. A male was observed to creep up to a female and to position himself to her near. Male bent its body with female and tried bring genital pore together. Play continuous for hours together finally female ejects brown colored egg and male ejected milt on the released egg. After releasing of eggs brood fishes were removed from the hapa. Fertilization and hatching The eggs released by the female were allowed to fertilize automatically by the milt of the male in the spawning cistern. Fertilization were also occurs with splashing movement. A total of 100 eggs from each hapa were transferred into a bucket and classified as fertilized or unfertilized under binocular microscope. The fertilization rate was calculated as the number of fertilized eggs divided by the total sampled number (n=100) of eggs. After 18-20 hrs of injection all fertilized eggs were hatched out. Unfertilized eggs and egg mollusca eggs were cleaned out of the cistern within an hour of hatching to protect larvae from fungal injection. Hatchlings were fed with boiled chicken egg yolk after 36 hours. The rate of hatching was estimated from the number of hatchling to the number of fertilized eggs, multiplied by 100.

Source of Information: International Journal of Fisheries and Aquatic Studies 2016; 4(5): 109-113

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present experiment was provided Standardization of Induced breeding and larval rearing of endangered Species Meni (Nandus nandus). This experiment will help to the endangered species (Nandus nandus) escape from extinction and thereby ensuring biodiversity conservation in nature. As this species is highly demandable further research is needed to start commercial seed production and culture of this species.

Knowledge Management: Journal