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Extraction and Identification of Pufa from African Catfish (clarias Gariepinus) Skin

Abu Sayem Md. Ahsan Habib
Scientific officer
Dr. Wazed Research Institute Begum Rokeya University, Rangpur, Bangladesh.

Dr. Prosannajid Sarkar
Senior Scientific officer
Dr. Wazed Research Institute Begum Rokeya University, Rangpur, Bangladesh.

Abstract/ Executive Summary:

Fish and fish oil is the major source of polyunsaturated fatty acids (PUFA). These PUFA are variously important for human health. In this experiment, the most cheap and fatty fish species was used for the extraction and identification of PUFA using soxhlet extraction method and gas chromatography (GC) analysis revealed that 0.80%, 2.48% EPA and DHA were present in the test sample, respectively. The level of different fatty acid content range between 0.03% to 22.6%. Therefore, the objective of this study was to extraction and identification of PUFA from the cheaply available catfish waste part (skin) for the rural poor Bangladeshi.

Keywords: Fish oil, Polyunsaturated fatty acid (PUFA), EPA, DHA

Location: Centre of Excellence for Food Safety Research (CEFSR) Laboratory, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

Start Date: 00-06-2010

End Date: 00-08-2010

Program Area: Resource Development and Management

Commodity: Cat fish

Objectives:

The waste materials from the African Catfish, mainly the skin, can be a reliable source of raw material for industrial scale the extraction of lipid at as it has minimal/negligible seasonal variation regarding chemical composition throughout the year (Osibona et al., 2009). Therefore, the objective of this study was to extraction and identification of PUFA from cheaply available catfish for the rural poor Bangladeshi.

Methodology:

Experimental site The experiment was conducted on June 2010 to August 2010 in Centre of Excellence for Food Safety Research (CEFSR) Laboratory, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. Materials Fresh African Catfish (Clarias gariepinus) samples were collected from the local market of Malaysia. All chemicals and solvents used in this experiment were analytical grade and obtained in Malaysia. Sample Preparation for Experiments The fresh samples were immediately de-headed and washed with copious amounts of fresh cool water to separate the skin. Then the skin were separated by using a de-boner and immediately the experimental sample were stored overnight in a freezer at −18 °C, and then freeze dried (Model: Labconco, USA) at a constant drying temperature of −47 °C and vacuumed at 0.133 bar. The dried samples were ground using a blender and stored in an airtight glass bottle in a cold room at 6 °C pending laboratory use.

Moisture Content Determination The moisture content of the dried sample was determined by the oven dry method (AOAC, 1994). Five grams of finely ground sample was place in pre-weighted ceramic crucibles before being put into the oven. The temperature of the oven was set at 105 °C, and the heating process continued until constant weights of the samples were achieved. Then, the crucibles were transferred to a desecrator to cool before reweighing. The difference between the two weights (initial and final) indicated the moisture content (3.10 ± 0.1%) of skin. Soxhlet Extraction Total lipid content from the catfish skin was determined by soxhlet extraction method. Five grams of finely ground dried samples were extracted with 200 mL of petroleum ether with three replications over an extraction period of 8 h. Extra water and petroleum ether residue in the extracted oils were evaporated using a rotary evaporator (Heidolph, Germany) at a temperature of 45 °C. The evaporated sample was then kept in an oven at 45 °C for 1 h for the confirmation to totally disappear moisture and petroleum ether. Total lipid content of the catfish skin was 48 ± 0.4%/100g sample based on the dry weight basis.

Source of Information: International Journal of Fisheries and Aquatic Studies 2016; 4(4): 312-314

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the findings of this study, I would like to suggest the poor rural Bangladeshi to consume C. gariepinus fish species with their skin very cheaply to avoid excessive and excessive consumption of saturated fat rich poultry and beef protein. Moreover, due to having EPA and DHA could be a good health promoter. Therefore, the studied fresh water fish species found to be important sources of nutrients.

Knowledge Management: Journal