M.S.R. Suman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M. B. Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
S.M.L. Kabir
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
K.A. Choudhury
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M.N.A. Siddiky
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Efficacy, Formalin and heat killed, Fowl cholera vaccines
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Comparative study
Pasteurella multocida vaccine strain: Pasteurella nzultocida organisms were obtained from laboratory stock culture of the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Birds: Twenty-five healthy Fayoumi birds of 2 months age were selected for this study and these birds were collected from the Bangladesh Agricultural University Poultry Farm, Mymensingh, Bangladesh.
Cultural examination of P. multocida: Cultural examination was conducted according to the standard method described by Cowan (1985). The collected organisms were inoculated in blood agar media, nutrient agar media, and MacConkey agar media to observe the growth characteristics of the organisms. Biochemical tests were also performed for confirmation of the organism according to the methods described by Cheesbrough (1985) and Cowan (1985).
Preparation of FK FC and HK FC vaccines: Formaldehyde was added at the rate of 0.8% of bacterial culture (3.8x108 CFU/ ml) for FK FC vaccine preparation and bacterial suspension was heated by keeping at 600 C for half an hour in a water bath for HK FC vaccine preparation. Later, 1 ml of each treated bacterial culture was inoculated on Blood agar media separately and incubated at 37°C for overnight. The plate with no bacterial growth indicated complete inactivation and was negative for bacterial contamination.
Experimental immunization: The experimental chickens were divided into five major groups. Birds of groups A and B were vaccinated with experimentally prepared FK FC vaccine and groups C and D were vaccinated with experimentally prepared HK FC vaccine and the remaining group E was served as control. The birds of group A and group C were vaccinated through IM route and group B and group D were vaccinated through SC route. Each group of chickens was revaccinated with the same vaccine using the same route after two weeks of the primary vaccination as a booster dose.
Collection of serum from the immunized birds: Five ml of blood was collected from the wing vein of all vaccinated chickens of each group without anticoagulant and was poured gently in the sterile glass test tubes. Blood samples were collected from the chickens at prevaccination and two weeks after each vaccination and two weeks after challenge infection. Sera were separated from the blood and stored at —20°C in the screw-capped vial until tested.
Determination of the humoral immune response: Humoral immune response was determined by the application of slide agglutination test (SAT), growth inhibition test (GIT) and passive hemagglutination assay (PHA). Antibody titers of the chickens that were immunized with FK FC and HK FC vaccines was determined by modified passive hemagglutination assay according to the method described by Tripathy et al. (1970), Siddique et al. (1997).
Protection test: Five chickens from each four vaccinated and one non-vaccinated group were challenged intramuscularly with 1 ml of live P. nzultocida bacteria (3.8 x 108 CFU/ml) for the protection test.
J. Bangladesh Agril. Univ. 2(1): 81-85,2004 ISSN 1810-3030
Journal