Amin T
Department of Anatomy and Histology, Bangladesh Agricultural University, Bangladesh
Afrin M
Department of Anatomy and Histology, Bangladesh Agricultural University, Bangladesh
Haque Z
Department of Anatomy and Histology, Bangladesh Agricultural University, Bangladesh
Islam MR
Department of Anatomy and Histology, Bangladesh Agricultural University, Bangladesh
Effluent, Haematology, Hepatomegally, Influent, Toxicity.
Effluent Treatment Plant (ETP) of a textile dye industry located at Valuka, Mymensingh
Risk Management in Agriculture
Waste, Efluent, Wastewater
2.1 Animals: Healthy, mature Swiss albino mice (Mus musculus) (age: 50-55 days), weighing 30-35g were acclimated 1 week prior to entry into the experimental protocol. Animals were housed in a well ventilated facility (temperature = 25±3 °C; humidity = 40-60% 12 h light:dark cycle) as per guidelines of the Institutional Ethical Committee and fed a standard diet provided by International Centre of Diarrheal Disease Research, Bangladesh (icddr, b) and tap water ad libitum. Thereafter, animals were divided into three groups including: control group, influent group (untreated textile dye wastewater) and effluent group (treated textile dye wastewater). Each group had five mice (three female and two male). These animals are treated with intraperitoneal injection of distilled water (control group), influent (influent group) and effluent (effluent group) for seven days.
2.2 Dye wastewater: The textile dye wastewater samples used during the present study were collected from an Effluent Treatment Plant (ETP) of a textile dye industry located at Valuka, Mymensingh and stored at 4°C during the study period. The waste water was analyzed in the laboratory of Department of Environment, Dhaka. The characteristics of the influent (untreated) and effluent (biologically treated) were as follows which were compared with normal water. In case of influent, level of Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) were higher than the limits set by Department of Environment, Bangladesh.
2.3 Assay of Liver Functions: The sera were separated from blood samples using cooling centrifugation and stored at 20°C until analysis. The above collected serum was used for the assay of marker enzymes of liver function; serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transaminase (SGPT).
2.4 Haematological Examination: Blood samples with anti coagulant EDTA (Ethelene-diamine tetra acetic acid) were analyzed for haematological parameters; total erythrocyte count (TEC), packed cell volume (PCV), hemoglobin (Hb) (g/dl) and erythrocyte sedimentation rate (ESR) in mm according to standard techniques.
2.5. Histopathological Examination: Specimens of liver were dissected from all animals immediately after killing, washed thoroughly with formal saline and then fixed in 10% neutral-buffered formal saline for 72 hours at least. All the specimens were washed in tap water for half an hour, dehydrated in ascending grades of alcohol (70-90-95% absolute), cleared in xylene and then embedded in paraffin wax. Serial sections of 6 um thick were cut and stained with Haematoxylin and eosin.
2.6. Statistics: The data are expressed as mean±SEM. Statistical tests (student's `t'-test; one and two way ANOVA) were applied to find significant difference between values of various parameters recorded for control and treated animals. The differences will considered to statistically significant When the p value obtained will less than 0.05 or 0.01,
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372. Volume 9, Issue 7 Ver. I (July 2016), PP 29-34 www.iosrjournals.org
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