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In Vitro Evaluation of the Performance of Different Plant Extracts Against the Growth of Mycoflora Associated with the Substrate of Oyster Mushroom

Moriom Akter Mousumi
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

Zehad Pervez*
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

Md. Shah Alam
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

Md. Shahidul Islam
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

Md. Rubel Mahmud
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

Abstract/ Executive Summary:

This study was conducted to know firstly about the prevalence of different mycoflora or weed molds associated with the substrate of oyster mushroom (Pleurotus ostreatus) and to evaluate the performance of different plant extracts against the isolated mycoflora. Ten weed molds including Aspergillus flavus, Aspergillus niger, Penicillium italicum, Penicillium notatum, Rhizopus stolonifer, Absidia sp., Fusarium sp., Chaetomium globosum, Chaetomium elatum, and Trichoderma harzianum were isolated and identified. From a number of 400 contaminated spawn packets Average 63.25% spawn packets were found contaminated with T. harzianum and remaining 36.75% spawn packets were found contaminated by other isolated mycoflora. An attempt was taken to determine antifungal potentials of different plant extracts viz. Onion (Allium cepa), Garlic (Allium sativum), Turmeric (Curcuma longa), Aloe vera (Aloe Barbadensis), Neem (Azadirechta indica), Lantana (Lantana camara), Tulsi (Ocimum tenuiflorum), Datura (Datura stramonium) at 5% concentration against isolated weed molds. Neem leaf extract at the concentration of 5% inhibits the percent radial growth of all mycoflora (ranging from 55.26% to 67.87%) radial growth inhibition which perform the best. In compatibility test of different plant extracts at 5% concentration with P. ostreatus, it is revealed that extracts of neem, lantana and datura were found to be compatible with P. ostreatus. Turmeric and onion were highly incompatible and significantly inferior.

Keywords: Mycoflora, Oyster mushroom, Plant extracts.

Location: Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Extract (plant, seed), Mushroom

Objectives:

Extracts of many plants have been reported to exhibit antibacterial, antifungal and insecticidal properties under laboratory trial. Plant metabolites and plant-based pesticides appear to be one of the better alternatives as they are known to have minimal environmental impact and Danger to consumers in contrast to synthetic pesticides. In recent years, the antimicrobial properties of plant extracts have been reported with increasing frequency from different parts of the world. Plant products as antimicrobial agents. The present study was carried out to identify the major mycoflora associated with the substrate of oyster mushrooms and evaluate the inhibition efficacy of different plant extracts against associated mycoflora

Methodology:

Widely cultivated species Pleurotus ostreatus of oyster mushroom was selected to conduct the present experiment. Commercial spawn packet of oyster mushroom (Pleurotus ostreatus) was collected from National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh. Pure culture was grown by taking tissues from the fruiting bodies and subsequently mother culture was prepared by using the pure culture. A number of 400 contaminated Pleurotus ostreatus (Oyster mushroom) growing substrate packets were collected from different mushroom farms at Savar Upazilla. Pure cultures of different associated mycoflora were isolated by inoculating a small portion of contaminated substrates to PDA media. Eight commonly available plants viz. Onion, Garlic, Turmeric, Aloe vera, Neem, Lantana, Tulsi and Datura were collected as inhibition agents and were prepared extracts for evaluating inhibition efficiency against mycoflora associated with the substrate of oyster mushroom. 5% concentration of different plant extracts viz. Onion, Garlic, Turmeric, Aloe vera, Neem, Lantana, Tulsi and Datura were tested in vitro to evaluate their effect on the radial growth of isolated fungi.Efficiency of plant extracts were evaluated on the basis of percent inhibition of the radial colony growth of the isolated fungi, and computed as a proportional measurement of colony diameter on control plate expressing in percentage, following the given formula.

% inhibition = X-Y / X x100

Where, X= radial growth (mm) of control plates, Y = radial growth (mm) of fungicide treated plates

5% concentration of Onion, Garlic, Turmeric, Aloe vera, Neem, Lantana, Tulsi and Datura were used to determine their effect on growth of P. ostreatus following poison food technique. The recorded data for each character from the experiment were analyzed statistically using MSTAT C program. The mean from all the treatments were calculated and analysis of variance of characters under study was performed by F variance test. The mean differences were evaluated by Duncan’s Multiple Range Test (DMRT).

Source of Information: IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-ISSN: 2319-2380, p-ISSN: 2319-2372. Volume 10, Issue 1 Ver. I (January. 2017), PP 33-36 www.iosrjournals.org

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Currently, the use of inhibitory botanicals is one of the most possible methods for controlling some plant pathogens. A total number of 10 weed molds namely, Aspergillus flavus, Aspergillus niger, Penicillium italicum, Penicillium notatum, Rhizopus stolonifer, Absidia sp., Fusarium sp., Chaetomium globosum, Chaetomium elatum, and Trichoderma harzianum were isolated, identified and found to infect surveyed Oyster mushroom substrate. .This study found most promising botanic Neem (Azadirechta indica), at 5% neem was found highly effective in inhibiting significantly the growth of all the associated weed molds. For Neem extract contamination spawn packets is only 5%.On the other hand,5% neem extract is also compatible for colony growth of Pleurotus ostreatus.

Knowledge Management: Journal