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Research Detail

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D. Das
Assistant Professor
Agrotechnology Discipline, Khulna University, Khulna

I. Kabir
Senior Officer
Agrani Bank Ltd, Dhaka

M. Islam
Professor
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

The experiment was conducted in the field laboratory of the Department of Crop Botany, Bangladesh Agricultural University (BAU), Mymensingh during the period of November, 2009 to September, 2010 to investigate the effect of spray formulations of NPK fertilizer on morphophysiological characters and growth of two orchid cultivars. The two factor experiment was laid out in the Completely Randomized Block Design (RCBD) with three replications. The two factors are cultivars (Mokara Diheard Red and Mokara Yellow Anne) and four spray formulations (F0, F1, F2, F3). Data were collected on plant height, leaf length, leaf width, total leaf area, leaf area index, stem diameter, number of leaf, chlorophyll content, crop growth rate (CGR) and relative growth rate (RGR). Results revealed that the morphological and growth parameters of Mokara Diheard Red and Mokara Yellow Anne were significantly varied. Mokara Diheard Red performs better in chlorophyll content, CGR and RGR. Mokara Yellow Anne was superior in respect of leaf area index, total leaf area, leaf number and stem diameter. It was observed that the maximum increment of total leaf area, leaf area index, leaf number and stem diameter were found in spray formulation-3 (F3) and the lowest were in control (F0). Spray formulation-2 (F2) showed maximum increment of plant height, crop growth rate and relative growth rate over control (F0). So, the morpho-physiological features of orchid are significantly affected by spray formulation and the composition of spray formulation-2 (F2) and spray formulation-3 (F3) might be better for growth of orchid. 

  Orchid, Spray formulation, Mokara Diheard Red, Mokara, Yellow Anne, Growth
  Department of Crop Botany, Bangladesh Agricultural University (BAU), Mymensingh
  00-11-2009
  00-09-2010
  Pest Management
  Orchid

The schedule of application of spray formulation recommended by Bangladesh Agricultural Research Institute (BARI) is too much variable in every week that is very difficult to maintain. This problem can be overcome by rescheduling the time of application of spray formulation. So, an experiment was undertaken to determine the effect of spray formulation on morphophysiological feature and growth of two orchid cultivars as well as to select suitable concentration of N, P and K in spray formulation for proper growth of orchid. 

Experimental Material Two cultivars of orchid named Mokara Dihard Red and Mokara Yellow Anne were collected from Dipto orchid nursery, Valuka, Mymensingh. 

Experimental Site: The experiment was conducted in field laboratory of Crop Botany Department, Bangladesh Agriculture University, Mymensingh. Experimental Treatments There are two factors used in this experiment known as variety and formulation of spray nutrients. Factor A: Two orchid cultivars viz. Mokara Diehard Red (V1) and Mokara Yellow Anne (V2) Factor B: Three levels of spray nutrient with control (F0) viz, Spray Formulation 1 (F1), Spray Formulation 2 (F2), Spray Formulation 3 (F3) Thus, the total number of treatments was 8 (4x2) and each treatment was replicated three times. The spray solutions were prepared by mixing of Urea, Tripple Super Phosphate (TSP) and Muriate of Potash (MoP). Formulation was prepared by following the thumb rule of BARI where total amount of fertilizer to be 700 g per 172 litre water was recommended for use as spray. Spray formulation used in early stages contained higher N and gradually reduced in later stages. % of N, P and K in Urea, TSP and MoP is 46.0, 48.0 and 60.0 respectively. In each times fresh formulation was prepared and sprayed once weekly with a hand sprayer in the afternoon. 

Collection of Data Data were collected periodically on Plant height (cm), leaf length (cm), leaf width (cm), individual leaf area (cm2 ), total leaf area (cm2), leaf area Index (LAI), stem diameter (cm), number of root/plant, number of leaf/ plant, chlorophyll content in leaf, fresh weight (g) of plant sample and dry weight (g) of plant sample. Experimental Design and Statistical Analysis The experiment was laid out in Completely Randomized Block Design (RCBD) with 8 treatments and each treatment was replicated three times. The collected data analyzed by following the analysis of variance (ANOVA) technique and mean differences were separated by Duncan’s Multiple Range Test (DMRT) (Gomez and Gomez, 1984) using a computer-operated programme named MSTAT-C (Russel, 1986). 

 

  IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372.Volume 5, Issue 5 (Nov. - Dec. 2013), PP 54-60 www.iosrjournals.org
  
Funding Source:
1.   Budget:  
  

Based on experimental results it may be concluded that i) The morphological and physiological attributes was significantly affected by spray formulation. ii) Spray formulation-2 (F2) was the best for growth of orchid. iii) The ratio of N, P and K at 10:15: 20, 15:20:25, 10:25:25, and 10:25:30 for spray formulation might be the best for growth of orchid.

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