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Seed Germination and Seedling Growth of Brinjal, Tomato and Chilli Treated with Ga3 and Gaba

M. Obaidul Islam
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

M. Serazul Islam
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

A.K.M. Azad-Ud-Doula Prodhan
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

University, Mymensingh, during the period from May 2003 to March 2004 to investigate the effect of GA3 and GABA on seed germination and seedling growth of brinjal, tomato and chilli. The concentrations of GABA were 0.16 mll.:1, 0.33 m1L-1, 0.66 mIL:1 and of GA3 were 25 mgL-1, 50 mgC, 100 mg1:1 and of water respectively along with control where only water was used to treat seeds by soaking for 12 hours prior to sowing on saturated tissue paper in petri-dishes. The experiment was laid out following RCBD design where each treatment was replicated five times. The germination percentage of all the seeds treated with PGRs of the above-mentioned concentrations was significantly higher over control. Seed germination was enhanced significantly in brinjal and tomato at 100 mgL-1 of GA3. Seedling growth (shoot length and root length) increased at 25, 100 mgL-1 of GA3 and 0.33 mgL-1 of GABA.

Keywords: GABA, Growth regulator, Seed germination, Seedling growth, Germination percentage

Location: Department of Crop Botany, Bangladesh Agricultural University, Mymensingh

Start Date: 00-05-2003

End Date: 00-03-2004

Program Area: Seed Technology

Commodity: Seed germination, Gibberellic acid (GA3), Vegetables

Objectives:

The application of suitable plant growth regulators is more convenient as well as economic as it needs a very small quantity. Application of PGR does not require any special technique or expensive procedure. In Bangladesh, the effects of GABA on germination and growth of brinjal, tomato and chili (day-neutral plants) are not reported. Hence the present research was planned to investigate the effect of GABA and GA3 on seed germination of those crops and to observe the effective comparison between these two PGRs

Methodology:

Plant materials design and methodology Disease-free, clean and fresh seeds of brinjal, tomato and chilli were collected form BADC office of Mymensingh and used in the present study. Seeds were treated for 12 hours by soaking in aqueous solution of GABA at 0.16, 0.33 and 0.66 mIL-1 and Gibberellic acid (GA3) at 25, 50 and 100 mgL-1 along with control where only water was used and then uniformly sown on 4 ply of tissue paper in plastic petri dishes of 20 cm in diameter. For each species, two hundred seeds were sown on germinating papers keeping moist always by supplying tap water manually. The experiment was laid out in single factorial Randomized Complete Block Design (RCBD) where each treatment was replicated five times. The average monthly temperatures of day and night were 30°C and 27°C respectively,

Data collection on seed germination, seedling growth and analysis germination was considered the emergence of radicle. Data on the emergence were taken every day with 24 hours intervals starting from sowing and continuing up to the completion of germination. The percentage of germination was calculated. For sampling, ten seedlings were selected randomly aside from each petri dishes from 3 days after sowing (DAS) and continued up to 15 days old with an interval of 3 days. The shoot length (cm), root length (cm), and fresh weight of seedlings (gm) were recorded immediately after uprooting the seedlings.

Analysis of variance was calculated using the computer software programme MSTAT-C (Russell, 1986) and the statistical analysis was done following single factorial Randomized Complete Block Design. The co-efficient of variation in treatment was calculated to measure the precision of the experiment and the treatment means were compared by Duncan's Multiple Range test (Gomez and Gomez, 1984).

Source of Information: J. Bangladesh Agri!. Univ. 4(1): 43-49, 2006 ISSN 1810-3030

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Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The experiment was laid out following RCBD design where each treatment was replicated five times. The germination percentage of all the seeds treated with PGRs of the above-mentioned concentrations was significantly higher over control. Seed germination was enhanced significantly in brinjal and tomato at 100 mgL-1 of GA3. Seedling growth (shoot length and root length) increased at 25, 100 mgL-1 of GA3 and 0.33 mgL-1 of GABA.

Knowledge Management: Journal