Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Prevalence and Antibiotic Resistance Pattern of Escherichia Coli Isolated from Raw Dairy Milk

Abul Hasan
Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal, Bangladesh.

Md. Ahsanur Reza
Department of Physiology and Pharmacology, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal, Bangladesh.

Farzana Islam Rume
Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal, Bangladesh.

Mirza Mienur Meher
Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal, Bangladesh.

Md. Shafiullah Parvej
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.

AKM Mostafa Anower
Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal, Bangladesh.

Abstract/ Executive Summary:

E. coli is one of the most important food-borne pathogens, which could be transmitted by milk and milk products. To assess the role of dairy milk as the source of drug-resistant E. coli, we examined 50 raw dairy milk samples (25-farm milk + 25-market milk) from some selected areas of Bangladesh by cultural, morphological, biochemical and antimicrobial sensitivity tests. In the preliminary observation, the mean total aerobic mesophilic count of the market and farm raw milk samples were 8.98 and 8.68 log CFU/ml, while the mean coliform count were 4.20 and 3.03 log CFU/ml respectively. Thirty-three E. coli isolates were recovered from collected samples (66% 33 of 50) and this pathogen was more prevalent in market milk (76%, 19 of 25) than farm milk (56%, 14 of 25). In addition, most of the isolated E. coli exhibited resistance against ampicillin and cefotaxime. This result shows that, raw dairy milk and its products could be a source of human drug-resistant E. coli.

Keywords: Dairy milk, E. coli, Total aerobic mesophilic count, Coliform count, Drug resistance

Location: Jessore district in the southwestern climatic zone of Bangladesh

Start Date:

End Date:

Program Area: Quality and Nutrition

Commodity: Milk, Bacteria

Objectives:

To assess the role of dairy milk as the source of drug-resistant E. coli.

Methodology:

Sample Collection We collected 25 dairy raw milk samples from five different villages and 25 samples from respective villages linked five local markets located in Jessore district in the southwestern climatic zone of Bangladesh. We used sterile container for sample collection and transportation and each sample contained 150 ml of fresh raw milk. In addition, we made a survey among 50 participants in the study area by a cross-sectional questionnaire including their educational qualification, personal sanitary condition, source of drinking water, consumption of raw milk and milk products to elucidate the awareness of the peoples in that zone of the country. Determination of Total Aerobic Mesophilic Count and Coliform Count To determine the total viable count of milk samples, 0.1 ml of each ten-fold dilution was transferred and spread on plate count agar (PCA) using a sterile glass spreader (Erkmen, 2015). The plates were then incubated at 37°C for 24 hours. Following incubation, plates exhibiting 30- 300 colonies were selected to count. The average number of colonies in a particular dilution was multiplied by the dilution factor to obtain the TAMC according to ISO (1995). The results were expressed as colony-forming units per ml (CFU/ml) of milk sample. The procedures for coliform count (CC) were similar to those followed in TAMC except MacConkey agar was used. Isolation of E. coli A quantity of 0.1 ml inoculums from dilutions was used by spread plate technique on Eosin Methylene Blue agar (EMB) (Himedia, India). Incubated at 37°C for 24 hours. Typical characteristics colony of E. coli on EMB agar as greenish metallic sheen was enumerated and isolated by subculturing into EMB. Isolated organisms with supporting growth characteristics of E. coli were subjected to Gram’s staining, sugar (dextrose, fructose, maltose, lactose and sucrose) fermentation, methyl red-voges Proskauer test (MR-VP) and indole production test following the procedure mentioned by (Erkmen, 2015). Antibiotic Sensitivity Test Antibiotic sensitivity test of isolated E. coli was performed with standardized commercial antibiotic discs (Oxoid, UK) following Disc Diffusion Method (Bauer et al., 1966). Sensitivity to antibiotic was studied on Muller Hinton agar plates (Himedia, India) with ampicillin (10μg/disc), chloramphenicol (30μg/disc), ciprofloxacin (5μg/disc), gentamicin (10μg/disc), cephalexin (30μg/disc). An amount of 0.1 mL freshly grown pure culture of E. coli in nutrient broth (turbidity was compared with 0.5 McFarland standards) was poured on agar plates and allowed to spread gently over the entire surface with a glass rod spreader. After 5 minutes, the discs were placed at a distance of about 1 cm apart and incubated at 37°C for overnight. Based on the diameter of zones of inhibition produced around the antibiotic discs the inhibitory effect of the antibiotic to the growth of the culture was recorded and analyzed according to CLSI (2007) Statistical Analysis The data on total viable count and total coliform count of market and farm milk samples were analyzed in completely randomized design (CRD) and t test using SPSS software (Version 16, 2007). Correlation between total viable count and total coliform count were also evaluated by Pearson Correlation Coefficient method.

Source of Information: Turkish Journal of Agriculture - Food Science and Technology, 6(9): 1108-1113, 2018

Article Link (Online Journal): Available online, ISSN: 2148-127X www.agrifoodscience.com, Turkish Science and Technology

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present study indicated that the raw milk samples in the study area are highly contaminated in both farm and local markets. The problem of drug-resistant bacteria is a matter of concern globally especially in developing countries, where animals live in closer to humans, unaware people drink raw milk and milk products and chances of transmission of the drug-resistant pathogen from animal to human is high. The indiscriminate use of antibiotics in dairy animals and every step in milk collection, processing, marketing should be closely monitored. Molecular characterization of E. coli isolates from milk and diarrheal patients in the country is recommended to elucidate the role of raw milk as a source of human infection in Bangladesh and a routine survey of antibiotic resistance patterns is acknowledged in the country.

Knowledge Management: Journal