Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Callus Induction and Plant Regeneration from Immature Zygotic Embryos of Black Pepper (piper Nigrum L.)

M.A. Rahman
Agricultural University College, BAU campus Mymensingh-2202,

L A. Khondaker
Department of Botany, University of Rajshahi

0.I. Joarder
Department of Genetics and Breeding, University of Rajshahi-6205

Abstract/ Executive Summary:

Immature zygotic embryos of Piper nigrum L. were cultured in different concentrations and a combination of auxins and cytokinins. Callus formation was highest (92.2%) when the explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/I IAA and 0.5 mg/I kinetin. CaIli were transferred to same basal medium containing 1.5 mg/I BAP and 0.5 mg/I NAA, where they developed a maximum number of adventitious buds within three weeks of culture. Regenerated shoots were elongated on MS medium containing 1.5 mg/I BAP and 0.5 mg/I NAA and subsequently rooted in half-strength MS medium with 1.5 mg/I IBA.

Keywords: Black pepper, Callus induction, Shoot regeneration, Root induction

Location: Biotechnology Laboratory of the Institute of Biological Sciences, University of Rajshahi, Rajshahi

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Regeneration, Callus, Black pepper

Objectives:

Very little information is available on in vitro culture in black pepper (Rahman et aL 2000; Rahman 2006). The tissue culture technique could offer a valuable alternative and reliable procedure for the large-scale propagation of black pepper. The present study describes a technique for the production of multiple shoots through callus culture from immature zygotic embryos of black pepper.

Methodology:

The experiment was carried out in the Biotechnology Laboratory of the Institute of Biological Sciences, University of Rajshahi, Rajshahi. After surface sterilization seeds were air-dried and excised embryos were placed in 100x15 mm petridishes containing an agar-gelled medium for callus induction. The dishes were sealed with parafilm and stored in dark at 26±1°C. Cultures were scored for number of embryos induced callus and days to callus initiation. The growth media consisted of MS basal formula (Murashige and Skoog, 1962) with 0.5 - 4.0 mg/I IAA. With every concentration of IAA, 0.5 mg/I kinetin was also used. The proliferated calli were subcultured in MS medium containing different concentrations ( 0.5 - 4.0 mg/I ) of BAP with 0.5 mg/I NAA .for adventitious shoot bud induction. The elongated shoots (>3.0 cm long) were placed for 'rooting in half-strength MS medium with IBA and IAA in different concentrations.

The data on both callus induction and shoot regeneration frequency (%) were recorded after four weeks of inoculation and the results are presented as mean ± standard error with analysis of variance. The experiments were carried out in a Randomized Complete Block (RCB) design.

Source of Information: J. Bangladesh Agril. Univ. 5(1): 87-90, 2007 ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The regenerated shoots were excised from stock cultures and subcultures individually into medium containing 1.5 mg/I BAP and 0.5 mg/I NAA for shoot elongation. The elongated shoots (> 3 cm long) were rooted in half-strength MS medium containing various concentrations of IBA and IAA. IBA was found to be more effective in respect of root induction, and 82.5% shoots produced roots within in media having 1.5 mg/I IBA and mean number of roots per shoot was 6.8. Rooted plantlets were transferred to pots and more than 88% plants survived. The present investigation provides a method that ensures a multiple shoot induction from immature zygotic embryos explants of black pepper via intermediate callus phase.

Knowledge Management: Journal