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Research Detail

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S. Saha
Student
Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh

M. Ahmed*
Professor
Agrotechnology Discipline, Khulna University, Bangladesh

M. M. Islam*
Professor
Agrotechnology Discipline, Khulna University, Bangladesh

R. N. Remme
Lecturer
Agrotechnology Discipline, Khulna University, Bangladesh

M. R. Ali
Professor
Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh

The present research work was undertaken to choose the most effective sugar concentration in in-vitro microtuberization from cultured shoot tips and to select the best substrates for minituber production from ex vitro transferred plantlets. Percentage of survivability of microtuber producing plants increased with the increase of sucrose concentration. Best survival rate and average diameter of tuber per plant was found at 10% sucrose. Minituber production on direct field showed best performance. It was observed that after 30 days, maximum shoot length per plant (188.87 mm) was found on the direct field whereas the lowest shoot length per plant (66.81 mm) was on coconut dust. Lowest weight of tuber per plant was also recorded on the substrates containing coconut dust. The highest weight of tuber was found in direct field. In case of, average diameter of tuber per plant, 25% soil + 75% coconut dust showed minimum result and on the other hand, in direct field, large size of tubers were found.

  Microtuberization, Minituber, In vitro, Ex vitro, Potato
  Plant Tissue Culture Laboratory of Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh
  00-04-2010
  00-01-2011
  Resource Development and Management
  Potato, Nutrition

In in vivo condition average tuber weight and tuber number were determined as different depending on the production technique (Struik, 1999). Khuri et al., (1995) found that the tuber numbers of various potato cultivars were between 6.2 to 7.9 in plantlets grown in polyethylene bags. Present research work has been undertaken to fulfill the following specific objectives to choose the most effective sugar concentration in in vitro microtuberization from cultured shoot tips and to select the best substrates for minituber production from ex vitro transferred plantlets.

2.1 Experimental site: The experiment was carried out at the Plant Tissue Culture Laboratory of Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh, during April 2010 to January 2011.

2.2 Type of explants: In vitro grown potato explants were used as source materials for microtuberization and minituber production. 2.3 Constituents of media for microtuberization of potato: For microtuber formation, MS medium supplemented with BAP-5.0 mg l-1 , Chloro Choline Chloride (CCC)-500 mg l-1 and different concentrations of sucrose-5%, 6%, 7%, 8%, 9%, 10% is used. 

2.4 Substrates used in pots for minituber formation: Following substrates were employed for minituber production: 1. Soil 100% 2. Sand 100% 3. Coconut dust 100% 4. Soil : Sand 50% : 50% 5. Soil : Coconut dust 25% : 75% 6. Soil : Coconut dust 50% : 50% 7. Soil : Coconut dust 75% :25% 8. Directly into the field

2.5 Experimental design All the experiments were laid out in a Completely Randomized Design (CRD) with five replications for each treatment. In that case, a plastic pot containing different substrates with a single healthy plant was considered as a replication.

2.6 Procedure for microtuberization After taking all the precautions to ensure aseptic condition, in vitro grown healthy plantlets were selected for further inoculation. All inoculation and aseptic manipulations were carried out under a laminar airflow cabinet. planlets were removed carefully and cut the shoot tips measuring 2 cm in length. Then they were placed onto nutrient media containing test tubes for microtuberization. After inoculation of the explants, the microtuber induction cultures were incubated in the dark at 24°C and relative humidity 65%, maintaining a temperature of 18o C. Microtuber was first observed at 35 days after inoculation. The experiment was laid out in a Completely Randomized Design (CRD) with ten replications. A test tube (15 cm length) containing 15 ml medium with a single shoot tip of potato was considered as a replication. 

2.7 Procedure for minituber production For minituber production of potato, in vitro cultured plants were transplanted into plastic pots (4 inch size) with different substrates (soil, sand, coconut dust and their different combination) and some were directly into the field. Soil, sand and coconut dust were autoclaved at 121o C for 15 minutes before transplantation. After proper mixing of different substrates according to each treatment, they were kept in plastic pots. Few minutes later after watering, plants were transferred into pots. Transferred plants were placed into growth chamber with 85% humidity for 24 hours. After that, they were kept in net house.

2.8 Analysis of data Recorded data for all of the experiments were analyzed for ANOVA (Analysis of Variance) with the help of computer using MSTAT-C program and the means were compared according to Duncan’s Multiple Range Test (DMRT) and Least significance Differences (LSD).

  IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372.Volume 4, Issue 6 (Sep. - Oct. 2013), PP 58-62 www.iosrjournals.org
  
Funding Source:
1.   Budget:  
  

In in vivo condition average tuber weight and tuber number were determined as different depending on the production technique (Leifert and Struik, 1992; 1999). Keller et al., (2006) found that the tuber numbers of various potato cultivars were between 6.2 to 7.9 in plantlets grown in polyethylene bags. In the study of minituber production, result showed that plants which were directly transplanted into field represented best result than other substrates.

  Journal
  


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