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Growth Regulatory Activities of Aqueous Extracts of Five Ornamental Plants

Sona R. Roy
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

B. Roy
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

Bikash C. Sarker
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

M. Nazim Uddin
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

M.S. Zoha
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.

Abstract/ Executive Summary:

A study was conducted on activities of aqueous extracts of leaves of five ornamental plant species on four summer vegetable crops. The aqueous extract of Butea monosperma (parrot tree) leaves markedly enhanced the germination, shoot and root length of all the four vegetable crops. The growth of shoot and root length of these vegetable crop species were enhanced using aqueous leaves extract of B. monosperma indicating the presence of growth promoting substance(s). The thin layer chromatography (TLC) examination of chloroform crude extract of B. monosperma showed three different compounds at hexane: ethyl acetate (5:1 v/v).

Keywords: Chemical investigation, Germination, Growth regulatory activity, TLC

Location: Laboratory of Agricultural Chemistry and Biochemistry in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

Start Date: 00-07-2006

End Date: 00-07-2007

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants, Growth regulator

Objectives:

Some positive growth regulators (terpenoids, olefinic and ketone compounds) have been isolated from Dillenia indica with the help of isolation technique (Shil, 2007). The present study was undertaken to examine whether they have growth regulatory effect of some ornamental plants leaves available in Bangladesh on germination of summer vegetables crop seeds and their primary growth rate or not, and also to isolate and identify the ingredient of growth regulatory substance from aqueous extracts.

Methodology:

The experiment was conducted at the Laboratory of Agricultural Chemistry and Biochemistry in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh, during July 2006 to June 2007. The aqueous extracts were prepared from some selected ornamental plants, i.e., Butea monosperma, Artabotrys odoratissimus, Tabernaemontana coronara, Ixora coecinia and Mussaenda erythrephytta leaves. The freshly prepared aqueous extracts were applied on seeds of country bean (Lablab niger), lady’s finger (Hibiscus esculentus), swamp cabbage (Impoea aquatica) and yardlong bean (Vigna unguiculata) to observe their germination and subsequent growth performances of seedlings.

Two sheets of filter paper (Whatmann No. 1) were placed on a Petri-dish (10 cm inner diameter). Total 72 Petri-dishes were set for 6 treatments with 4 tested vegetable crop species, while tap water was used for control. Each treatment was replicated for three times. Twenty five seeds of each vegetable crop were placed on each Petri-dish moistened with 15 ml aqueous extract of ornamental plant leaves and were kept at 27±2 0C temperature and 85±2% relative humidity. The filter paper is kept constantly moist with water. The experiment was laid out by completely randomized design while tap water was used for control. Each treatment was replicated for three times. The germination percentage, shoot and root length of seedlings were measured regularly after seed germinating in the Petri-dish. The measurements were taken at seven days after germination. The data were analyzed statistically and the differences between means were compared by LSD followed by DMRT.

The 100 g of leaves powder (previously sun dried) was taken in a 2.5 L bottle containing 500 ml chloroform and kept for 72 h with regular interval of shaking. It was then filtered by using Whatmann filter paper no. 1. The filtrate (450 ml) was collected in a 500 ml bottle and 200 ml chloroform was also poured again to the residue. This process was continued for three times. These collected filtrates were mixed together and was evaporated by using thin film rotary evaporator under reduced pressure. After collecting crude from rotary evaporator, it was used for TLC preparation. TLC was done to detect the presence of compounds in the crude extract.

Source of Information: J. Sci. Technol. (Dinajpur) Vol. 6:121-125 (2008) ISSN 1994-0386

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From these findings, it is very difficult to draw a definite conclusion but initial results suggest that aqueous extract of leaves of B. monosperma enhances the germination and growth of a number of some summer vegetables.

Knowledge Management: Journal